LOCUS       sequence-329140-       10500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inducing predictable deletions in protoplasts and wheat plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10500)
  AUTHORS   Wang S, Zong Y, Lin Q, Zhang H, Chai Z, Zhang D, Chen K, Qiu JL, Gao
            C
  TITLE     Precise, predictable multi-nucleotide deletions in rice and wheat 
            using APOBEC-Cas9.
  JOURNAL   Nat Biotechnol. 2020 Dec;38(12):1460-1465. doi: 
            10.1038/s41587-020-0566-4. Epub 2020 Jun 29.
  PUBMED    32601432
REFERENCE   2  (bases 1 to 10500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0566-4"; journalName: "Nat Biotechnol"; date: 
            "2020-12"; volume: "38"; issue: "12"; pages: "1460-1465"
COMMENT     Sequence Label: pJIT163-AFID-2
FEATURES             Location/Qualifiers
     source          1..10500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1453..5553
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1453..5553
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5554..5601
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6307..6327
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6385..6561
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7115..7131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7115..7131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7128..7150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7163..7169,7170..7187,7188..7193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7346..7363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7517..8105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7597..7616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8276..9067,9068..9136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8899..8918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9137..9241)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
       61 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
      121 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
      181 aattaattct gtttcaaact acctggtgga tttattaatt ttggatctgt atgtgtgtgc
      241 catacatatt catagttacg aattgaagat gatggatgga aatatcgatc taggataggt
      301 atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt tcgcttggtt
      361 gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttct agatcggagt agaatactgt
      421 ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca tacatcttca
      481 tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca tgttgatgtg
      541 ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg ctctaacctt
      601 gagtacctat ctattataat aaacaagtat gttttataat tattttgatc ttgatatact
      661 tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct tcatacgcta
      721 tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg tgttacttct
      781 gcaaagcttg tcgacggatc catggaggcc agcccggcta gcggcccaag gcatctcatg
      841 gacccgcaca tcttcaccag caacttcaac aacggcatcg gcaggcacaa gacctacttg
      901 tgctacgagg tggagaggct cgacaacgga acctccgtga agatggacca acacaggggg
      961 ttcctccaca accaagccaa gaacctcctc tgcggcttct acggcaggca cgccgagttg
     1021 aggttcctcg acttggtgcc atccctccaa ctcgatccag cccaaatcta ccgcgtgacc
     1081 tggttcatct cctggtcccc atgcttctcc tggggttgcg ccggcgaggt tcgggctttc
     1141 ctccaagaaa acacccacgt ccgcctccgc attttcgccg ccaggatcta tgattacgac
     1201 cctctctaca aggaggccct ccagatgctg cgggacgccg gtgctcaggt gagtatcatg
     1261 acctacgacg agttcaagca ctgctgggac accttcgttg accaccaggg ctgcccattc
     1321 caaccatggg acggtctgga tgaacacagc caagccttgt ccggcaggct ccgggccatc
     1381 ctccaaaacc aggggaactc cgggagcgag acgccaggca cctccgagtc ggccacccca
     1441 gaatctctta aggacaagaa gtactcgatc ggcctcgaca tcgggacgaa ctcagttggc
     1501 tgggccgtga tcaccgacga gtacaaggtg ccctctaaga agttcaaggt cctggggaac
     1561 accgaccgcc attccatcaa gaagaacctc atcggcgctc tcctgttcga cagcggggag
     1621 accgctgagg ctacgaggct caagagaacc gctaggcgcc ggtacacgag aaggaagaac
     1681 aggatctgct acctccaaga gattttctcc aacgagatgg ccaaggttga cgattcattc
     1741 ttccaccgcc tggaggagtc tttcctcgtg gaggaggata agaagcacga gcggcatccc
     1801 atcttcggca acatcgtgga cgaggttgcc taccacgaga agtaccctac gatctaccat
     1861 ctgcggaaga agctcgtgga ctccaccgat aaggcggacc tcagactgat ctacctcgct
     1921 ctggcccaca tgatcaagtt ccgcggccat ttcctgatcg agggggatct caacccagac
     1981 aacagcgatg ttgacaagct gttcatccaa ctcgtgcaga cctacaacca actcttcgag
     2041 gagaacccga tcaacgcctc tggcgtggac gcgaaggcta tcctgtccgc gaggctctcg
     2101 aagtccagga ggctggagaa cctgatcgct cagctcccag gcgagaagaa gaacggcctg
     2161 ttcgggaacc tcatcgctct cagcctgggg ctcaccccga acttcaagtc gaacttcgat
     2221 ctcgctgagg acgccaagct gcaactctcc aaggacacct acgacgatga cctcgataac
     2281 ctcctggccc agatcggcga tcaatacgcg gacctgttcc tcgctgccaa gaacctgtcg
     2341 gacgccatcc tcctgtcaga tatcctccgc gtgaacaccg agatcacgaa ggctccactc
     2401 tctgcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct cctgaaggcg
     2461 ctggtccgcc aacagctccc ggagaagtac aaggagattt tcttcgatca gtcgaagaac
     2521 ggctacgctg ggtacatcga cggcggggcc tcacaagagg agttctacaa gttcatcaag
     2581 ccaatcctgg agaagatgga cggcacggag gagctcctgg tgaagctcaa cagggaggac
     2641 ctcctgcgga agcagagaac cttcgataac ggcagcatcc cccaccaaat ccatctcggg
     2701 gagctgcacg ccatcctgag aaggcaagag gacttctacc ctttcctcaa ggataaccgg
     2761 gagaagatcg agaagatcct gaccttcaga atcccatact acgtcggccc tctcgcgcgg
     2821 gggaactcaa gattcgcttg gatgacccgc aagtctgagg agaccatcac gccgtggaac
     2881 ttcgaggagg tggtggacaa gggcgctagc gctcagtcgt tcatcgagag gatgaccaac
     2941 ttcgacaaga acctgcccaa cgagaaggtg ctccctaagc actcgctcct gtacgagtac
     3001 ttcaccgtct acaacgagct cacgaaggtg aagtacgtca ccgagggcat gcgcaagcca
     3061 gcgttcctgt ccggggagca gaagaaggct atcgtggacc tcctgttcaa gaccaaccgg
     3121 aaggtcacgg ttaagcaact caaggaggac tacttcaaga agatcgagtg cttcgattcg
     3181 gtcgagatca gcggcgttga ggaccgcttc aacgccagcc tcgggaccta ccacgatctc
     3241 ctgaagatca tcaaggataa ggacttcctg gacaacgagg agaacgagga tatcctggag
     3301 gacatcgtgc tgaccctcac gctgttcgag gacagggaga tgatcgagga gcgcctgaag
     3361 acgtacgccc atctcttcga tgacaaggtc atgaagcaac tcaagcgccg gagatacacc
     3421 ggctggggga ggctgtcccg caagctcatc aacggcatcc gggacaagca gtccgggaag
     3481 accatcctcg acttcctcaa gagcgatggc ttcgccaaca ggaacttcat gcaactgatc
     3541 cacgatgaca gcctcacctt caaggaggat atccaaaagg ctcaagtgag cggccagggg
     3601 gactcgctgc acgagcatat cgcgaacctc gctggctccc ccgcgatcaa gaagggcatc
     3661 ctccagaccg tgaaggttgt ggacgagctc gtgaaggtca tgggccggca caagcctgag
     3721 aacatcgtca tcgagatggc cagagagaac caaaccacgc agaaggggca aaagaactct
     3781 agggagcgca tgaagcgcat cgaggagggc atcaaggagc tggggtccca aatcctcaag
     3841 gagcacccag tggagaacac ccaactgcag aacgagaagc tctacctgta ctacctccag
     3901 aacggcaggg atatgtacgt ggaccaagag ctggatatca accgcctcag cgattacgac
     3961 gtcgatcata tcgttcccca gtctttcctg aaggatgact ccatcgacaa caaggtcctc
     4021 accaggtcgg acaagaaccg cggcaagtca gataacgttc catctgagga ggtcgttaag
     4081 aagatgaaga actactggag gcagctcctg aacgccaagc tgatcacgca aaggaagttc
     4141 gacaacctca ccaaggctga gagaggcggg ctctcagagc tggacaaggc cggcttcatc
     4201 aagcggcagc tggtcgagac cagacaaatc acgaagcacg ttgcgcaaat cctcgactct
     4261 cggatgaaca cgaagtacga tgagaacgac aagctgatca gggaggttaa ggtgatcacc
     4321 ctgaagtcta agctcgtctc cgacttcagg aaggatttcc agttctacaa ggttcgcgag
     4381 atcaacaact accaccatgc ccatgacgct tacctcaacg ctgtggtcgg caccgctctg
     4441 atcaagaagt acccaaagct ggagtccgag ttcgtgtacg gggactacaa ggtttacgat
     4501 gtgcgcaaga tgatcgccaa gtcggagcaa gagatcggca aggctaccgc caagtacttc
     4561 ttctactcaa acatcatgaa cttcttcaag accgagatca cgctggccaa cggcgagatc
     4621 cggaagagac cgctcatcga gaccaacggc gagacggggg agatcgtgtg ggacaagggc
     4681 agggatttcg cgaccgtccg caaggttctc tccatgcccc aggtgaacat cgtcaagaag
     4741 accgaggtcc aaacgggcgg gttctcaaag gagtctatcc tgcctaagcg gaacagcgac
     4801 aagctcatcg ccagaaagaa ggactgggac ccaaagaagt acggcgggtt cgacagccct
     4861 accgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc caagaagctc
     4921 aagagcgtga aggagctcct ggggatcacc atcatggaga ggtccagctt cgagaagaac
     4981 ccaatcgact tcctggaggc caagggctac aaggaggtga agaaggacct gatcatcaag
     5041 ctcccgaagt actctctctt cgagctggag aacggcagga agagaatgct ggcttccgct
     5101 ggcgagctcc agaaggggaa cgagctcgcg ctgccaagca agtacgtgaa cttcctctac
     5161 ctggcttccc actacgagaa gctcaagggc agcccggagg acaacgagca aaagcagctg
     5221 ttcgtcgagc agcacaagca ttacctcgac gagatcatcg agcaaatctc cgagttcagc
     5281 aagcgcgtga tcctcgccga cgcgaacctg gataaggtcc tctccgccta caacaagcac
     5341 cgggacaagc ccatcagaga gcaagcggag aacatcatcc atctcttcac cctgacgaac
     5401 ctcggcgctc ctgctgcttt caagtacttc gacaccacga tcgatcggaa gagatacacc
     5461 tccacgaagg aggtcctgga cgcgaccctc atccaccagt cgatcaccgg cctgtacgag
     5521 acgaggatcg acctctcaca actcggcggg gataagagac ccgcagcaac caagaaggca
     5581 gggcaagcaa agaagaagaa gacgcgtgac tccggcggca gcgccaacga gctgacctgg
     5641 cacgacgtgc tcgccgagga gaagcaacag ccatacttcc tgaacaccct ccaaaccgtg
     5701 gcttccgaga ggcagagcgg cgtcaccatc tacccaccac aaaaggacgt gttcaacgcc
     5761 ttccgcttca ccgagctggg cgacgtcaag gtggtcatcc tcggccaaga cccataccac
     5821 ggcccaggcc aggctcacgg cctcgccttc tccgtcaggc caggcatcgc catcccacca
     5881 agcctcctga acatgtacaa ggagctggag aacaccatcc caggcttcac caggccaaac
     5941 cacggctacc tcgagtcctg ggctaggcaa ggcgtgctcc tgctcaacac cgtgctgacc
     6001 gtcagggctg gccaggctca ctcccacgcc agcctgggct gggagacgtt caccgacaag
     6061 gtcatctccc tcatcaacca gcacagggag ggcgtggtgt tcctcctctg gggcagccac
     6121 gcccaaaaga agggcgccat catcgacaag cagaggcacc acgtgctgaa ggccccacac
     6181 ccgtccccac tctccgctca caggggcttc ttcggctgca accacttcgt gctggccaac
     6241 caatggctcg agcagagggg cgagacgcca atcgactgga tgccagtcct cccagccgag
     6301 agcgagccaa agaagaagcg gaaggtgtag gaattcggta cgctgaaatc accagtctct
     6361 ctctacaaat ctatctctct ctattttctc cataaataat gtgtgagtag tttcccgata
     6421 agggaaatta gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag
     6481 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
     6541 tccagtacta aaatccagat ctcctaaagt ccctatagat ctttgtcgtg aatataaacc
     6601 agacacgaga cgactaaacc tggagcccag acgccgttcg aagctagaag taccgcttag
     6661 gcaggaggcc gttagggaaa agatgctaag gcagggttgg ttacgttgac tcccccgtag
     6721 gtttggttta aatatgatga agtggacgga aggaaggagg aagacaagga aggataaggt
     6781 tgcaggccct gtgcaaggta agaagatgga aatttgatag aggtacgcta ctatacttat
     6841 actatacgct aagggaatgc ttgtatttat accctatacc ccctaataac cccttatcaa
     6901 tttaagaaat aatccgcata agcccccgct taaaaattgg tatcagagcc atgaataggt
     6961 ctatgaccaa aactcaagag gataaaacct caccaaaata cgaaagagtt cttaactcta
     7021 aagataaaag atctttcaag atcaaaacta gttccctcac accggtgacg gggatcgcat
     7081 gcgatatctc gagatctagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9241 gcacatttcc ccgaaaagtg ccacctgcca gtgccaagct aattcgagct cggtacctga
     9301 cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat aaaaaattac
     9361 cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta tacatatatt
     9421 taaactttac tctacgaata atataatcta tagtactaca ataatatcag tgttttagag
     9481 aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt tgacaacagg
     9541 actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc aaatagcttc
     9601 acctatataa tacttcatcc attttattag tacatccatt tagggtttag ggttaatggt
     9661 ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc taaattaaga
     9721 aaactaaaac tctattttag tttttttatt taataattta gatataaaat agaataaaat
     9781 aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa ggaaacattt
     9841 ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc taacggacac
     9901 caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac ggcatctctg
     9961 tcgctgcctc tggacccctc tcgatcgaga gttccgctcc accgttggac ttgctccgct
    10021 gtcggcatcc agaaattgcg tggcggagcg gcagacgtga gccggcacgg caggcggcct
    10081 cctcctcctc tcacggcacc ggcagctacg ggggattcct ttcccaccgc tccttcgctt
    10141 tcccttcctc gcccgccgta ataaatagac accccctcca caccctcttt ccccaacctc
    10201 gtgttgttcg gagcgcacac acacacaacc agatctcccc caaatccacc cgtcggcacc
    10261 tccgcttcaa ggtacgccgc tcgtcctccc ccccccccct ctctaccttc tctagatcgg
    10321 cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg tgttagatcc
    10381 gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg atgcgacctg tacgtcagac
    10441 acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat ggctctagcc
//
