LOCUS       sequence-329698-        9587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: Lenti-Cry2-mCherry-coiled coil
FEATURES             Location/Qualifiers
     source          1..9587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3423..3432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3435..3455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4380..5873
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    5977..6565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6030..6050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6448..6459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6640..6873
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6992..7010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7078..7182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7183..7251,7252..8043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7401..7420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8214..8802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8703..8722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8956..8973
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9213..9234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9249..9254,9255..9272,9273..9279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9287..9303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9292..9314
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9311..9327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9311..9327
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9377..9396
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 aggccaccat ggaccccaag aagaagagga aggtgagcaa ctccaaccag cagcaacaga
     3481 tgcgactgca gcaactgcag atggagaagg agaggctgcg gctgaaacag caagaactgc
     3541 ttcggcaggc aatgcggaat atcaatccca gcacagcaaa ttctccaaaa tgtcaggagt
     3601 tagccctgcg tagccagtta ccaacactgg agcaggatgg tctcgagtct agaggatcca
     3661 tggtgtctaa aggcgaggag gataacatgg ctataatcaa ggaatttatg aggttcaagg
     3721 tgcatatgga gggatctgtg aacggtcacg agttcgaaat cgagggcgaa ggcgaggggc
     3781 gaccctatga agggacacag acggccaaac tgaaggtgac caagggtggc cccctgcctt
     3841 tcgcctggga catcctgtca ccccagttta tgtacggctc taaggcttac gttaagcacc
     3901 ctgccgatat tccggactac ttgaaactga gcttccctga agggttcaag tgggaacggg
     3961 taatgaattt tgaggatggc ggtgtagtca cagttaccca ggacagctct cttcaggacg
     4021 gagaatttat ctataaggtt aaacttcggg gcactaactt cccatcagac ggccccgtga
     4081 tgcagaagaa aactatgggc tgggaagcca gctcagaacg catgtatccc gaggacggag
     4141 cactgaaggg agaaatcaag cagcgactca agctgaagga tgggggacat tatgatgctg
     4201 aggtcaaaac cacctataag gccaagaaac ccgttcagct tcctggggca tacaacgtga
     4261 atatcaaact ggatattaca tcccataacg aagactatac catcgtggaa cagtacgagc
     4321 gggccgaggg cagacatagc acagggggca tggatgaatt gtacaaggcg gccacgcgta
     4381 tgaagatgga caaaaagact atagtttggt ttagaagaga cctaaggatt gaggataatc
     4441 ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg
     4501 aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa caatcacttg
     4561 ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc aaaacccaca
     4621 acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta
     4681 accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag aagctggtgg
     4741 aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg tgggagatat
     4801 actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa tgcttagata
     4861 tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata actgcagcgg
     4921 ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag gccgagaaac
     4981 cgagcaatgc gttgttaact agagcttggt ctccaggatg gagcaatgct gataagttac
     5041 taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag aaagttgttg
     5101 ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc gtcagacacg
     5161 ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac agtgaaggag
     5221 aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct cggtatatat
     5281 gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg tttttccctt
     5341 gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt tatccgttgg
     5401 tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga ataagagtga
     5461 ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga atgaagtatt
     5521 tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg cagtatatct
     5581 ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg ttacaaggcg
     5641 ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt gcgagattgc
     5701 caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa gcttctggtg
     5761 tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct cgtgagctac
     5821 tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca gcagcccggg
     5881 atccaccggt cgccaccggt tcagggagcg gatccggctc agggagtgcg gccgcaactc
     5941 ccacctgcaa catgcgtgac tgaatcgata gatcctaatc aacctctgga ttacaaaatt
     6001 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6061 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6121 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6181 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     6241 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     6301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     6361 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     6421 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     6481 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     6541 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
     6601 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     6661 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     6721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6841 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     6901 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
     6961 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7021 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     7081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     7141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     7201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     7261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     7321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     7381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     7441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     7501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     7561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     7621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     8041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     8101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8341 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     8401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8881 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8941 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     9001 ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt
     9061 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9121 gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc
     9181 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     9241 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     9301 caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt
     9361 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     9421 aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc
     9481 actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga attattttca
     9541 ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
