LOCUS       sequence-329294-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pDONR221 for Gateway shuttling. Contains the CARD8 FIIND 
            (L162M-P434, no stop codon) with an interface III mutation (L368G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, 
            Bachovchin DA, Wu H
  TITLE     Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome 
            formation by sequestering its active C-terminal fragment.
  JOURNAL   Immunity. 2021 Jul 13;54(7):1392-1404.e10. doi: 
            10.1016/j.immuni.2021.04.024. Epub 2021 May 20.
  PUBMED    34019797
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2021.04.024"; journalName: "Immunity"; date: 
            "2021-07-13- 13"; volume: "54"; issue: "7"; pages: "1392-1404.e10"
COMMENT     Sequence Label: pDONR221 CARD8 FIIND L368G
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tttctggggc ctgaaggaaa tgtggatgtt gagttgattg ataagagcac aaacagatac
       61 agcgtttggt tccccactgc tggctggtat ctgtggtcag ccacaggcct cggcttcctg
      121 gtaagggatg aggtcacagt gacgattgcg tttggttcct ggagtcagca cctggccctg
      181 gacctgcagc accatgaaca gtggctggtg ggcggcccct tgtttgatgt cactgcagag
      241 ccagaggagg ctgtcgccga aatccacctc ccccacttca tctccctcca agcaggtgag
      301 gtggacgtct cctggtttct cgttgcccat tttaagaatg aagggatggt cctggagcat
      361 ccagcccggg tggagccttt ctatgctgtc ctggaaagcc ccagcttctc tctgatgggc
      421 atcctgctgc ggatcgccag tgggactcgc ctctccatcc ccatcacttc caacacattg
      481 atctattatc acccccaccc cgaagatatt aagttccact tgtaccttgt ccccagcgac
      541 gccttgctaa caaaggcgat agatgatgag gaagatcgct tccatggtgt gcgcctgcag
      601 acttcgcccc caatggaacc cgggaacttt ggttccagtt atattgtgtc taattctgct
      661 aacctgaaag taatgcccaa ggagttgaaa ttgtcctaca ggagccctgg agaaattcag
      721 cacttctcaa aattctatgc tgggcagatg aaggaaccca ttcaacttga gattactgaa
      781 aaaagacatg ggactttggt gtgggatact gaggtgaagc ca
//
