LOCUS       sequence-329969-        5342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of mCherry and MOB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: mCherry-N1-MOB1
FEATURES             Location/Qualifiers
     source          1..5342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    116..237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(153..172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     207..226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(244..699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(381..402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     593..612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        726..830
                     /gene="bla"
                     /label=AmpR promoter
     promoter        832..1189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        832..1023
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(855..875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1040..1175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1224..2018
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1224..2018
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1278..1297)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1888..1907
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2206..2225)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2250..2297
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2626..3214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3115..3134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3389..3692
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3693..3896
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3847..3867
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4626..5336
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4771..4789)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5039..5058
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
       61 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
      121 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
      181 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
      241 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
      301 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      361 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
      421 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
      481 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
      541 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
      601 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
      661 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
      721 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      781 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
      841 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
      901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
      961 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     1021 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     1081 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     1141 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     1201 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     1261 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     1321 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     1381 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     1441 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     1501 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     1561 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     1621 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     1681 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     1741 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     1801 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     1861 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     1921 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     1981 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     2041 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     2101 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     2161 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     2221 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     2281 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     2341 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     2401 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     2461 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2521 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2581 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2641 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2701 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2761 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2821 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2881 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2941 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3001 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3061 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3121 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3181 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3241 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     3301 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     3361 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     3421 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     3481 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     3541 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     3601 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     3661 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     3721 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     3781 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     3841 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     3901 tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
     3961 tcatgagctt cctcttcagc agccgctctt ctaaaacatt caaaccaaag aagaacatcc
     4021 ccgaagggtc tcatcagtat gaactcttaa aacacgcaga agcaactcta ggaagtggga
     4081 atctgagaca agctgtgatg ttgccagagg gagaggacct caatgaatgg attgcagtta
     4141 acactgtgga tttcttcaac cagatcaaca tgttatatgg aaccatcacc gaattctgca
     4201 cggaagcgag ctgcccagtg atgtctgcag gtccacgata cgaatatcat tgggcagatg
     4261 gtactaatat taaaaagcca atcaaatgct ctgcaccaaa atatatcgac tatttgatga
     4321 cttgggttca ggatcagctg gatgacgaaa ctctttttcc ttctaagatt ggtgtcccgt
     4381 ttcccaaaaa cttcatgtct gtggctaaga ccattctgaa gcgtcttttc agggtgtatg
     4441 cccacatcta ccatcagcac tttgactctg tgatgcagct gcaggaggag gcccacctca
     4501 acacctcgtt taagcacttt attttctttg ttcaggagtt taatctgatt gatcggcgtg
     4561 agttggcacc tcttcaggaa ttaattgaga agcttggatc caaagacaga ggtggaggcg
     4621 ggtctatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     4681 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     4741 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     4801 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     4861 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     4921 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     4981 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     5041 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     5101 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     5161 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     5221 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     5281 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagcgg
     5341 cc
//
