LOCUS       sequence-330243-        8677 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-inducible Cre recombinase (LiCre) lentiviral expression 
            plasmid for light-induced Cre/Lox recombination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Duplus-Bottin H, Spichty M, Triqueneaux G, Place C, Mangeot PE, 
            Ohlmann T, Vittoz F, Yvert G
  TITLE     A single-chain and fast-responding light-inducible Cre recombinase 
            as a novel optogenetic switch.
  JOURNAL   Elife. 2021 Feb 23;10. pii: 61268. doi: 10.7554/eLife.61268.
  PUBMED    33620312
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2021-02-23"; volume: "10. pii: 61268. doi"; pages: " 
            10.7554/eLife.61268"
COMMENT     Sequence Label: pGY577
FEATURES             Location/Qualifiers
     source          1..8677
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        756..1135
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1136..1339
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        2992..3371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3372..3575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3621..3641
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3645..3665
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3678..3695
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5098..5686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5151..5171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5569..5580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        complement(6489..6507)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6528..6544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6528..6544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6541..6563)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6552..6568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6576..6582,6583..6600,6601..6606))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6621..6642
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6759..6776)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6930..7518)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7010..7029)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7689..8480,8481..8549))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8312..8331
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8550..8654)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg
      661 cggtggcggc cgctctagaa ttccattgca tacgttgtat ccatatcata atatgtacat
      721 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
      781 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      841 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      901 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      961 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     1021 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     1081 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     1141 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     1201 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     1261 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     1321 ggtctatata agcagagctc gtttagtgaa ccgcagtcgc tctgcggaga ggctggcaga
     1381 ttgagccctg ggaggttctc tccagcacta gcaggtagag cctgggtgtt ccctgctaga
     1441 ctctcaccag cacttggcca gtgctgggca gagtggctcc acgcttgctt gcttaaagac
     1501 ctcttcaata aagctgccat tttagaagta agccagtgtg tgttcccatc tctcctagtc
     1561 gccgcctggt caactcggta ctcggtaata agaagaccct ggtctgttag gaccctttct
     1621 gctttgagaa accgaagcag gaaaatccct agcagattgg cgcccgaaca gggacttgaa
     1681 ggagagtgag agactcctga gtacggctga gtgaaggcag taagggcggc aggaaccaac
     1741 cacgacggag tgctcctata aaggcgcggg tcggtaccag acggcgtgag gagcgggaga
     1801 ggaggaggcc tccggttgca ggtaagtgca acacaaaaaa gaaatagctg tcttgttatc
     1861 caggaaggga taataagata gagtgggaga tgggcgcgag aaactccgtc ttgtcaggga
     1921 agaaagcaga tgaattgaaa aaattattaa tcgcatgaat tttaaaagaa ggggaggaat
     1981 aggggatatg actccagcag aaagattaat taacatgatc actacagaac aagaaataca
     2041 atttcaacaa tcaaaaaact caaaatttaa aaattttcgg gtctattaca gagctcacgc
     2101 gtgattggag ttgggagatt ataaattagt agagatcact ccgattggct tggcccccac
     2161 agatgtgaag aggtacacta ctggtggcac ctcaagaaat aaaagagggg tctttgtgct
     2221 agggttcttg ggttttctcg caacggcagg ttctgcaatg ggcgcggcgt cgttgacgct
     2281 gaccgctcag tcccggactt tattggctgg gatagtgcag caacagcaac agctgttgga
     2341 cgtggtcaag agacaacaag aattgttgcg actgaccgtc tggggaacaa agaacctcca
     2401 gactagggtc actgccatcg agaagtactt aaaggaccag gcgcagctaa atgcttgggg
     2461 atgtgcgttt agacaagtct gccacactac tgtaccatgg ccaaatgcaa gtctaacacc
     2521 agactggaac aatgatactt ggcaagagtg ggagcgaaag gttgacttct tggaggaaaa
     2581 tataacagcc ctcctagaag aggcacaaat tcaacaagag aagaacatgt atgaattaca
     2641 aaagttgaat agctgggatg tgtttggcaa ttggtttgac cttgcttctt ggataaagta
     2701 tatacaatat ggaatttatg tagttgtagg agtaatactg ttaagaatag tgatctatat
     2761 agtacaaatg ctagctaagt taaggcaggg gtataggcca gtgttctctt ccccaccctc
     2821 ttatttccag tagactcata cccaacagga cccggcactg ccaaccagag aaggcaaaga
     2881 aggagacggt ggagaaggcg gtggatccta ttatcgatcc attgcatacg ttgtatccat
     2941 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     3001 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     3061 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     3121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     3181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     3241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     3301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     3361 ctattaccat ggtgatgcgg ttttggcagt acgtcaatgg gcgtggatag cggtttgact
     3421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     3481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     3541 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatccagc
     3601 tggctaccgg taccatgggt ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa
     3661 aggtaatgag gggcagccac caccaccacc accacggcag cctggccacc accctggaga
     3721 ggatcgagaa gaacttcgtg atcaccgacc ccaggctgcc cgacaacccc atcatcttcg
     3781 ccagcgacag cttcctgcag ctgaccgagt acagcaggga ggagatcctg ggcaggaact
     3841 gcaggttcct gcagggcccc gagaccgaca gggccaccgt gaggaagatc agggacgcca
     3901 tcgacaacca gaccgaggtg accgtgcagc tgatcaacta caccaagagc ggcaagaagt
     3961 tctggaacgt gttccacctg cagcccatga gggactacaa gggcgacgtg cagtacttca
     4021 tcggcgtgca gctggacggc accgagaggc tgcacggcgc cgccgagagg gaggccgtgt
     4081 gcctgatcaa gaagaccgcc ttccagatcg acagcgacga ggtgaggaag aacctgatgg
     4141 acatgttcag ggacaggcag gccttcagcg agcacacctg gaagatgctg ctgagcgtgt
     4201 gcaggagctg ggccgcctgg tgcaagctga acaacaggaa gtggttcccc gccgagcccg
     4261 aggacgtgag ggactacctg ctgtacctgc aggccagggg cctggccgtg aagaccatcc
     4321 agcagcacct gggccagctg aacatgctgc acaggaggag cggcctgccc aggcccagcg
     4381 acagcaacgc cgtgagcctg gtgatgagga ggatcaggaa ggagaacgtg gacgccggcg
     4441 agagggccaa gcaggccctg gccttcgaga ggaccgactt cgaccaggtg aggagcctga
     4501 tggagaacag cgacaggtgc caggacatca ggaacctggc cttcctgggc atcgcctaca
     4561 acaccctgct gaggatcgcc gagatcgcca ggatcagggt gaaggacatc agcaggaccg
     4621 acggcggcag gatgctgatc cacatcggca ggaccaagac cctggtgagc accgccggcg
     4681 tggagaaggc cctgagcctg ggcgtgacca agctggtgga gaggtggatc agcgtgagcg
     4741 gcgtggccga cgaccccaac aactacctgt tctgcagggt gaggaagaac ggcgtggccg
     4801 cccccagcgc caccagccag ctgagcacca gggccctgga gggcatcttc gaggccaccc
     4861 acaggctgat ctacggcgcc aaggacgaca gcggccagag gtacctggcc tggagcggcc
     4921 acagcgccag ggtgggcgcc gccagggaca tggccagggc cggcgtgagc atccccgaga
     4981 tcatgcaggc cggcggctgg accaacgtga acatcgtgat gaactacatc aggaacctgg
     5041 acagcgagac cggcgccatg gtgaggctgc tggccgccgg cgactaagct tggatccaat
     5101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     5161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5461 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgc
     5701 gactcgagca aactcacact caagtttatt tggcaaactc acactcaagt ttatttggca
     5761 aactcacact caagtttatg catggtcgag ttttataaaa gaaaaggggg gactggaagg
     5821 gatttattac agtgcaagaa gacatagaat cttagacatg tacttagaaa aggaagaagg
     5881 catcatacca gattggcagg attacacctc aggaccagga attagatacc caaagacatt
     5941 tggctggcta tggaaattag tccctgtaaa tgtatcagat gaggcacagg aggatgagag
     6001 gcattattta atgcagccag ctcaaacttc caagtgggat gacccttggg gagaggttct
     6061 agcgtggaag tttgatccaa ctctagccta cacttatgag gcatatgcta gatacccaga
     6121 agagttggaa gcaagtcagg cctgtcagaa ctgcatttcg ctctgtattc agtcgctctg
     6181 cggagaggct ggcagattga gccctgggag gttctctcca gcactagcag gtagagcctg
     6241 ggtgttccct gctagactct caccagcact tggccagtgc tgggcagagt ggctccacgc
     6301 ttgcttgctt aaagacctct tcaataaagc tgccatttta gaagtaagcc agtgtgtgtt
     6361 cccatctctc ctagtcgccg cctggtcaac tcggtactcg gtaataagaa gaccctggtc
     6421 tgttaggacc ctttctgctt tgagaaaccg aagcaggaaa atccctagca tggtaccagc
     6481 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt
     6541 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6601 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6661 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6721 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6781 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6841 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6901 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6961 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7021 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7081 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7141 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7201 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7261 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7321 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7381 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7441 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7501 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7561 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7621 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7681 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7741 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7801 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7861 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7921 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7981 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8041 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8101 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8161 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8221 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8281 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8341 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8401 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8461 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8521 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8581 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8641 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
