LOCUS       sequence-32842-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Sancak Y, Bar-Peled L, Zoncu R, Markhard AL, Nada S, Sabatini DM
  TITLE     Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is
            necessary for its activation by amino acids.
  JOURNAL   Cell. 2010 Apr 16. 141(2):290-303.
  PUBMED    20381137
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 16. 141(2):290-303."
COMMENT     Sequence Label: pLKO.1 MP1 shRNA
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     124..143
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    298..415
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
       61 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      121 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      181 ttgtggaaag gacgaggtac cgggagatgg agtacctgtt attactcgag taataacagg
      241 tactccatct ctttttgaat tctagatctt gagacaaatg gcagtattca tccacaattt
      301 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      361 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      421 ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc gggttgcgcc
      481 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
      541 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
      601 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
      661 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
      721 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
      781 ctgtggccaa tagcggctgc
//
