LOCUS       sequence-32980-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDREV-2
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..385)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(392..445)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(463..494,495..578)
                     /label=En2 SA
                     /note="splice acceptor from the mouse Engrailed 2 gene 
                     (Skarnes et al., 1992)"
ORIGIN
        1 ccatggtgga tttagcgctg gtgtacttgg tgacggcctt agtaccctcg gacacggcgt
       61 gcttggccaa ctccccaggc agcagcaggc gcacggccgt ctggatctcc ctggaggtga
      121 tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg cgctcgaaaa
      181 tgtcgttcac aaacgaattc atgatgccca tggccttgga cgaaatgccg gtgtcagggt
      241 ggacctgctt cagaaccttg tacacataga tggaatagct ctccttgcgg ctgcgcttgc
      301 gcttcttgcc gtctttcttc tgcgccttag tcaccgcctt cttggagccc tttttcgggg
      361 cgggagcaga cttcgctggc tctggtcccc ccggtccggg attctcctcc acgtcaccgc
      421 atgttagaag acttcctctg ccctcggaga tctgcgatct gcgttcttct tctttggttt
      481 tcgggacctg ggacctggtt gtcatggagg agaaagggca gaggttactg gttgctggag
      541 tctagctact tatccacaac caacgcaccc aagctagctt gaggttgcag atactggggg
      601 tggggggggg gatgacccgc ccaa
//
