LOCUS       sequence-32924-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Smith K, Youngman P
  TITLE     Use of a new integrational vector to investigate 
            compartment-specific expression of the Bacillus subtilis spoIIM 
            gene.
  JOURNAL   Biochimie.   . 74(7-8):705-11.
  PUBMED    1391050
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochimie. 
             . 74(7-8):705-11."
COMMENT     Sequence Label: pKSV7
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(32..48)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(32..48)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(45..67)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    56..72
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(80..86,87..104,105..110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    125..146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ccccgggtac cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
       61 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
      121 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
      181 gaaacctgtc gtgccagctg gactaaaagg catgcaattt cataatcaaa gagagcgaaa
      241 aagtagaacg aatgatgata ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa
      301 tgataaaaat attgattaca acgaacgtgt caaagaaatt attgaatcac aaaaaacagg
      361 tacaagaaaa acgaggaaag atgctgttct tgtaaatgag ttgctagtaa catctgaccg
      421 agattttttt gagcaactgg atcagtacaa gaaagatact gtatttcata aacaggaact
      481 gcaagaagtt aaggatgagt tacagaaggc aaataagcag ttacagagtg gaatagagca
      541 tatgaggtct acgaaaccct ttgattatga aaatgagcgt acaggtttgt tctctggacg
      601 tgaagagact ggtagaaaga tattaactgc tgatgaattt gaacgcctgc aagaaacaat
      661 ctcttcgaac ggantgttga tgattacgaa aatattaaga gcacagacta ttacacagaa
      721 aatcaagaat taaaaaaacg tagagagagt ttgaaagaag tagtgaatac atggaaagag
      781 gggtatcacg aaaaaagtaa agaggttaat aaattaaagc gagagaatga tagtttgaat
      841 gagcagttga atgtatcaga gaaatttcaa gatagtacag tgactttata tcgtgctgcg
      901 ag
//
