LOCUS       sequence-328918-       10682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BCKDK (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10682)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BCKDK-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    264..380
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    412..432
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        432..1643
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1480..1499
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1644..1668
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2905..2929)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2931..2972
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2979..3749
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(3755..3775)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    3804..4392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3857..3877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4275..4286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4406..4905
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4441..4464)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4838..4857
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4926..5525
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4926..4945)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5422..5442
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5663..5896
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5968..6089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6005..6024)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6059..6078
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6129..6264
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6284..6303)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6285..6303)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6313..6330)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6313..6329)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6322..6344)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6471..6926
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6558..6577)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6768..6789
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6952..7056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7057..7125,7126..7917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8088..8676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8577..8596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8830..8847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8964..8985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9000..9005,9006..9023,9024..9030)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9038..9054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9043..9065
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9062..9078
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9062..9078
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9097..9117
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9099..9117
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9145..9371
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9372..9552
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9599..9724
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10217..10450
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10635..10679
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
       61 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
      121 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
      181 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
      241 ggatctcgac ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca
      301 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
      361 attacaaaat tcaaaatttt atcgtccagt gtggtggaat tctgcagata tcaactttgt
      421 atagaaaagt tggcctccgc gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac
      481 ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt cctgatcctt ccgcccggac
      541 gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt atcagcagaa
      601 ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc cagagagcgg
      661 aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc cgtggggcgg
      721 tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc cgtcgcagcc
      781 gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg
      841 gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt
      901 ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg aactgggggt
      961 tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag
     1021 gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag aacccaaggt
     1081 cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct ggggcaccat
     1141 ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc gtctgttgcg
     1201 ggggcggcag ttatggcggt gccgttgggc agtgcacccg tacctttggg agcgcgcgcc
     1261 ctcgtcgtgt cgtgacgtca cccgttctgt tggcttataa tgcagggtgg ggccacctgc
     1321 cggtaggtgt gcggtaggct tttctccgtc gcaggacgca gggttcgggc ctagggtagg
     1381 ctctcctgaa tcgacaggcg ccggacctct ggtgagggga gggataagtg aggcgtcagt
     1441 ttctttggtc ggttttatgt acctatcttc ttaagtagct gaagctccgg ttttgaacta
     1501 tgcgctcggg gttggcgagt gtgttttgtg aagtttttta ggcacctttt gaaatgtaat
     1561 catttgggtc aatatgtaat tttcagtgtt agactagtaa attgtccgct aaattctggc
     1621 cgtttttggc ttttttgtta gacacaagtt tgtacaaaaa agttggcatg atcctggcgt
     1681 cggtgctgag gagcggtccc gggggcgggc ttccgctccg gcccctcctg ggacccgcac
     1741 tcgcgctccg ggcccgctcg acgtcggcca ccgacacaca ccacgtggag atggctcggg
     1801 agcgctccaa gaccgtcacc tccttttaca accagtcggc catcgacgcg gcagcggaga
     1861 agccctcagt ccgcctaacg cccaccatga tgctctacgc tggccgctct caggacggca
     1921 gccaccttct gaaaagtgct cggtacctgc agcaagaact tccagtgagg attgctcacc
     1981 gcatcaaggg cttccgctgc cttcctttca tcattggctg caaccccacc atactgcacg
     2041 tgcatgagct atatatccgt gccttccaga agctgacaga cttccctccg atcaaggacc
     2101 aggcggacga ggcccagtac tgccagctgg tgcgacagct gctggatgac cacaaggatg
     2161 tggtgaccct cttggcagag ggcctacgtg agagccggaa gcacatagag gatgaaaagc
     2221 tcgtccgcta cttcttggac aagacgctga cttcgaggct tggaatccgc atgttggcca
     2281 cgcatcacct ggcgctgcat gaggacaagc ctgactttgt cggcatcatc tgtactcgtc
     2341 tctcaccaaa gaagattatt gagaagtggg tggactttgc cagacgcctg tgtgagcaca
     2401 agtatggcaa tgcgccccgt gtccgcatca atggccatgt ggctgcccgg ttccccttca
     2461 tccctatgcc actggactac atcctgccgg agctgctcaa gaatgccatg agagccacaa
     2521 tggagagtca cctagacact ccctacaatg tcccagatgt ggtcatcacc atcgccaaca
     2581 atgatgtcga tctgatcatc aggatctcag accgtggtgg aggaatcgct cacaaagatc
     2641 tggaccgggt catggactac cacttcacta ctgctgaggc cagcacacag gacccccgga
     2701 tcagccccct ctttggccat ctggacatgc atagtggcgc ccagtcagga cccatgcacg
     2761 gctttggctt cgggttgccc acgtcacggg cctacgcgga gtacctcggt gggtctctgc
     2821 agctgcagtc cctgcagggc attggcacgg acgtctacct gcggctccgc cacatcgatg
     2881 gccgggagga aagcttccgg atctgcccaa ctttcttgta caaagtgggc ggcaagccca
     2941 tccccaaccc cctgctgggc ctggacagca ccgctagcat cccgctgctg aacgctaaac
     3001 agattctggg acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca
     3061 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
     3121 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
     3181 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
     3241 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
     3301 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
     3361 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
     3421 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
     3481 acctggacag gaatactctg gccgctatgc tgatccgaga gctgcgggca gccctggaac
     3541 tgttcgagca ggaaggcctg gctccatatc tgtcacggtg ggagaagctg gataacttca
     3601 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
     3661 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg
     3721 gagaaatcag tctgcggtct gccgaaaagt aagccaactt tattatacat agttgatatc
     3781 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     3841 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3901 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3961 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4021 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4081 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4141 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4201 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4261 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4321 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4381 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     4441 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     4501 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     4561 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     4621 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     4681 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     4741 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     4801 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     4861 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     4921 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     4981 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     5041 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     5101 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     5161 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     5221 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     5281 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     5341 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     5401 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     5461 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     5521 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     5581 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5641 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     5701 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     5761 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     5821 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     5881 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     5941 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6001 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6061 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6121 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6181 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6241 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6301 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6361 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6421 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6481 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6541 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6601 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     6661 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     6721 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     6781 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     6841 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     6901 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     6961 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7021 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7081 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7141 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7201 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7261 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7321 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7381 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7441 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7501 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7561 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7621 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     7681 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     7741 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     7801 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     7861 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     7921 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     7981 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8041 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8101 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8161 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8221 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8281 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8341 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8401 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8461 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggtggac
     8521 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8581 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     8641 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     8701 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     8761 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     8821 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     8881 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     8941 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9001 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9061 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     9121 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     9181 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     9241 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     9301 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     9361 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9541 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     9601 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     9661 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     9721 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     9781 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     9841 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     9901 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     9961 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
    10021 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
    10081 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
    10141 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
    10201 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
    10261 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
    10321 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
    10381 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
    10441 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
    10501 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
    10561 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
    10621 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
    10681 gg
//
