LOCUS       sequence-330439-       21246 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A kanamycin resistant plasmid for Tn7-mediated chromosomal 
            integration of Photorhabdus luminescens luxCDABE operon with Pkan 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21246)
  AUTHORS   Matsumoto A, Schluter T, Melkonian K, Takeda A, Nakagami H, Mine A
  TITLE     A versatile Tn7 transposon-based bioluminescence tagging tool for 
            quantitative and spatial detection of bacteria in plants.
  JOURNAL   Plant Commun. 2021 Jul 20;3(1):100227. doi: 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10.
  PUBMED    35059625
REFERENCE   2  (bases 1 to 21246)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2021-07-20"; volume: "3(1):100227. doi"; pages: " 
            10.1016/j.xplc.2021.100227. eCollection 2022 Jan 10"
COMMENT     Sequence Label: pBJ4
FEATURES             Location/Qualifiers
     source          1..21246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1252
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(512..531)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1122..1141
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    1657..1678
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1693..1698,1699..1716,1717..1723)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1731..1747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1755..1771
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1790..1810
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1792..1810
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(1891..1918)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2010..2096)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2249..2266
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     promoter        complement(8957..9241)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(9015..9034)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             9268..10146
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        complement(16750..16768)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(16751..16768)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(16786..16802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    16810..16826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16834..16840,16841..16858,16859..16864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16879..16900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     mobile_element  17746..17911
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(17988..18004)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17988..18004)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(18036..18055)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(18037..18055)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(18065..18081)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(18074..18096)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(18790..19452)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      19453..20224
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca tgcgcccaat
     1561 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1621 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     1681 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     1741 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1801 tcactaaagg gaacaaaagc tgggtaccgg gccgcggata catatttgaa tgtatttaga
     1861 aaaataaaca aaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc
     1921 ttaatttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc
     1981 ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac
     2041 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
     2101 ctggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc tacggcgttt
     2161 cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg ctactgccgc caggcaaatt
     2221 ctgttttatc agaccgcttc tgcgttctga tttaatctgt atcaggctga aaatcttctc
     2281 tcatccgcca aaacagccaa gcttgcatgc ctgcaggtcg actctagagg atcccctaaa
     2341 aataatgacc gagctagctc taactgtggg atgtgataac gagtcttgct atatggtgtt
     2401 tcagcttcgt aaataaatga actctgcgct ccatccttgt tgcgtacgga agcaaaggac
     2461 gagagattag gaaaatcgat tattttggct cgttgaagat ccgatgcttc aaactcaatc
     2521 acacgatccg ccttatttat ttgctttgtt gtactgttaa ccaccttagc tcgacttaat
     2581 aaaggcaaat gagtaaatcg agtccatttc cgttcttgca ttggattaaa ccaaacaaaa
     2641 atacgccact ccttatgacc cgagttacgg aataaatgac caatatgtac aacctgcaca
     2701 ttgtcattaa atgtagctag cctaaccact cactcttccc cataaaccat ttccaagaat
     2761 ctttgtgctt cctcagttat ccgctcttta gataacgttg cacttcttaa taatcgccag
     2821 cgcctaagct caacatcttc ctgtttaagc ttaataaaag cttggctaat ccgtctaatt
     2881 tgataatctt ccacactctc tgagtaacgc tttaagcaaa gcgctaccaa aggcagtttc
     2941 tgtagatttt ttgcaagaga ggttccgtta ggagtttgct ttaacagcca gctcgatgtc
     3001 gctcttgggt gatcaaggct actatctaga cgctttatga ttcttaatag ttgtcgtaca
     3061 gcaattcgat ctctttggtt ccaatcaact ctaggggcgg gtgccagacg ctcttgttga
     3121 tgctgttgat tccagtgaac tagccaatcc ctgtcatgtc ggtaaagcca agcgtatagc
     3181 accccaccct ctaaagactg tcttgccgcc ttaattcctt ggtatttatg cactagttgc
     3241 tgccagtctt tatgcttaac agataaatct tcagagttag gttgcacaga ctggctaaca
     3301 ggtcttgtcg ttatagagtg ctcagtaaga gcacttgcct gctgtagcgc ttctataacc
     3361 gttagttttg gcaataaggc ttgccacaca atactatgct gtaaataact aaaggctttt
     3421 ctatgcttac ggaatatact tttcagccaa cacgtgtcct tgttctctgc caactttaaa
     3481 tccagtttct ctagtgcctc atcactaaaa gtctgcctca ctctctccgc caccaagtca
     3541 tgacgaatgt gcttgctttt ggttagccct agatcctgcg ctaagcgctg ataaaacagc
     3601 gtccactgct caaggcttgg ggaaagctct tgcgctcgtg gagcatctaa cagaggggct
     3661 atataagcag ctagtgctgt taattgagat agggagtctt tggggtagtc tgaaagcagc
     3721 tcagtatgac ccaaagccca aaattgatgt cggtgatcat ctacagctct atcaaagaag
     3781 actaaagcac cgtgttttgg acaatatggc aaagcgggca aataccaatc tcgttgccaa
     3841 aaggcttccc catacctatt tagctgaaga gcaacgcaat cagggcagta tctaaagcgg
     3901 ttatcgctct taactctaga agcagcgact cctagcatta aatgcaccgc accttgcgct
     3961 tggtactcca ttaaccgaat agcttcgtct cggcgctcct tgcctacaaa cggagcatat
     4021 aaagggaata aggtatgctc ataaataagc tgctgaacag cgtaacgtcc tgtttgatgt
     4081 agatgtcttg ctatcacacc taaatgcgag ggcagaccta aggtagcgac caccttgcgg
     4141 ttgccataca cctcatccaa cagctgctta ggactaacaa tcccttgata aacgcctgcc
     4201 cgtgcaatag tgctataaat cagctcattc gagtacggaa caggaaagtt tctcatggct
     4261 aacctgcttg tttaaataag ctcgccatat ccactattac ccctttccct tttaaccgtt
     4321 catgcatggt tttttcaggt tggcgttgtg aatagatata acgtaaatcc gtatcaggca
     4381 agctatccca gtcgctgggc ttgactacct ttatagccga aaccttttta ctcttggagg
     4441 gcttttctag ttctgacact accgtttcgc cttccatcaa ccactgcaaa acaagaggca
     4501 gtaacttttg tcttgtcatc gttggattct ggctaaacgc ttttttaata gtgggaatta
     4561 acaggcttga atcgtaatcc tctttcagca tcagatataa atgacgctga tcttcggtat
     4621 ctaactcttg aagggctttt tcttctggtg tttgttcttg tatcgctgcg atatctagct
     4681 gaagttggat taaccgttta tcaatctcgg gaacgactag atcagaataa cgagcaatgc
     4741 gttctgggat acccgagcgt aatgcctcta gcatggggtg cacaggcttt aactcatctt
     4801 gatacacttg ccgcaataaa ccagcggtaa tacgctcatt gcctaaagct agcgcacgga
     4861 gctgagcgag tacaaaaagt tttactacaa tgtccatcac tccttggctt agctcatacc
     4921 acacatcacg gacctcatcc gataacagcg catctttgcg ttgtaaaagc tgtaattgcc
     4981 aaagattatc cgtaaaagcg atccactctt gattgggctt tccacgttgc gtttgttgta
     5041 taggatccca gaatatagct ccaaaccctg cccctctacg tgcagaccgc aaatcagcct
     5101 caaaaatctc tcgtgcttta ggggtaccaa tcaacatcac tggtacgcca ataatattca
     5161 ccatcgtcac aaaaaagttc agcatctctt gagatccacc cgaacgagag cggcttaaat
     5221 gctgaatttc atcaataacc aacaacccta aagcatgtgc attggctatt tgcgacatca
     5281 aagccaacat ggtttctata ccatgacgtt ttaagccata acgacgctca tagttcgagc
     5341 ccaaggctcg atccaacgct ctgaaaaaat tcaagcagat ttcttttagc gaaccattat
     5401 gcgagcagtc tattttcaaa tacaccacct gctctacatt gagttcacga tggtaaatca
     5461 cctgaggata cgtggctaga atacgatgaa gagaggtcgt cttcccacta ccagaacaac
     5521 caattaacaa taagctttgt gccgtagatc gtgcctcctc aaagcgaaat gtctccaact
     5581 ctcccgtttg aacacgctca taaccatttt gtaaatgctt ttgtaaatct cctgttttag
     5641 gatttctgcc tacgtagcca cctcgaatca tgaccgaaat acgctcactt agtagcaaat
     5701 gcgtacctaa tggctgaaaa tagtcatctg gaatacgaca aatggtatga gctctgataa
     5761 cacgggactt ttgcaagtca gaggaagtaa gctgtaaaga ggatttcagt gatgcagcac
     5821 tattcactga ctcttgtaat ggtggtaagg cctcgataaa aggattatca cgataagcct
     5881 ctacccccgt atcacgataa actgcttgaa tccgggtagc actcatgact catccttttc
     5941 tggtggatcc tgaaataatt caggcacgta tgtgggtagg ctgtaatctt cttgatcatc
     6001 cgcttccact gcgttgaaag gaataacttt agcctcatca cctgaagagc ttggcttcaa
     6061 atgctccgca cgttttttac gctccgaggt cacggcttct tttttattag ttttaatctg
     6121 cttaatgcgt gttgatttgg gctcagtagt actgggcgtt aacttattcg ctttctgaat
     6181 ggtttgctga ataaacgcct caagctccct gcgtttagtt aactcatcct gcttcgcatt
     6241 ggctttattg tgtttttctt gtgcttgtat atcccaaacc tcccaaaatg agagaccttt
     6301 aaactgccga ctacgttccg tcagattaca gcgccaaaat acacggctgc caacttgcgg
     6361 aaacaaataa atcgtatcaa ccagcactgg gtcataagcc gcttctaaat gttgaggtct
     6421 agctatatca gtgctccgct gcaaccaacc ctcacgcaga atctctgacc ccgagtaata
     6481 caaaccccac aaattaacgc caaatgaaga aatagagacc tttcggcgag gcagtaacgc
     6541 tactcgcaac tgctcttgct ccacagccct taaactacct gtacgatgct gcatacccca
     6601 ttgccatagc tggacaggaa tagacggtaa atctgtagga aaatcagcat ctcgatcgta
     6661 tttatccatc accagatggt tatttctgaa taagatcgta cgcaaaataa tttgtgtgaa
     6721 ctcaaatacc gacagagatg catctaacct atagtctgtt tcaccatggc ttttgatccg
     6781 actgccctct acaatgccag gtgcaaagga cttaaactcg gcttgtagtg ttctaaaagt
     6841 gctttccact atgcctttag catcgccacg tctaggtgga gcactttcca ctcgcacatt
     6901 aaaactagaa actaaggctt cgacctgatg actcattaat tcgccacggt ccgctagcaa
     6961 cacatctggc aaacctacac acggccagtc tgagctacta atctcaatat catgctgggc
     7021 acaaatggcc gttttgtcag agcaagcatt tacaaaagcc tgcatcgcca ccacataaga
     7081 cggattttca aagccgatat aaaagcccgt gatcatccga ctaaacacat caatcacaat
     7141 gtaaagcgtt ggtcttccta tgattttttg gcgatcatga tgatccacta aataaatatc
     7201 agcaatcgtg gcatcaatct cataacgact cccagggcct aacgcctgag aagtggctgt
     7261 actacttaag ggtcgtacgt cttttttata tacccctgct ttaactctag actttaagcg
     7321 ctgagcttta gggtattctc gatcataaaa ataacgaaac tgacgtagtg ttgggtaatc
     7381 ctcttgggga atgcgaggaa aatactgagc aaacaagtcc acaaatcgtc tataggcaac
     7441 ggtggtcttt gtaccttttt gatttaacag gtgcttttct atggtcaacc taaaaaggcg
     7501 ttcaatctcg ggcgttacct tggttccttc acccttacca tattctctgg ctcggccaat
     7561 ctttgctgtt cctgtcgctg aacgtctttc ccctggtgca ccgctgtttt tgtagtcagg
     7621 aattaatgca ttaggcgttt gaccacgctg ccagtaacgg cgtaacaact tataaactgt
     7681 agccttagta accttatgtt cttggaccac atgctcaacg agttcgcttc tgactttagg
     7741 gtcgaaacga tccttactat taataatagg aagaattttt cgataatcct cctcgcgctt
     7801 ctggaagctg acagaatcta cagacggctc ttctaaatca agatgtacat aagggtcatc
     7861 agttctaact aagcggcctt catctaaata ctgcattagc aacaactcag ccctagcttg
     7921 tggaactcct ttatcagcgc ttatttgcat ccagacaact tggccatcct ctatagccaa
     7981 aatgcgatac ggatcattat caaatagcac aacctcatta atttgccaca tagcgcaact
     8041 cctccatatt cactacttgg ctaatacaga gatctgcaca cttatttgcc ctgaaagact
     8101 tataaatatt gaacttaata aaaccatttg cggttaaggc tcgtatctca ctcaatgttt
     8161 tgcctaactc caaatcataa gcaatatcaa cctgcttaca gacattgata atgttttcat
     8221 ctcctttttc ttgcaggata tgggccaatg gggatagttg tgctaaaagc tccgcagaaa
     8281 cttcttctgt tttcactgaa taaagccatt caatattttc ttttactacg ggatttattt
     8341 ctttatcagt aaaaatgaac caaggaattt gcttttgctg ccaatagcga cgctctagtt
     8401 ctagtttttc taaggtacgc tcgtcttgta aggctgctgc aggtttgact tgaatagcaa
     8461 actgctcaaa aggaccatct ttgcagtcca ctaaaaaatc agtagacata acctgatcta
     8521 caccacgaat aacaggatgc ttaataccac tatctattgc aatctgcctg gtatcactag
     8581 gtaataaggg gaactgctcg cgtatatcta gcacgctgct ctcccactca agactgagaa
     8641 aaacagcaag ctctaagtca gatagcaaat gatggactcg tcccgtctta tgagaataaa
     8701 tacggtggga acgacctgaa gaaggaactt cttgtactgt tagccatgga atatagtctt
     8761 taccatgccc ttggccacgc ccctctttaa tacgacgggc aatttgcact tcagaaaatg
     8821 aagagtttgc tttagccata acaaaagtcc agtatgcttt ttcacagcat aactggactg
     8881 atttcagttt acaactattc tgtctagttt aagacttggg taccgagctc gaattcgcta
     8941 gcccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg tcaggtagga
     9001 tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc gtgcaaataa
     9061 tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt tgtcatggct
     9121 ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttt ggttagcgag
     9181 aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca attggtttct
     9241 tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc aaaatgatcc cctgctgccg
     9301 ggatactcgt ttaatgccca tctggtggcg ggtttaacgc cgattgaggc caacggttat
     9361 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt
     9421 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tttgccgacc gggtgatatt
     9481 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
     9541 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg
     9601 tcaatatttg ccaatacggg gttctttcgc ccggatgaag cgcaccagcc gcatttcagc
     9661 gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc ggagctgctg
     9721 gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc
     9781 catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca
     9841 gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc gtcgcgtctg
     9901 tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga ggaccaacgt
     9961 atcagccagg cgaagctgct tttgagcacc acccggatgc ctatcgccac cgtcggtcgc
    10021 aatgttggtt ttgacgatca actctatttc tcgcgggtat ttaaaaaatg caccggggcc
    10081 agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg
    10141 tcataattgg taacgaatca gacaattgac ggcttgacgg agtagcatag ggtttgcaga
    10201 atccctgctt cgtccatttg acaggcacat tatgcatcga tgataagctg tcaaacatga
    10261 gcagatcctc tacgccggac gcatcgtggc ctgtgggcgg acaataaagt cttaaactga
    10321 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
    10381 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
    10441 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggtcgacgcg
    10501 gccgtggcgc gcctcctagg tgcggatgaa tgtcagctac tgggctatct ggacaaggga
    10561 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
    10621 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
    10681 ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg
    10741 caggggatca agatctgatc aagagacagg atgaggatcg tttcgcggta caaccagttt
    10801 gcaagatgat taaacaataa cttaagttga agttacccca ttaaatggat ggctaatatg
    10861 actaaaaaaa tttcattcat tattaacggc caggttgaaa tttttcccga aagtgatgat
    10921 ttagtgcaat ccattaattt tggtgataat agtgtttacc tgccaatatt gaataattct
    10981 catgtaaaaa acattattga ttataatgaa aataataaat tacggttgca taatattgtc
    11041 aattttctct atacggtagg gcaaagatgg aaaaatgaag aatattcaag gcgcaggaca
    11101 tacattcgtg atttaaaaaa atatatggga tattcagaag caatggccaa gttagaggcc
    11161 aactggatat ctatgatttt atgttctaaa ggtggccttt atgatgttgt agaaaatgaa
    11221 cttggttctc gccatatcat ggatgaatgg ctacctcagg atgaaagtta tattaaggct
    11281 tttccgaaag gtaagtctat acatctgttg gcaggtaatg ttccattatc tgggatcatg
    11341 tctatattac gcgcaatttt aaccaagaat cagtgtatta taaaaacatc gtcaaccgat
    11401 ccctttaccg ctaatgcatt agcgttaagc tttatcgatg tagaccctaa tcatccgata
    11461 acgcgctctt tgtctgttgt atattggcca caccaaggtg atacatcact cgcaaaagaa
    11521 attatgcaac atatggatgt tattgtcgct tggggagggg aagatgcgat taattgggct
    11581 gtagaacatg caccacccta tgctgacgtg attaaatttg gctctaaaaa gagtttttgc
    11641 attattgata atccagttga tttaacgtca gcagctaccg gtgcggctca tgatatttgt
    11701 ttttacgatc agcgcgcttg tttttctgcc caaaacatat attacatggg aaatcagtat
    11761 gaggaattta agttagcgtt gatagaaaaa cttaatctat atgcgcatat attaccaaac
    11821 gccaaaaaag attttgatga aaaggcggcc tattctttag tccaaaaaga gagcttattt
    11881 gctggattaa aagtagaggt ggatgttcat caacgttgga tgattattga gtcaaatgcg
    11941 ggtgtggaat ttaatcaacc acttggcaga tgtgtgtatc ttcatcacgt cgataatatt
    12001 gagcaagtat tgccttatgt tcaaaaaaat aagacacaaa ccatatctat ttttccttgg
    12061 gaatccgcat ttaagtatcg agatgcgttg gcattaagag gtgcggaaag gattgtagaa
    12121 gcaggaatga ataatatatt tcgagttggt ggatctcatg acggaatgag gccgttacaa
    12181 cgattagtga catatatttc tcatgagagg ccatctcatt atactgctaa ggatgttgcg
    12241 gttgaaatag aacagactcg attcctggaa gaagataagt tccttgtatt tgtcccgtaa
    12301 taggtaaaaa gtatggaaaa taaatccaaa tataaaacca tcgaccatgt tctttgtgtt
    12361 gaaggaaata aaaaaattca tgtttgggaa acgctgccag aagaaaacag cccaaagaga
    12421 aagaatacca ttattattgc gtcgggtttt gcccgaagga tggatcattt tgctggttta
    12481 gcggaatatt tatcgcggaa tgggtttcat gtgattcgct atgattcact tcaccacgtt
    12541 gggttgagtt cagggacaat tgatgaattt acaatgtcta taggaaaaca gagcctatta
    12601 gccgtggttg attggttaaa tacacgaaaa ataaataacc gtggtatttt ggcttcaagc
    12661 ttatctgcac ggatagttta tgcaagtcta tctgaaatta atgtttcatt tttaatcacc
    12721 gcagtcggtg ttgttaactt aagatatacg cttgaaagag ctttaggatt tgattatctc
    12781 agtttaccca ttaatgaatt gccgaataat ttggattttg aaggccataa attgggtgct
    12841 gaagtctttg cgagagattg ccttgatttt ggctgggaag atttaacttc tacaatcaat
    12901 agcatgatgt atcttgatat accgtttatt gcttttactg caaataacga caattgggta
    12961 aagcaagatg aagttatcac attgttatca aatattcgta gtaatcgatg caagatatat
    13021 tctttgctag ggagttcgca tgacttgggt gaaaacttag tggtcctgcg caatttttat
    13081 caatcggtta cgaaggctgc tatcgcgatg gataatgatc gtctggatat tgatgttgat
    13141 attattgaac catcattcga acatctaact attgcgacag tcaatgaacg tcgaatgaaa
    13201 attgagattg aaaatcaagc gatttcgctg tcttaaaacc tattgggata gatattaccc
    13261 tatagatttc aagatggatc gcgacggcaa gggagcgaat cccgggagca tagcaaacta
    13321 tgtgaccggg gtgagtgagt gcagccaaca aagaagcaac ttgaaagata acgggtatag
    13381 ttaattctat cactcaaata taagggctct ctatgaaatt tggaaacttt ttgcttacat
    13441 accaaccccc ccaattttct caaacagaag taatgaaacg tttggttaaa ttaggtcgta
    13501 tttctgagga gtgtggtttt gatactgtat ggttactgga gcatcatttc acggagtttg
    13561 gtttgcttgg taacccttat gtcgctgctg catatttact tggtgcaacc aaaaaattga
    13621 atgtagggac tgcggctatt gttcttccca ccgctcatcc agtgcgccaa cttgaagatg
    13681 tgaatttatt ggatcaaatg tcaaaaggac gatttcggtt tggtatttgt cgggggcttt
    13741 acaataaaga ctttcgcgta tttggcacgg atatgaataa cagtcgcgct ttaacggagt
    13801 gctggtacgg gttgataaaa aatggcatga cagagggata tatggaagct gataatgaac
    13861 atatcaagtt ccataaggta aaagtaaacc cgacagcata tagtaaaggt ggagcccctg
    13921 tttatgtggt tgctgaatca gcctcgacaa ctgaatgggc cgctcaattt ggtttaccga
    13981 tgatattaag ttggattata aatactaacg aaaagaaagc acagcttgag ctttataacg
    14041 aggtggctca agaatatggg cacgatattc ataatatcga ccattgctta tcatatataa
    14101 catctgtaaa ttatgactca aataaagcgc aagagatttg tcgggatttt ctagggcatt
    14161 ggtatgattc ttatgtgaat gccacgacca tttttgatga ttcagacaaa acaagaggtt
    14221 atgatttcaa taaagggcag tggcgtgact ttgtattaaa gggacataga gatactaatc
    14281 gccgcattga ttacagttac gaaatcaatc ccgtgggaac cccgcaggaa tgcattgaca
    14341 taattcaaaa agacattgat gccacgggaa tatcaaatat ctgttgtggg tttgaagcga
    14401 atggaacagt agacgaaatt attgcttcca tgaagctctt ccagtctgat gtcatgccgt
    14461 ttcttaaaga aaaacaacgt tcgctattat agtagctaag gaaaaagaaa tgaaatttgg
    14521 attgttcttc cttaacttca tcaattcaac aactgttcaa gaacaaagta tagttcgcat
    14581 gcaggaaata acagagtatg ttgataagtt gaattttgaa cagattttgg tgtatgaaaa
    14641 tcatttttca ggtaatggtg ttgtcggtgc tcctctgact gtttctggtt ttttgctcgg
    14701 tttaacagaa aaaattaaaa ttggctcatt gaatcacatc attacaactc atcatcctgt
    14761 ccgaatagcg gaggaggctt gcttattgga tcaattaagc gaagggagat ttattttagg
    14821 gtttagtgat tgtgaaaaaa aagatgaaat gcgtcttttt aatcgccctg ttgaatatca
    14881 acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg
    14941 taatcccgat aatgattttt atagtttccc taaaatatcg gtaaaccccc acgcttatac
    15001 ccaaggcggg cctcggagat atgtcacagc aaccagtcat catattgttg agtgggcggc
    15061 taaaaaaggc attcctctca tctttaagtg ggatgactcc aatgatgtta gatatgaata
    15121 tgctgaaagg tataaagccg ttgctgataa atatggtatt gacttatcag cgatagatca
    15181 tcagttaatg gtattggtta actataacga agatagtcac aaagctaaac aagagacgcg
    15241 tgcatttatc cgtgattatg ttcttgaaat gtatcctaat gaaaatctcg aaaataaact
    15301 tgaagagata atcacagaaa acgctgtcgg agattatacg gaatgtatag ctgcggctaa
    15361 gctggcaatt gaaaagtgcg gtgcaaaaag tgtattgtta tcttttgaac caatgaatga
    15421 cttgatgcac caaaaaaacg taatcaatat tgttaatgat aatattaaaa agtaccacat
    15481 gtagtaaaag aatatggcag caacgctgcc atattctcta aattatttgg aggggtaaaa
    15541 caggtatgac ttcatatgtt gataaacaag agatcatagc aagctcagaa attgatgatt
    15601 tgattttttc cagcgatcca ttagcttggt cttacgatga acaggaaaaa atcagaaaca
    15661 aatttgttct tgatgcattt cgtaatcact ataaacattg tcaagaatac cgtcactact
    15721 gtcaggtaca caaagtagac gacaatatta cggaaattga tgacatacct gtattcccaa
    15781 catcagtttt taagtttact cgcttattaa cttctcagga gaacgagatt gaaagttggt
    15841 ttaccagcag cggcacgagt ggtttaaaaa gtcaggtggc gcgtaacaga ctaagtattg
    15901 agagactctt aggctctgtg agttatggca tgaaatatgt tggtagttgg tttgatcatc
    15961 aaatagagtt ggtcaactta gggccagata gatttaatgc tcataacatt tggtttaaat
    16021 atgttatgag cttggtagaa ttattatatc ccacgacatt taccgtaatg gaagaacgaa
    16081 tagattttgt taagacattg aatagccttg agcgaataaa aaatcaagga aaagatattt
    16141 gtcttatcgg ctcaccatac tttatttatt tgctctgcca gtatatgaaa gataaaaata
    16201 tctcatttta tggggataaa aacctttata tcataacggg gggcggctgg aaaagttatg
    16261 aaaaagagtc cctaaaacgc gatgatttca atcatctttt attcgacacg ttcaacctca
    16321 ataatattag tcaaatccgc gatatattta atcaagttga actcaacact tgtttctttg
    16381 aggatgaaat gcaacgtaaa cgtgttccgc cgtgggtata tgcgcgagca cttgatcctg
    16441 aaacattgaa acctgtacct gatggaatgc cgggtttgat gagttatatg gatgcgtcat
    16501 caacgagtta tccggcattt attgttaccg atgatgtcgg gataatgagc agagaatatg
    16561 gtcaatatcc tggtgtactt gttgagattt tacgtcgcgc caatacgagg gcacagaaag
    16621 ggtgtgcttt aagcttaaac caagcattta atagttgata tactctatgg attttaagat
    16681 gcatcgcgat ggcaagggag tgaattcgga tccgagctcg gtaccaagct tgatgcatag
    16741 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
    16801 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    16861 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    16921 cgctttccag tcgggaaacc tgtcgtgcca ggactacggt cgtgccaact tgcgtgataa
    16981 ctatcgtctg gttgatggcg catcgaaaga cggctggtta ttcactgctg aacatactca
    17041 gagtgtcctg aagggcttta acaagtttgt tgttcagtac gctactgact cgatgacctc
    17101 gcagggtaaa gggctgtcgc agggttctgg cgttgcattt gataacgaaa aatttgccta
    17161 caatatcaac aacaacggtc acatgctgcg tatcctcgac cacggtgcga tctccatggg
    17221 cgacaactgg gacatgatgt acgtgggtat gtaccaggat atcaactggg ataacgacaa
    17281 cggcaccaag tggtggaccg tcggtattcg cccgatgtac aagtggacgc caatcatgag
    17341 caccgtgatg gaaatcggct acgacaacgt cgaatcccag cgcaccggcg acaagaacaa
    17401 tcagtacaaa attaccctcg cacaacaatg gcaggctggc gacagcatct ggtcacgccc
    17461 ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag aaatggggtt acgactacac
    17521 cggtaacgct gataacaacg cgaacttcgg caaagccgtt cctgctgatt tcaacggcgg
    17581 cagcttcggt cgtggcgaca gcgacgagtg gaccttcggt gcccagatgg aaatctggtg
    17641 gtaagcttgc ggccgctatt gagggatctg attaattaaa acgtggctta ctaggatccg
    17701 atatcattta aatctaggga taacagggta atactagtgt cgaccaacca gataagtgaa
    17761 atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc
    17821 agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc
    17881 tcccagttcc caactatttt gtccgcccac aggctgaaag cgctatttct tcaaaacccc
    17941 cgtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat ccactagttc
    18001 tagagcggcc gccaccgcgg tggagctcca attcgcccta tagtgagtcg tattacgcgc
    18061 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
    18121 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
    18181 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gaaattgtaa gcgttaatat
    18241 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccgt
    18301 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
    18361 cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag
    18421 caaattcgac ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg
    18481 ctggtttacc ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg
    18541 aggccagttt gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc
    18601 tgcctcccag agcctgataa aaacggtgaa tccgttagcg aggtgccgcc ggcttccatt
    18661 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
    18721 agggcggcga ggcggctaca gccgatagtc tggaacagcg cacttacggg ttgctgcgca
    18781 acccaagtgc taccggcgcg gcagcgtgac ccgtgtcggc ggctccaacg gctcgccatc
    18841 gtccagaaaa cacggctcat cgggcatcgg caggcgctgc tgcccgcgcc gttcccattc
    18901 ctccgtttcg gtcaaggctg gcaggtctgg ttccatgccc ggaatgccgg gctggctggg
    18961 cggctcctcg ccggggccgg tcggtagttg ctgctcgccc ggatacaggg tcgggatgcg
    19021 gcgcaggtcg ccatgcccca acagcgattc gtcctggtcg tcgtgatcaa ccaccacggc
    19081 ggcactgaac accgacaggc gcaactggtc gcggggctgg ccccacgcca cgcggtcatt
    19141 gaccacgtag gccgacacgg tgccggggcc gttgagcttc acgacggaga tccagcgctc
    19201 ggccaccaag tccttgactg cgtattggac cgtccgcaaa gaacgtccga tgagcttgga
    19261 aagtgtcttc tggctgacca ccacggcgtt ctggtggccc atctgcgcca cgaggtgatg
    19321 cagcagcatt gccgccgtgg gtttcctcgc aataagcccg gcccacgcct catgcgcttt
    19381 gcgttccgtt tgcacccagt gaccgggctt gttcttggct tgaatgccga tttctctgga
    19441 ctgcgtggcc atgcttatct ccatgcggta ggggtgccgc acggttgcgg caccatgcgc
    19501 aatcagctgc aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc
    19561 aattacagat tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg
    19621 ttgcgtaccc ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc
    19681 tagttctttg gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg
    19741 ctccccctcc ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg
    19801 ccttgcccaa ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg
    19861 gggcaggcgg gcgggcttcg cccttcgact gcccccactc gcataggctt gggtcgttcc
    19921 aggcgcgtca aggccaagcc gctgcgcggt cgctgcgcga gccttgaccc gccttccact
    19981 tggtgtccaa ccggcaagcg aagcgcgcag gccgcaggcc ggaggctttt ccccagagaa
    20041 aattaaaaaa attgatgggg caaggccgca ggccgcgcag ttggagccgg tgggtatgtg
    20101 gtcgaaggct gggtagccgg tgggcaatcc ctgtggtcaa gctcgtgggc aggcgcagcc
    20161 tgtccatcag cttgtccagc agggttgtcc acgggccgag cgaagcgagc cagccggtgg
    20221 ccgctcgcgg ccatcgtcca catatccacg ggctggcaag ggagcgcagc gaccgcgcag
    20281 ggcgaagccc ggagagcaag cccgtagggc gccgcagccg ccgtaggcgg tcacgacttt
    20341 gcgaagcaaa gtctagtgag tatactcaag cattgagtgg cccgccggag gcaccgcctt
    20401 gcgctgcccc cgtcgagccg gttggacacc aaaagggagg ggcaggcatg gcggcatacg
    20461 cgatcatgcg atgcaagaag ctggcgaaaa tgggcaacgt ggcggccagt ctcaagcacg
    20521 cctaccgcga gcgcgagacg cccaacgctg acgccagcag gacgccagag aacgagcact
    20581 gggcggccag cagcaccgat gaagcgatgg gccgactgcg cgagttgctg ccagagaagc
    20641 ggcgcaagga cgctgtgttg gcggtcgagt acgtcatgac ggccagcccg gaatggtgga
    20701 agtcggccag ccaagaacag caggcggcgt tcttcgagaa ggcgcacaag tggctggcgg
    20761 acaagtacgg ggcggatcgc atcgtgacgg ccagcatcca ccgtgacgaa accagcccgc
    20821 acatgaccgc gttcgtggtg ccgctgacgc aggacggcag gctgtcggcc aaggagttca
    20881 tcggcaacaa agcgcagatg acccgcgacc agaccacgtt tgcggccgct gtggccgatc
    20941 tagggctgca acggggcatc gagggcagca aggcacgtca cacgcgcatt caggcgttct
    21001 acgaggccct ggagcggcca ccagtgggcc acgtcaccat cagcccgcaa gcggtcgagc
    21061 cacgcgccta tgcaccgcag ggattggccg aaaagctggg aatctcaaag cgcgttgaga
    21121 cgccggaagc cgtggccgac cggctgacaa aagcggttcg gcaggggtat gagcctgccc
    21181 tacaggccgc cgcaggagcg cgtgagatgc gcaagaaggc cgatcaagcc caagagacgg
    21241 cccgag
//
