LOCUS       sequence-328032-       12288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from 
            the UbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, 
            Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH,
            Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
  TITLE     Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors 
            promotes axon survival.
  JOURNAL   J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 
            10.1083/jcb.202005051.
  PUBMED    33284322
REFERENCE   2  (bases 1 to 12288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2021-01-4"; volume: "220(1). pii: 211577. doi"; 
            pages: " 10.1083/jcb.202005051"
COMMENT     Sequence Label: UbC-Halo-SFPQY527A
FEATURES             Location/Qualifiers
     source          1..12288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1481..1525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2005..3216
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3053..3072
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3262..3271
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3268..4155
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     misc_feature    6363..6951
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6416..6436)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6834..6845)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6954..6970)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6955..6971)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7476..7656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7682..7699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7688..7912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7958..8386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8045..8064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8255..8276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8395..8415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8400..8729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8580..8715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8642..8661
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8777..8824
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8843..9217
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9347..9468
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9384..9403)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9438..9457
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9517..9533)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9517..9533)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9530..9552)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9541..9557
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9565..9571,9572..9589,9590..9595))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9610..9631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9748..9765)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9919..10507)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9999..10018)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10678..11469,11470..11538))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11301..11320
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11539..11643)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11718..11737)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11909..12288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttaacccgtg tcggctccag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     2041 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     2101 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     2161 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     2221 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     2281 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     2341 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     2401 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     2461 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     2521 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     2581 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     2641 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     2701 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     2761 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     2821 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     2881 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     2941 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     3001 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     3061 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     3121 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     3181 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc aggtcgactc
     3241 tagaggatcc ccgggtaccg ggccaccatg gaaatcggta ctggctttcc attcgacccc
     3301 cattatgtgg aagtcctggg cgagcgcatg cactacgtcg atgttggtcc gcgcgatggc
     3361 acccctgtgc tgttcctgca cggtaacccg acctcctcct acgtgtggcg caacatcatc
     3421 ccgcatgttg caccgaccca tcgctgcatt gctccagacc tgatcggtat gggcaaatcc
     3481 gacaaaccag acctgggtta tttcttcgac gaccacgtcc gcttcatgga tgccttcatc
     3541 gaagccctgg gtctggaaga ggtcgtcctg gtcattcacg actggggctc cgctctgggt
     3601 ttccactggg ccaagcgcaa tccagagcgc gtcaaaggta ttgcatttat ggagttcatc
     3661 cgccctatcc cgacctggga cgaatggcca gaatttgccc gcgagacctt ccaggccttc
     3721 cgcaccaccg acgtcggccg caagctgatc atcgatcaga acgtttttat cgagggtacg
     3781 ctgccgatgg gtgtcgtccg cccgctgact gaagtcgaga tggaccatta ccgcgagccg
     3841 ttcctgaatc ctgttgaccg cgagccactg tggcgcttcc caaacgagct gccaatcgcc
     3901 ggtgagccag cgaacatcgt cgcgctggtc gaagaataca tggactggct gcaccagtcc
     3961 cctgtcccga agctgctgtt ctggggcacc ccaggcgttc tgatcccacc ggccgaagcc
     4021 gctcgcctgg ccaaaagcct gcctaactgc aaggctgtgg acatcggccc gggtctgaat
     4081 ctgctgcaag aagacaaccc ggacctgatc ggcagcgaga tcgcgcgctg gctgtctact
     4141 ctggagattt ccggtggcgc cccgtacaag atgtctcggg atcggttccg gagtcgtggc
     4201 ggtggcggtg gtggcttcca caggcgtgga ggaggcggcg gccgcggcgg cctccacgac
     4261 ttccgttctc cgccgcccgg catgggcctc aatcagaatc gcggccccat gggtcctggc
     4321 ccgggccaga gcggccctaa gcctccgatc ccgccaccgc ctccacacca acagcagcaa
     4381 cagccaccac cgcagcagcc accgccgcag cagccgccac cgcatcagcc gccgccgcat
     4441 ccacagccgc atcagcagca gcagccgccg ccaccgccgc aggactcttc caagcccgtc
     4501 gttgctcagg gacccggccc cgctcccgga gtaggcagcg caccaccagc ctccagctcg
     4561 gccccgcccg ccactccacc aacctcgggg gccccgccag ggtccgggcc aggcccgact
     4621 ccgaccccgc cgcctgcagt cacctcggcc cctcccgggg cgccgccacc caccccgcca
     4681 agcagcgggg tccctaccac acctcctcag gccggaggcc cgccgcctcc gcccgcggca
     4741 gtcccgggcc cgggtccagg gcctaagcag ggcccaggtc cgggtggtcc caaaggcggc
     4801 aaaatgcctg gcgggccgaa gccaggtggc ggcccgggcc taagtacgcc tggcggccac
     4861 cccaagccgc cgcatcgagg cggcggggag ccccgcgggg gccgccagca ccacccgccc
     4921 taccaccagc agcatcacca ggggcccccg cccggcgggc ccggcggccg cagcgaggag
     4981 aagatctcgg actcggaggg gtttaaagcc aatttgtctc tcttgaggag gcctggagag
     5041 aaaacttaca cacagcgatg tcggttgttt gttgggaatc tacctgctga tatcacggag
     5101 gatgagttca aaagactatt tgctaaatat ggagaaccag gagaagtttt tatcaacaaa
     5161 ggcaaaggat tcggatttat taagcttgaa tctagagctt tggctgaaat tgccaaagcc
     5221 gaactggatg atacacccat gagaggtaga cagcttcgag ttcgctttgc cacacatgct
     5281 gctgcccttt ctgttcgtaa tctttcacct tatgtttcca atgaactgtt ggaagaagcc
     5341 tttagccaat ttggtcctat tgaaagggct gttgtaatag tggatgatcg tggaagatct
     5401 acagggaaag gcattgttga atttgcttct aagccagcag caagaaaggc atttgaacga
     5461 tgcagtgaag gtgttttctt actgacgaca actcctcgtc cagtcattgt ggaaccactt
     5521 gaacaactag atgatgaaga tggtcttcct gaaaaacttg cccagaagaa tccaatgtat
     5581 caaaaggaga gagaaacccc tcctcgtttt gcccagcatg gcacgtttga gtacgaatat
     5641 tctcagcgat ggaagtcttt ggatgaaatg gaaaaacagc aaagggaaca agttgaaaaa
     5701 aacatgaaag atgcaaaaga caaattggaa agtgaaatgg aagatgccgc tcatgaacat
     5761 caggcaaatc ttttgcgcca agatctgatg agacgacagg aagaattaag acgcatggaa
     5821 gaacttcaca atcaagaaat gcagaaacgt aaagaaatgc aattgaggca agaggaggaa
     5881 cgacgtagaa gagaggaaga gatgatgatt cgtcaacgtg agatggaaga acaaatgagg
     5941 cgccaaagag aggaaagtta cagccgaatg ggctacatgg atccacggga aagagacatg
     6001 cgaatgggtg gcggaggagc aatgaacatg ggagatccct atggttcagg aggccagaaa
     6061 tttccacctc taggaggtgg tggtggcata ggttatgaag ctaatcctgg cgttccacca
     6121 gcaaccatga gtggttccat gatgggaagt gacatgcgta ctgagcgctt tgggcaggga
     6181 ggtgcggggc ctgtgggtgg acagggtcct agaggaatgg ggcctggaac tccagcagga
     6241 tatggtagag ggagagaaga gtacgaaggc ccaaacaaaa aaccccgatt ttagggcgcg
     6301 cccctgcagg caccacactg gactagtgct agcgttaaca attcgatatc aagcttatcg
     6361 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6421 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6481 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6541 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6601 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6661 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6721 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     6781 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6841 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6901 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     6961 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7021 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7081 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7141 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7201 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7261 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     7321 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     7381 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     7441 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7501 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7561 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7621 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7681 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7741 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7801 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7861 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7921 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7981 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8041 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8101 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8161 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8221 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8281 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8341 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     8401 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8461 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8521 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8581 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8641 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8701 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8761 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8821 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8881 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8941 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9001 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9061 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9121 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9181 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     9241 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9301 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9481 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9541 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9601 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9721 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9781 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9841 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9901 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9961 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10021 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10081 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10141 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10201 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10261 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10321 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10381 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10441 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10501 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10561 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10621 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10681 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10741 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10801 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10861 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10921 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10981 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11041 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11101 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11161 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11221 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11281 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11341 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11401 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11461 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11521 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11641 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    11701 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    11761 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    11821 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    11881 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
    11941 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
    12001 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
    12061 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
    12121 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
    12181 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
    12241 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
