LOCUS       sequence-328367-        8095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian cell expression vector for His-tagged DPP9S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8095)
  AUTHORS   Hollingsworth LR, Sharif H, Griswold AR, Fontana P, Mintseris J, 
            Dagbay KB, Paulo JA, Gygi SP, Bachovchin DA, Wu H
  TITLE     DPP9 sequesters the C terminus of NLRP1 to repress inflammasome 
            activation.
  JOURNAL   Nature. 2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4.
  PUBMED    33731932
REFERENCE   2  (bases 1 to 8095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2021 Mar 17. pii: 10.1038/s41586-021-03350-4. doi: 
            10.1038/s41586-021-03350-4."
COMMENT     Sequence Label: pcDNA3.1 LIC 6A HIS-TEV-DPP9S
FEATURES             Location/Qualifiers
     source          1..8095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             947..964
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             986..1006
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     promoter        complement(3648..3666)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3649..3666)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3686..3703)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3692..3916
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3962..4390
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4049..4068)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4259..4280
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4399..4419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4404..4733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4584..4719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4646..4665
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4800..5594
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4854..4873)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5464..5483
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5768..5889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5805..5824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5859..5878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5938..5954)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5938..5954)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5951..5973)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5962..5978
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5986..5992,5993..6010,6011..6016))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6031..6052
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6169..6186)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6340..6928)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6420..6439)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7099..7890,7891..7959))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7722..7741
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7960..8064)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccctacttcc aatccaatgc caccatgtcg tactaccatc accatcacca
      961 tcacgattac gatatcccaa cgaccgaaaa cctgtatttt cagggcgcca tgggatccat
     1021 ggccaccacc gggaccccaa cggccgaccg aggcgacgca gccgccacag atgacccggc
     1081 cgcccgcttc caggtgcaga agcactcgtg ggacgggctc cggagcatca tccacggcag
     1141 ccgcaagtac tcgggcctca ttgtcaacaa ggcgccccac gacttccagt ttgtgcagaa
     1201 gacggatgag tctgggcccc actcccaccg cctctactac ctgggaatgc catatggcag
     1261 ccgagagaac tccctcctct actctgagat tcccaagaag gtccggaaag aggctctgct
     1321 gctcctgtcc tggaagcaga tgctggatca tttccaggcc acgccccacc atggggtcta
     1381 ctctcgggag gaggagctgc tgagggagcg gaaacgcctg ggggtcttcg gcatcacctc
     1441 ctacgacttc cacagcgaga gtggcctctt cctcttccag gccagcaaca gcctcttcca
     1501 ctgccgcgac ggcggcaaga acggcttcat ggtgtcccct atgaaaccgc tggaaatcaa
     1561 gacccagtgc tcagggcccc ggatggaccc caaaatctgc cctgccgacc ctgccttctt
     1621 ctccttcatc aataacagcg acctgtgggt ggccaacatc gagacaggcg aggagcggcg
     1681 gctgaccttc tgccaccaag gtttatccaa tgtcctggat gaccccaagt ctgcgggtgt
     1741 ggccaccttc gtcatacagg aagagttcga ccgcttcact gggtactggt ggtgccccac
     1801 agcctcctgg gaaggttcag agggcctcaa gacgctgcga atcctgtatg aggaagtcga
     1861 tgagtccgag gtggaggtca ttcacgtccc ctctcctgcg ctagaagaaa ggaagacgga
     1921 ctcgtatcgg taccccagga caggcagcaa gaatcccaag attgccttga aactggctga
     1981 gttccagact gacagccagg gcaagatcgt ctcgacccag gagaaggagc tggtgcagcc
     2041 cttcagctcg ctgttcccga aggtggagta catcgccagg gccgggtgga cccgggatgg
     2101 caaatacgcc tgggccatgt tcctggaccg gccccagcag tggctccagc tcgtcctcct
     2161 ccccccggcc ctgttcatcc cgagcacaga gaatgaggag cagcggctag cctctgccag
     2221 agctgtcccc aggaatgtcc agccgtatgt ggtgtacgag gaggtcacca acgtctggat
     2281 caatgttcat gacatcttct atcccttccc ccaatcagag ggagaggacg agctctgctt
     2341 tctccgcgcc aatgaatgca agaccggctt ctgccatttg tacaaagtca ccgccgtttt
     2401 aaaatcccag ggctacgatt ggagtgagcc cttcagcccc ggggaagatg aatttaagtg
     2461 ccccattaag gaagagattg ctctgaccag cggtgaatgg gaggttttgg cgaggcacgg
     2521 ctccaagatc tgggtcaatg aggagaccaa gctggtgtac ttccagggca ccaaggacac
     2581 gccgctggag caccacctct acgtggtcag ctatgaggcg gccggcgaga tcgtacgcct
     2641 caccacgccc ggcttctccc atagctgctc catgagccag aacttcgaca tgttcgtcag
     2701 ccactacagc agcgtgagca cgccgccctg cgtgcacgtc tacaagctga gcggccccga
     2761 cgacgacccc ctgcacaagc agccccgctt ctgggctagc atgatggagg cagccagctg
     2821 ccccccggat tatgttcctc cagagatctt ccatttccac acgcgctcgg atgtgcggct
     2881 ctacggcatg atctacaagc cccacgcctt gcagccaggg aagaagcacc ccaccgtcct
     2941 ctttgtatat ggaggccccc aggtgcagct ggtgaataac tccttcaaag gcatcaagta
     3001 cttgcggctc aacacactgg cctccctggg ctacgccgtg gttgtgattg acggcagggg
     3061 ctcctgtcag cgagggcttc ggttcgaagg ggccctgaaa aaccaaatgg gccaggtgga
     3121 gatcgaggac caggtggagg gcctgcagtt cgtggccgag aagtatggct tcatcgacct
     3181 gagccgagtt gccatccatg gctggtccta cgggggcttc ctctcgctca tggggctaat
     3241 ccacaagccc caggtgttca aggtggccat cgcgggtgcc ccggtcaccg tctggatggc
     3301 ctacgacaca gggtacactg agcgctacat ggacgtccct gagaacaacc agcacggcta
     3361 tgaggcgggt tccgtggccc tgcacgtgga gaagctgccc aatgagccca accgcttgct
     3421 tatcctccac ggcttcctgg acgaaaacgt gcactttttc cacacaaact tcctcgtctc
     3481 ccaactgatc cgagcaggga aaccttacca gctccagatc taccccaacg agagacacag
     3541 tattcgctgc cccgagtcgg gcgagcacta tgaagtcacg ttgctgcact ttctacagga
     3601 atacctctag taataacatt ggaagtggat aatctagagg gccctattct atagtgtcac
     3661 ctaaatgcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3721 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3781 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3841 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3901 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     3961 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4021 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4081 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4141 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4201 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4261 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4321 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4381 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4441 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4501 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4561 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4621 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     4681 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     4741 cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca
     4801 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     4861 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     4921 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     4981 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     5041 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     5101 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     5161 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     5221 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     5281 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     5341 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     5401 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     5461 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     5521 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     5581 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     5641 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     5701 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     5761 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5881 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     5941 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6001 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6061 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6121 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6181 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6241 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     6301 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6361 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6421 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6481 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     6541 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     6601 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     6661 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     6721 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     6781 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     6841 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     6901 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6961 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7021 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7081 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7141 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7201 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7261 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7321 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7381 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7441 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7501 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7561 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7621 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7681 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7741 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7801 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7861 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7921 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcattatta
     7981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
