LOCUS       sequence-328873-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of N-terminally 6His tagged cDAXX-0SB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, 
            Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA,
            Mittag T
  TITLE     Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation 
            of Active, Phase-Separated Compartments.
  JOURNAL   Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 
            10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20.
  PUBMED    30244836
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08.027"; journalName: "Mol Cell"; date: 
            "2018-10-4- 4"; volume: "72"; issue: "1"; pages: "19-36.e8"
COMMENT     Sequence Label: pDEST17-cDAXX-0SB
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaaa acctgtattt tcagggcagc agccccatga gcagcctgca gatcagcaac
      121 gagaagaacc tggaacccgg caagcagagc cccagatcca tcggcgagca gcagaacaag
      181 ggccggatcg tgtctcccag cctgctgagc gaagaacccc tggcccctag ctccatcgac
      241 gccgagtcta acggcgaaca gcccgaggaa ctgaccctgg aagaggaatc ccccgtgtcc
      301 cagctgttcg agctggaaat cgaggccctg cccctggaca cacccagcag cgtggaaaca
      361 gacagccccc ggtccatcaa gcagtccgag gaacccttca ccaccgtgct ggaaaacggc
      421 gctggcatgg tcaccagcac caccttcaat ggcggcgtgt ccccccacaa ctggggcgat
      481 tctggccccc ctagcaagaa gtcccggaaa gagaagaagc agaccggcag cggccctctg
      541 ggcaacagct acgtggaacg gcagagaagc gtgcacgaga aaaacggcaa gaagatcagc
      601 accctgccca gccccccaag ccctctggct tctctggctc ctgtgtcccc cgatagcgcc
      661 agagtggata gccctagcca cggcctgagc ccagtgtccc tgtctattcc cagccccgcc
      721 agactgagcc agacccctca tagccagcct cccagacctg gcaccagcaa gacaagcgtg
      781 gccacccagt gcgaccccga agagatcatc gtgctgtccg acagcgacta a
//
