LOCUS       sequence-32974-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDRAV-3
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(25..58)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    119..152
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        180..679
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(215..238)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     612..631
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ctactatagg gcgaattgga gctcataact tcgtatagca tacattatac gaagttatgg
       61 cgcctcagcg gccgcacaat gcatgtagtt aacttcttaa ttaactggga tcctgcgggt
      121 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacctcga gattctaccg
      181 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
      241 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
      301 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
      361 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
      421 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
      481 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
      541 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
      601 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
      661 ctcatctccg ggcctttcga ccgatccagc cgccaccatg aaaaagcctg aactcaccgc
      721 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
      781 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
      841 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactt
//
