LOCUS       sequence-328037-        8786 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #8 TCRbeta cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8786)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRb_candidate8
FEATURES             Location/Qualifiers
     source          1..8786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      608..617
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1597..2185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1650..1670)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2068..2079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2257..2490
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2568..2689
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2605..2624)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2659..2678
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2729..2864
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2884..2903)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2885..2903)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2913..2930)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2913..2929)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2922..2944)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3071..3526
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3158..3177)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3368..3389
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3552..3656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3657..3725,3726..4517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3875..3894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4688..5276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5177..5196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5430..5447
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5564..5585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5600..5605,5606..5623,5624..5630)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5638..5654
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5643..5665
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5662..5678
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5662..5678
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5697..5717
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5699..5717
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5745..5971
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5972..6152
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6199..6324
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6817..7050
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7235..7279
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7454..7954
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7873..7891
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7976..8575
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7976..7995)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8472..8492
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8617..8734
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtgccgcc accatggact cctggacctt ctgctgtgtg tccctttgca tcctggtagc
      661 gaagcataca gatgctggag ttatccagtc accccgccat gaggtgacag agatgggaca
      721 agaagtgact ctgagatgta aaccaatttc aggccacaac tcccttttct ggtacagaca
      781 gaccatgatg cggggactgg agttgctcat ttactttaac aacaacgttc cgatagatga
      841 ttcagggatg cccgaggatc gattctcagc taagatgcct aatgcatcat tctccactct
      901 gaagatccag ccctcagaac ccagggactc agctgtgtac ttctgtgcca gcagtttagg
      961 cggcgccggc gatacgcagt attttggccc aggcacccgg ctgacagtgc tcgaggacct
     1021 gaaaaacgtg ttcccacccg aggtcgctgt gtttgagcca tcagaagcag agatctccca
     1081 cacccaaaag gccacactgg tgtgcctggc cacaggcttc ttccctgacc acgtggagct
     1141 gagctggtgg gtgaatggga aggaggtgca cagtggggtc tgcacggacc cgcagcccct
     1201 caaggagcag cccgccctca atgactccag atactgcctg agcagccgcc tgagggtctc
     1261 ggccaccttc tggcagaacc cccgcaacca ctttcgatgc caggtacaat tttatggtct
     1321 ctcggagaat gacgagtgga cccaggatag ggccaaaccc gtcacccaga tcgtcagcgc
     1381 cgaggcctgg ggtagagcag actgtggctt tacctcggtg tcctaccagc aaggggtcct
     1441 gtctgccaca atcctctatg agatcctgct agggaaggcc accctgtatg ctgtgctggt
     1501 cagcgccctt gtgttgatgg ccatggtcaa gagaaaggat ttctgaaaag tggtttagta
     1561 atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     1621 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     1681 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     1741 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     1801 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     1861 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     1921 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     1981 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2041 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2101 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2161 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     2221 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2281 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2341 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2401 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2461 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     2521 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     2581 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2641 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     2701 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     2761 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     2821 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     2881 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     2941 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3001 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3061 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3121 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3181 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3241 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3301 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3361 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3421 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3481 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4201 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4801 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5281 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5401 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5461 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     5521 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     5581 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     5641 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     5701 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     5761 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     5821 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     5881 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     5941 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6001 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6061 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6121 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6181 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6241 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6301 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6361 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6421 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6481 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     6541 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     6601 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     6661 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     6721 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     6781 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     6841 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     6901 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     6961 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7021 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7081 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7141 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7201 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     7261 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7321 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7381 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7441 cccgagggga cccttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg
     7501 ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac
     7561 gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct
     7621 tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca
     7681 aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc
     7741 agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag
     7801 cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt
     7861 cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc
     7921 cctcgttgac cgaatcaccg acctctctcc ccagggggta ccaccggagc ttaccatgac
     7981 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8041 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8101 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8161 caaggtgtgg gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt
     8221 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8281 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8341 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8401 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8461 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8521 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagaatt
     8581 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     8641 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     8701 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     8761 tccactttgg cgccggctcg aggggg
//
