LOCUS       sequence-328542-        6926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Blasticidin resistance vector with attB and eye marker. Contains an 
            Alpha GB cloning entry point for addition of custom assembly uses 
            blue-white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pBlastR-GMRWhite
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1315..1336)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    1763..1832
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     1889..1908
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2102..2124
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2116..2133
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2117..2133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2140..2159
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2140..2158
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2161..2217
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(2224..2242)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2225..2242)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(2296..2343)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(2367..2822)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2504..2525)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2716..2735
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(2877..3668)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4136..4724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4625..4644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5039..5173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5076..5095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5130..5149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gatatggcgc tcattgcaga acagtccgcg ggtgcggttc accagctgac gccagccact
       61 gcctggctgg ttcacggcgc cgaagatatc catgttatgc caggcgtacg tcagattctc
      121 ggccagctgt ccggatccgc ttccgctatc ctctgggggg ctggggggca gcagggttcc
      181 gtaattcttg gcctggccaa atccctgatt gatgcacgac tggctggcgg ctccgctatc
      241 gccgttgttc aggtgctcgg cgcttgggtg cttggagcct ccgcggatca gcagctcctg
      301 gtcctcctgt cccatttggt ttggtatgcg tcttgtgatt caaagttggc ttattcaaag
      361 gatattaacg aaggcagcgg cacgtctgct cggctcgaac attcattcac aactgatgct
      421 ctcagccacc ccgcgccctt ttataccgct gcgctcgatc cccgggcgag ctcgggaggg
      481 gcatgacgta cctacctcga ctgctactcg acttaaaggc atttcaaggg tttccactgg
      541 gctcgactta aaggcatttc aagggtttcc actgggctcg acttaaaggc atttcaaggg
      601 tttccactgg gctcgactta aaggcatttc aagggtttcc actgggctcg acttaaaggc
      661 atttcaaggg tttccactgg gctcgacagt caggtgctag tcgtacgctc ctgacagcgg
      721 aacaaacgac ccaacacccg tgcgttttat tctgtctttt tattgccaag cttagttacg
      781 ggtatacttc aggggaatca gttcctcaat ggtggtcttg accagcttgc cgttcatctc
      841 aatcagcacg aagcaatcgg gggcgtaatc gctaatcagc tcacggcaca ttccacacgg
      901 ggacacgaca cggatgctac ggtccacctc atcggagtat gggtgacgca cggccacaat
      961 ggtatcaaaa tccttctggc cgttgctcac ggcactgcca atggcaatgg cctcggcaca
     1021 cacggtcacg cgtccgatgt aggcctcaat gtgcacggca ctgataatct cgcccgtctt
     1081 ggtgcgaatg gcagctccca cgtggtgctt gttatcctcg tagagcatcg taatcttctc
     1141 ggtagccacc tccaccagtt ccaggtcctg ctggctgatg ttgaaggttt tcattttgtt
     1201 ttcctcctta tgttaagctt actcagttat tatatcataa atatctgtgt caagaataaa
     1261 ctcccacatg gattcgagta ttgagtgttc tcttcttgta ttcaataatt acttcttggc
     1321 agatttcagt agttgcagtt gatttacttg gttgctggtt acttttaatt gattcacttt
     1381 aacttgcact ttactgcaga ttgtttagct tgttcagctg cgcttgttta tttgcttagc
     1441 tttcgcttag cgacgtgttc actttgcttg tttgaattga attgtcgctc cgtagacgaa
     1501 gcgcctctat ttatactccg gcgctctttt cgcgaacatt cgaggcgcgc tctctcgaac
     1561 caacgagagc agtatgccgt ttactgtgtg acagagtgag agagcattag tgcagagagg
     1621 gagcgaccca aaaagaaaag agagaataac gaataacggc cagagaaatt tctcgagttt
     1681 tctttctgcc aaacaaatga cctaccacaa taaccagttt gttttgggat tctagctcct
     1741 gacagcgcat catgatggac cagatgggtg aggtggagta cgcgcccggg gagcccaagg
     1801 gcacgccctg gcacccgcac cgcggcttcg agacagtgac ctacatcgtc gaccaattcc
     1861 tccctgggat ccggagagag accctatgcg gcatcagagc agattgtact gagagtgcac
     1921 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgtcat
     1981 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     2041 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     2101 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagggc
     2161 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgag
     2221 tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatgatctg cctcctgggt
     2281 ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg attaattgtc
     2341 aacaggggtc tctcgctgat ccgttaaaat tgtaagcgtt aatattttgt taaaattcgc
     2401 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     2461 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     2521 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcgc
     2581 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     2641 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     2701 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     2761 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     2821 gtaacatgaa gttttaaatc aatctaaagt atatatgtgt aacattggtc tagtgattat
     2881 ttgccgacta ccttggtgat ctcgcctttc acgtagtgga caaattcttc caactgatct
     2941 gcgcgcgagg ccaaacgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg
     3001 acgggctgat actgggccgg caggcgctcc attgcccagt cggcagctac atccttcggc
     3061 gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc
     3121 tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca
     3181 aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca
     3241 acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc
     3301 tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta
     3361 gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg
     3421 agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc
     3481 cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc actgtgtggc
     3541 ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga
     3601 tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct
     3661 tccctcataa tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     3721 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     3781 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     3841 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     3901 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     3961 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
     4021 gtcacaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     4201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     4261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     4321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     4381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     4441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     4501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     4561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     4621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4801 cccctgattc tgtggataac cgtgtcaagc gcagctgccc gggtctagag tcgacgcatg
     4861 cctcctgaca gcggttctcc ttgttctcca cggtcttaag cttagcacga gcgatcgaga
     4921 gagatagaga gcgagataga gcgaagcttg ttaattcgtc gagcgcttgc catactgaaa
     4981 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     5041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     5161 tcatgtctgg atcaagctca ctccttgcga cgagcgcgca ggcgcagagc cagataggcc
     5221 agcacgcgga aggacacaat caggatggcc aggcccacat aatccagtgg caggtcggcg
     5281 gcactgaaat tcagtgtctc caggatgacc tttccgctgc tggggcaggt cgtgttgctg
     5341 ctggtgcagg agatctcgcc gggctccaca tcggcccact gattgatcag caggccctca
     5401 ttggcgtagc gaaaccagct caggtagctc agccacttca ggtacacggg cacgctgccg
     5461 gagttcagaa agaatccgcc gaacagcagg aaggggataa tcacgggggg tcccacgctc
     5521 agggccatgc tggtgctgct ggaggcgcag ctgatcaggt agccgaagct ggtcgacacg
     5581 ttggccacca gggtcaccag ggccaggcaa ttaaagaaat gcagcactcc ggcacgcagg
     5641 ccaatcatgg ggtaggcaat ggcggtaaac accaggggca cggtcaggaa cagcggcagc
     5701 tcggcaatgg tctttcccag gaagtaggta tcgcagcggt acaggcggct gcgggcctcg
     5761 cgcatgaaca cgggcagctc gctggtgaac acgttgatgg tggcgaacac gttctggaac
     5821 gtcatgttcg tcagaaacag aaaaatggct ccattgatgt tcatcactcc gacctgcgtc
     5881 agctgctgtc ccaggaagat caggccgatc agaatggcca ccatcgtggt ctggatcagg
     5941 cggaccttga ccagcagggg ctccttcagc acactcagcc agctgcgcca cagcacggcg
     6001 cgaaactgca taaaccaggt agccttatac gtatagccgt tctcgggctg ctccaggggc
     6061 ttctccaggt tcttcgtggc cagcagctgc tccatgtcgc gggccacctt ggagatggcg
     6121 aagttatcgc agatcttggc aatgcgatcg cggctctcaa tctcgcgtcc tggcaccacg
     6181 gccagcactt gcacatagaa gtcggcgggg ttgtaattcg tggggcactg ggcgcccaca
     6241 tagctgaaga aatccacggc ctcgcttggg gttcccagga aggccacgcg tccctcggcc
     6301 atcagcagaa tcttatcaaa cagctcgaac agctccgagc tgggctggtg gatcgtcaga
     6361 atgaccgtct ttcccttctg gctcagcttc ttcagcacct gcaccacgga gtgggcggtg
     6421 aagctatcca ggccgctggt cggctcgtcg caaatcagca gggggggatc ggtcagggcc
     6481 tcgctggcaa aggccaggcg cttgcgctct ccgccactca ggcccttcac gcggcctggc
     6541 acgccaataa tggtgtgctg gcacttgctc aggctcagct cctggatgac ctggtccacg
     6601 cgggccacgc gctgacggta tgtcaggtgg cggggcatgc ggaccatggc ctgaaagatc
     6661 agatgctcgc gggctgtcag gctgccgatg aacagatcgt cctgctgcac gtaggcgcag
     6721 cgggcctgca tctccttggc atccacgggc tggccgttca gcaggcgcat gccgcttggg
     6781 gacacctgaa tgccctgggg gctgcggaag gccagggcgt tcagcagcgt ggtctttccg
     6841 gcgccactac ttcccatcac ggccagcagc tcgccggggt aggccactcc gcacacattc
     6901 ttcagcaggt gcttgcgtgg ggctgg
//
