LOCUS       sequence-330077-        3154 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 
            construct expression in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Kaberniuk AA, Baloban M, Monakhov MV, Shcherbakova DM, Verkhusha VV
  TITLE     Single-component near-infrared optogenetic systems for gene 
            transcription regulation.
  JOURNAL   Nat Commun. 2021 Jun 23;12(1):3859. doi: 10.1038/s41467-021-24212-7.
  PUBMED    34162879
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-23"; volume: "12(1):3859. doi"; pages: " 
            10.1038/s41467-021-24212-7"
COMMENT     Sequence Label: pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2
FEATURES             Location/Qualifiers
     source          1..3154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2140..2373
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             2392..2445
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2452..3150
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
ORIGIN
        1 atggcgcgca agaagaagag gaagtccgga ggtggtggaa agctactgtc ttctatcgaa
       61 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      121 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      181 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagactagt
      241 gctggtgggt ccgccggcgg gagtgctggc ggtagtgccg gcggtgaatt cgccgccgac
      301 ctgggctctg acgatatcag caagctgatc gccgcctgcg atcaggagcc aatccacatc
      361 cccaatgcca tccagccatt tggcgccatg ctgatcgtgg agaaggacac acagcagatc
      421 gtgtacgcct ctgccaacag cgccgagtac ttcagcgtgg ccgacaatac catccacgag
      481 ctgtccgatt tcaagcaggc caacatcaat tctctgctgc ccgagcagct gatcagcggc
      541 ctgacatccg ccatctctga gaacgagcct atctgggtgg agaccgacag gctgagcttt
      601 ctgggctggc gccacgagaa ctactatatc atcgaggtgg agagatacca cgtgcagaca
      661 tccaattggt tcgagatcca gtttcagcgg gccttccaga agctgagaaa ctgtaagacc
      721 cacaacgatc tgatcaatac cctgacacgg ctgatccagg agatcagcgg ctacgacaga
      781 gtgatgatct atcagttcga tcccgagtgg aatggcagag tgatcgccga gagcgtgaga
      841 cagctgttta cctccatgct gaaccaccac ttcccagcct ctgacatccc tgcacaggcc
      901 agggccatgt acagcatcaa cccaatccgc atcatccccg atgtgaatgc cgagccccag
      961 cctctgcaca tgatccacaa gccacagaac acagaggccg tgaatctgtc ctgcggcgtg
     1021 ctgagggccg tgtctccact gcacatgcag tatctgcgca actttggcgt gtctgccagc
     1081 acctccatcg gcatcttcaa tgaggacaag ctgtggggca tcgtggcctg tcaccacaca
     1141 aagcctaggg ccatcggccg gagaatcagg cacctgctgg tgcgcaccgt ggagtttgca
     1201 gcagagcgcc tgtggctgat ccactccagg aatgtggagc ggtacatggt gacagtgcag
     1261 gcagcccggg agcagctgtc taccacagcc gacgataagc acagctccca cgagatcgtg
     1321 atcgagcacg ccgccgactg gtgcaagctg ttccggtgtg atggcgtggg ctacctgaga
     1381 ggcgaggagc tgaccacata tggcgagacc cctgatcaga ccacaatcaa caagctggtg
     1441 gagtggctgg aggagaatgg caagaagagc ctgttttggc actcccacat gctgaaggag
     1501 gacgcacctg gactgctgcc agatggcagc cggttcgcag gactgctggc catcccactg
     1561 aagtctgacg ccgatctgtt tagctacctg ctgctgttca gggtggcaca gaacgaggtg
     1621 cgcacatggg caggcaagcc tgagaagctg tccgtggaga cctctacagg caccatggtg
     1681 ggcccacgga agtcttttga ggcctggcag gacgaggtga gcggcaagtc ccagccttgg
     1741 agaaccgcac agctgtatgc agcccgggac atcgcccggg acctgctgat cgtggccgat
     1801 agcatgcagc tgaacctgct gaatgaccag ctggccgatg ccaacgagaa tctggagaag
     1861 ctggcctcct tcgacgatct gacctctggc ggcggtacca gcgggggtgg tggatcaggt
     1921 ggaggaggtt ctggaggtgg tggatcagga ggaggtggtt ctggaggtgg tggatcagga
     1981 ggaagatctg cgtacagccg cgcgcgtacg aaaaacaatt acgggtctac catcgagggc
     2041 ctgctcgatc tcccggacga cgacgccccc gaagaggcgg ggctggcggc tccgcgcctg
     2101 tcctttctcc ccgcgggaca cacgcgcaga ctgtcgacgg cccccccgac cgatgtcagc
     2161 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
     2221 gacgatttcg atctggacat gttgggggac ggggattccc ctggtccggg atttaccccc
     2281 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt
     2341 accgatgccc ttggaattga cgagtacggt ggggctcgag ataggcgcgc cgagggcaga
     2401 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaactag tagcgagctg
     2461 attaaggaga acatgcacat gaagctgtac atggagggca ccgtggacaa ccatcacttc
     2521 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     2581 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctactag cttcctctac
     2641 ggcagcaaga ccttcatcaa ccacacccag ggcatccccg acttcttcaa gcagtccttc
     2701 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     2761 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     2821 aacttcacat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccttcacc
     2881 gagacgctgt accccgctga cggcggcctg gaaggcagaa acgacatggc cctgaagctc
     2941 gtgggcggga gccatctgat cgcaaacgcc aagaccacat atagatccaa gaaacccgct
     3001 aagaacctca agatgcctgg cgtctactat gtggactaca gactggaaag aatcaaggag
     3061 gccaacaacg agacctacgt cgagcagcac gaggtggcag tggccagata ctgcgacctc
     3121 cctagcaaac tggggcacaa gcttaattaa cccg
//
