LOCUS       sequence-329513-        9859 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCerulean3 fused to the N-terminus of Bcl-2 family protein, 
            Mcl-1, in a plasmid compatable for lentiviral infection. Used to 
            make stable BMK-DKO cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, 
            Fang Q, Andrews DW
  TITLE     Efficacy and specificity of inhibitors of BCL-2 family protein 
            interactions assessed by affinity measurements in live cells.
  JOURNAL   Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. 
            Epub 2022 Apr 20.
  PUBMED    35442739
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-04-22"; volume: "8(16):eabm7375. doi"; pages: " 
            10.1126/sciadv.abm7375. Epub 2022 Apr 20"
COMMENT     Sequence Label: mCerulean3-Mcl-1-pLVX
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..315
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        316..519
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     470..490
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     516..540
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(657..659,660..662,663..1373)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(702..723)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(963..982)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1310..1331
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        2506..3005
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2541..2564)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2938..2957
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3026..3625
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3026..3045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3522..3542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3639..4227
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3692..3712)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4110..4121)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4434..5067
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5195..5211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5195..5211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5208..5230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5219..5235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5243..5249,5250..5267,5268..5273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5288..5309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5426..5443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5597..6185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5677..5696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6356..7147,7148..7216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6979..6998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7217..7321)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7369..7503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7406..7425)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7460..7479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7671..8304
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8351..8476
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8973..9206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9391..9435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9698..9813
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 tgggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
       61 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      121 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      181 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      241 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      301 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      361 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      421 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      481 ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc
      541 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccgact ctactagagg
      601 atcgctagcg ctaccggact cagatctcga gctcaagctt cgaattcgtt aacaccatgg
      661 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      721 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      781 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      841 tgaccaccct gagctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc
      901 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
      961 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1021 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1081 tggagtacaa cgccatccac ggcaacgtct atatcaccgc cgacaagcag aagaacggca
     1141 tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1201 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1261 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1321 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtccggac
     1381 tcaataacac gcgtggatcc gggggatctg gcggtacggg atcctttggc ctcaaaagaa
     1441 acgcggtaat cggactcaac ctctactgtg ggggggccgg cttgggggcc ggcagcggcg
     1501 gcgccacccg cccgggaggg cgacttttgg ctacggagaa ggaggcctcg gcccggcgag
     1561 agataggggg aggggaggcc ggcgcggtga ttggcggaag cgccggcgca agccccccgt
     1621 ccaccctcac gccagactcc cggagggtcg cgcggccgcc gcccattggc gccgaggtcc
     1681 ccgacgtcac cgcgaccccc gcgaggctgc ttttcttcgc gcccacccgc cgcgcggcgc
     1741 cgcttgagga gatggaagcc ccggccgctg acgccatcat gtcgcccgaa gaggagctgg
     1801 acgggtacga gccggagcct ctcgggaagc ggccggctgt cctgccgctg ctggagttgg
     1861 tcggggaatc tggtaataac accagtacgg acgggtcact accctcgacg ccgccgccag
     1921 cagaggagga ggaggacgag ttgtaccggc agtcgctgga gattatctct cggtaccttc
     1981 gggagcaggc caccggcgcc aaggacacaa agccaatggg caggtctggg gccaccagca
     2041 ggaaggcgct ggagacctta cgacgggttg gggatggcgt gcagcgcaac cacgagacgg
     2101 ccttccaagg catgcttcgg aaactggaca tcaaaaacga agacgatgtg aaatcgttgt
     2161 ctcgagtgat gatccatgtt ttcagcgacg gcgtaacaaa ctggggcagg attgtgactc
     2221 tcatttcttt tggtgccttt gtggctaaac acttgaagac cataaaccaa gaaagctgca
     2281 tcgaaccatt agcagaaagt atcacagacg ttctcgtaag gacaaaacgg gactggctag
     2341 ttaaacaaag aggctgggat gggtttgtgg agttcttcca tgtagaggac ctagaaggtg
     2401 gcatcaggaa tgtgctgctg gcttttgcag gtgttgctgg agtaggagct ggtttggcat
     2461 atctaataag atgaacgcgt tgaatcgatt ctagataatt ctaccgggta ggggaggcgc
     2521 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     2581 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     2641 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     2701 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     2761 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     2821 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     2881 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     2941 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     3001 tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     3061 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     3121 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     3181 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     3241 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     3301 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     3361 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     3421 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     3481 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     3541 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     3601 catgacccgc aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa
     3661 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3721 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3781 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3841 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3901 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3961 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4021 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4081 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4141 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4201 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     4261 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     4321 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     4381 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     4441 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     4501 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     4561 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     4621 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     4681 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     4741 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     4801 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     4861 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4921 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     4981 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5041 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5101 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac
     5161 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5221 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5281 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5341 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5401 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5761 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     5881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     5941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6001 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7321 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
     7381 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7501 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     7561 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     7621 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     7681 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7741 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7801 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7861 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     7921 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     7981 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     8041 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     8101 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8161 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8221 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8281 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8341 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8401 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8461 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8521 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8581 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8641 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8701 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8761 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     8821 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8881 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8941 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9001 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9061 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9121 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9181 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9241 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9301 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9361 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9421 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9481 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9541 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9601 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     9661 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
     9721 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9781 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     9841 tccagtttat cgataagct
//
