LOCUS       sequence-329602-        8829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_HSV.AU1.NWS_NGFR
FEATURES             Location/Qualifiers
     source          1..8829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      540..1128
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1029..1048
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1282..1299
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1416..1437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1452..1457,1458..1475,1476..1482)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1490..1506
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1495..1517
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1514..1530
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1514..1530
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1549..1569
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1551..1569
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1597..1823
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1824..2004
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2051..2176
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2669..2902
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3087..3131
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        3429..3677
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     3429..3449
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     3600..3619
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        3696..3771
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3819..3936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4076..5254
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4307..5245
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5202..5222
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5303..5312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5405..5437
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             5495..5518
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    6281..6869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6334..6354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6752..6763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6944..7177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7249..7370
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7286..7305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7340..7359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7410..7545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7565..7584)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7566..7584)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7594..7611)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7594..7610)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7603..7625)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7752..8207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7839..7858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8049..8070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8233..8337
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8338..8406,8407..8829,1..369)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8556..8575)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
       61 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
      121 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
      181 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
      241 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      301 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      361 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      421 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      481 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      541 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      601 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      661 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      781 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      841 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      901 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      961 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1021 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1081 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1141 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1201 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     1261 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     1321 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1381 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1441 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1501 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     1561 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     1621 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     1681 gtgcatggcg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     1741 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     1801 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     1861 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     1921 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     1981 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2041 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2101 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2161 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2221 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2281 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2341 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     2401 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     2461 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     2521 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     2581 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     2641 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     2701 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     2761 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     2821 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     2881 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     2941 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     3001 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     3061 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     3121 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     3181 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     3241 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     3301 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     3361 ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagtatcga gcggccgccc
     3421 ccttcaccga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     3481 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     3541 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     3601 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3661 ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt agagctagaa atagcaagtt
     3721 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttaa
     3781 ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     3841 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     3901 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     3961 gatccacttt ggccgcggct cgaggcgata agctttgcaa agatggataa agttttaaac
     4021 agagaggaat ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc
     4081 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4141 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4201 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     4261 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg
     4321 tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc
     4381 cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     4441 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg
     4501 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     4561 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     4621 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     4681 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     4741 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     4801 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     4861 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     4921 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     4981 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     5041 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     5101 tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     5161 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     5221 tcaaagtttt tttcttccat ttcaggtgtc gtgaggaatt ctgcagtcga tcgacggtac
     5281 cgcgggcccg ggatccacgg tcgccaccat gggggcaggt gccaccggcc gcgccatgga
     5341 cgggccgcgc ctgctgctgt tgctgcttct gggggtgtcc cttggaggtg ccaaggaggc
     5401 atgccagccc gagctggccc ccgaggaccc cgaggacgga ggaggcgggg gaggggacac
     5461 ctatcggtac attggaggag gcgggggagg gaactggtcc cacccccaat tcgagaaacc
     5521 cacaggcctg tacacacaca gcggtgagtg ctgcaaagcc tgcaacctgg gcgagggtgt
     5581 ggcccagcct tgtggagcca accagaccgt gtgtgagccc tgcctggaca gcgtgacgtt
     5641 ctccgacgtg gtgagcgcga ccgagccgtg caagccgtgc accgagtgcg tggggctcca
     5701 gagcatgtcg gcgccgtgcg tggaggccga cgacgccgtg tgccgctgcg cctacggcta
     5761 ctaccaggat gagacgactg ggcgctgcga ggcgtgccgc gtgtgcgagg cgggctcggg
     5821 cctcgtgttc tcctgccagg acaagcagaa caccgtgtgc gaggagtgcc ccgacggcac
     5881 gtattccgac gaggccaacc acgtggaccc gtgcctgccc tgcaccgtgt gcgaggacac
     5941 cgagcgccag ctccgcgagt gcacacgctg ggccgacgcc gagtgcgagg agatccctgg
     6001 ccgttggatt acacggtcca cacccccaga gggctcggac agcacagccc ccagcaccca
     6061 ggagcctgag gcacctccag aacaagacct catagccagc acggtggcag gtgtggtgac
     6121 cacagtgatg ggcagctccc agcccgtggt gacccgaggc accaccgaca acctcatccc
     6181 tgtctattgc tccatcctgg ctgctgtggt tgtgggcctt gtggcctaca tagccttcaa
     6241 gaggtggaac aggggtatcc tctagagcgg ccgcgtcgac aatcaacctc tggattacaa
     6301 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     6361 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     6421 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     6481 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     6541 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     6601 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     6661 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     6721 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     6781 ccgcggcctg ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag
     6841 tcggatctcc ctttgggccg cctccccgcc tgggtacctt taagaccaat gacttacaag
     6901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6961 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7021 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7081 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7141 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7201 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7261 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7321 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7381 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     7441 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     7501 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     7561 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     7621 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     7681 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     7741 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8161 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     8221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     8281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     8341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     8401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     8461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     8521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     8581 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     8641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     8701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     8761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     8821 cgttgggaa
//
