LOCUS       sequence-32841-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
            necessary for its activation by amino acids.
  JOURNAL   Cell. 2010 Apr 16. 141(2):290-303.
  PUBMED    20381137
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 16. 141(2):290-303."
COMMENT     Sequence Label: pLKO.1 p18 shRNA
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    135..252
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        301..811
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     730..748
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(833..852)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ttcttggctt tatatatctt gtggaaggac gaggtaccgg agacagccag caacatcatt
       61 gctcgagcaa tgatgttgct ggctgtcttt tttgaattct agatcttgag acaaatggca
      121 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      181 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      241 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag
      301 ggggcccggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
      361 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
      421 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
      481 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
      541 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
      601 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
      661 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
      721 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
      781 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga
      841 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccaggg
//
