LOCUS       sequence-328791-        6351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Basic transgenesis Mini-white physical eye marker cassette in 
            Alpha2, lacks attB site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 6351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: pMiniWhiteA2
FEATURES             Location/Qualifiers
     source          1..6351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(990..1578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1070..1089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1693..1795
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1796..2611
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1796..2611
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1869..1888)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2725..3180
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2812..2831)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3022..3043
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3220..3238)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(3690..3761,4163..4436,4511..5165,5227..5542,5746..5877,
                     5948..6351,1..211)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
ORIGIN
        1 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
       61 gtgccccagt tcgggcaagg tcatcctgga gacacttaac ttctccgccg ccgatctgcc
      121 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
      181 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
      241 tttttttttt accattatta ccatcgtgtt tactgtttat tgccccctca aaaagctaat
      301 gtaattatat ttgtgccaat aaaaacaaga tatgacctat agaatacaag tatttcccct
      361 tcgaacatcc ccacaagtag actttggatt tgtcttctaa ccaaaagact tacacacctg
      421 cataccttac atcaaaaact cgtttatcgc tacataaaac accgggatat attttttata
      481 tacatacttt tcaaatcgcg cgccctcttc ataattcacc tccaccacac cacgtttcgt
      541 agttgctctt tcgctgtctc ccacccgctc tccgcaacac attcaccttt tgttcgacga
      601 ccttggagcg actgtcgtta gttccgcgcg attcggttcg ctcaaatggt tccgagtggt
      661 tcatttcggc tcaatagaaa ttagtaataa atatttgtat gtacaattta tttgctccaa
      721 tatatttgta tatatttccc tcacagctat atttattcta atttaatatt atgacttttt
      781 aaggtaattt tttgtgacct gttcggagtg attagcgtta caatttgaac tgaaagtgac
      841 atccagtgtt tgttccttgt gtagatgcat cgcttgagac gaagcttgac acggttatcc
      901 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
      961 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1021 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1081 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1141 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1201 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1261 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1321 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1381 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     1441 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1501 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1561 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1621 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1681 atccttttgt gatgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
     1741 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
     1801 ccatattcaa cgggaaacgt cttgctcaag gccgcgatta aattccaaca tggatgctga
     1861 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctaccg
     1921 attgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     1981 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     2041 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     2101 agggaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     2161 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     2221 cagcgatcgc gtatttcgac tcgctcaggc gcaatcacga atgaataacg gtttggttga
     2281 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     2341 gcataaactt ttgccattct caccggattc agtagtcact catggtgatt tctcacttga
     2401 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     2461 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     2521 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     2581 gtttcatttg atgctcgatg agtttttctg atttttttta aggcagttat tggtgccctt
     2641 aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga
     2701 tgataagctg tcaaacatga tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     2761 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     2821 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     2881 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     2941 agggtgatgg ttcacgtagt gggccagcgc cctgatagac ggtttttcgc cctttgacgt
     3001 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3061 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3121 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3181 taacaagctt cgtctcagtc aggaggaatt ctagtatgta tgtaagttaa taaaacccat
     3241 ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa
     3301 cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt
     3361 cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc
     3421 tgtccacaga aatatcgccg tcactttcgc cgctgcgtcc gctatctctt tcgccaccgt
     3481 ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga
     3541 cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct
     3601 cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta
     3661 agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc
     3721 gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat
     3781 acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg
     3841 tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa
     3901 acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat
     3961 caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc
     4021 agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt
     4081 aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat
     4141 ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca
     4201 gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc
     4261 cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat
     4321 ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg
     4381 actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga
     4441 gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt
     4501 tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc
     4561 ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa
     4621 gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag
     4681 gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa
     4741 cacctgattt tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg
     4801 gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc
     4861 ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc
     4921 tccgaggctc taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac
     4981 tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc
     5041 gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt
     5101 ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt
     5161 tcctagtgag ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct
     5221 atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt
     5281 gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga
     5341 caattttgcc attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct
     5401 ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca
     5461 gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa
     5521 agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta
     5581 caattcttgg aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct
     5641 tagttttttt caatgagatg tatagtttat agttttgcag aagataaata aatttcattt
     5701 aactcgcgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg
     5761 attggcctca tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga
     5821 gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta
     5881 agtcatgttt agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc
     5941 gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta
     6001 tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt
     6061 gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca
     6121 cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata
     6181 tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat
     6241 cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct
     6301 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc t
//
