LOCUS       sequence-32982-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Li W, You L, Cooper J, Schiavon G, Pepe-Caprio A, Zhou L, Ishii R, 
            Giovannini M, Hanemann CO, Long SB, Erdjument-Bromage H, Zhou P, 
            Tempst P, Giancotti FG
  TITLE     Merlin/NF2 suppresses tumorigenesis by inhibiting the E3 ubiquitin 
            ligase CRL4(DCAF1) in the nucleus.
  JOURNAL   Cell. 2010 Feb 19. 140(4):477-90.
  PUBMED    20178741
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Feb 19. 140(4):477-90."
COMMENT     Sequence Label: pRK5-Merlin-Flag-HA
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(43..69)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(85..108)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tattaagctc ataacagttg ggccatggcg gccaagcttc taagcgtaat ctggaacatc
       61 gtatgggtat cctccagcgg ccgacttgtc gtcatcgtct ttgtagtctc tagaggatcc
      121 agctccagct cctgcgagtt cttcaaagaa ggccactcgg gacttggcgc tctgcagagt
      181 gagcttttta atggtattat gcttgctgct ggggccgcct ctgtctgagc tctcgctgtg
      241 taggacgtcc aaggccgtct cccgctcttt gagtttcaag gcctcgatct cagtcttgag
      301 ctcgttgagc tgctcctgca gatgcttgct cttctccatg tactccactt tttctttctc
      361 tatctccatg gaaagtcgct tcatgtccgt atccttgaag tcgaatgaca agctgtcagc
      421 aataatgtcg aagctcggta tgtcaggagg cagtggtggt ggaattgggt tcatgggtgg
      481 ataggtgggc ttggtggcga tttctaagag cttctgcttg gctcttcgct ctgcttctcg
      541 ggcttcttgc aagtcttgct ttaactgatc agcctccttg gccctcctct ctgactcctc
      601 agccatcttc aatgccagca cttcagcctc cagcaccttc tgctccatca ggcgcttctc
      661 ctcctctgtc cgaatggccg tggccttgat tcgctgcatc tcttgctcag cctctgcagc
      721 cttttgtgcc aaaagcttgg cctcctcctc tgtgatctga gccttttcag ccaacagatc
      781 agctgtctcc tcagagcgca tcagagcttc attggccatc gttgcttctt ctttcatctg
      841 caggagcctc ctctctaact catctcttgt acgctcggcc tcctcccgca tctgcttctc
      901 tcgagccagc cgctgcctt
//
