LOCUS       sequence-32894-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Lowary PT, Widom J
  TITLE     New DNA sequence rules for high affinity binding to histone octamer 
            and sequence-directed nucleosome positioning.
  JOURNAL   J Mol Biol. 1998 Feb 13. 276(1):19-42.
  PUBMED    9514715
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            1998 Feb 13. 276(1):19-42."
COMMENT     Sequence Label: pGEM-3z/607
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(358..376)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(359..376)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(394..410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(394..410)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(407..429)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(442..448,449..466,467..472))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    487..508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(625..642)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(876..895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgactactat gggcgaattc gagctcggta cccggggatc ctctagagtc cgggatccta
       61 atgaccaagg cctttctaat aggcatcctc gccttagggc taggaaccgg cagtagcact
      121 ggactaacca gggtccgaac ggtgcccgta tctcgggggg gggaaagatg ttcctttgcc
      181 cctctaaacc acgctaacgg gtactagaac ccgttccgga taccaaggcg ctcgttaggc
      241 ctccgggtgt tttatgcctt cccgtctcca aaacactcac ctacgcattc tccgccaaag
      301 ggtgccagtc ggatagtgtt ccgagctccc gacctgcagg catgcaagct tgagtattct
      361 atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
      421 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      481 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
      541 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      601 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      661 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      721 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      781 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      841 ctcaagtcag aagtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      901 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accgga
//
