LOCUS       sequence-330454-        4026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express MERS WT spike protein with an 12aa deletion on the 
            C-terminal tail. Used for MERS pseudotyped virus production..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Rogers TF, Zhao F, Huang D, Beutler N, Burns A, He WT, Limbo O, 
            Smith C, Song G, Woehl J, Yang L, Abbott RK, Callaghan S, Garcia E, 
            Hurtado J, Parren M, Peng L, Ramirez S, Ricketts J, Ricciardi MJ, 
            Rawlings SA, Wu NC, Yuan M, Smith DM, Nemazee D, Teijaro JR, Voss 
            JE, Wilson IA, Andrabi R, Briney B, Landais E, Sok D, Jardine JG, 
            Burton DR
  TITLE     Isolation of potent SARS-CoV-2 neutralizing antibodies and 
            protection from disease in a small animal model.
  JOURNAL   Science. 2020 Aug 21;369(6506):956-963. doi: 
            10.1126/science.abc7520. Epub 2020 Jun 15.
  PUBMED    32540903
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc7520"; journalName: "Science"; date: 
            "2020-08-21- 21"; volume: "369"; issue: "6506"; pages: "956-963"
COMMENT     Sequence Label: pCDNA3.3_MERS_D12
FEATURES             Location/Qualifiers
     source          1..4026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gccaccatga tccactccgt gtttctgctg atgttcctgc tgacccctac cgagagctat
       61 gtggacgtgg gccccgattc tgtgaagtcc gcctgcatcg aagtggacat ccagcagacc
      121 tttttcgaca agacctggcc tcggcctatc gacgtgtcca aggccgacgg catcatctac
      181 cctcaaggcc ggacctacag caacatcacc attacctacc aggggctgtt cccctaccaa
      241 ggcgatcacg gcgatatgta cgtgtactct gccggacacg ccaccggcac cacacctcag
      301 aaactgttcg tggccaacta cagccaggac gtgaagcagt tcgccaacgg ctttgttgtg
      361 cggattggcg ccgctgccaa tagcacaggc acagtgatca tcagccctag caccagcgcc
      421 accatccgga agatctaccc tgcctttatg ctgggcagca gcgtgggcaa tttcagcgac
      481 ggcaagatgg gcagattctt caatcacacc ctggtgctgc tgcctgatgg ctgtggaaca
      541 ctgctgagag ccttctactg catcctggaa cctcggagcg gcaatcactg tcctgccggc
      601 aatagctaca ccagcttcgc cacctatcac acccctgcca ccgattgctc cgacggcaac
      661 tacaacagaa acgccagcct gaacagcttc aaagagtact tcaacctgcg gaactgcacc
      721 ttcatgtaca cctacaatat caccgaggac gagatcctcg agtggttcgg catcacacag
      781 acagctcagg gcgtgcacct gttcagcagc agatacgtgg acctgtacgg cggcaacatg
      841 ttccagtttg ccacactgcc cgtgtacgac accatcaagt actacagcat catccctcac
      901 agcatccggt ccatccagag cgacagaaaa gcctgggccg ccttctacgt gtacaagctc
      961 cagcctctga ccttcctgct ggactttagc gtggacggct acatcagacg ggccatcgac
     1021 tgcggcttca acgatctgtc tcagctgcac tgctcctacg agagcttcga cgtggaaagc
     1081 ggcgtgtaca gcgtgtccag ctttgaggcc aagcctagcg gctctgtggt ggaacaagct
     1141 gaaggcgtgg aatgcgactt cagccctctg ctgtctggca cacctcctca ggtgtacaac
     1201 ttcaagcggc tggtgttcac caactgcaat tacaacctga ccaagctgct gagcctgttc
     1261 tccgtgaacg acttcacctg tagccagatc agccctgccg ccattgccag caactgttac
     1321 agcagcctga tcctggacta cttcagctac cctctgagca tgaagtccga tctgagcgtg
     1381 tcctccgccg gaccaatcag ccagttcaac tacaagcagt ccttcagcaa ccctacctgc
     1441 ctgattctgg ccaccgtgcc tcacaatctg accaccatca ccaagcctct gaagtacagc
     1501 tacatcaaca agtgcagcag actgctgagc gacgacagaa ccgaagtgcc ccagctggtc
     1561 aacgccaacc agtacagccc ttgcgtgtcc atcgtgccta gcaccgtttg ggaggacggc
     1621 gactactatc ggaagcagct gtctcctctg gaaggcggag gatggctggt ggcctctgga
     1681 tctacagtgg ccatgacaga gcagctccag atgggctttg gcatcaccgt gcagtacggc
     1741 accgacacca atagcgtgtg ccctaagctg gaattcgcca atgacaccaa gatcgccagc
     1801 cagctgggca actgcgtgga atactctctc tacggcgtta gcggcagagg cgtgttccag
     1861 aattgcaccg ccgtgggcgt ccgccagcag agatttgtgt acgacgccta ccagaacctc
     1921 gtgggctact acagcgacga cggcaattac tactgcctgc gggcctgtgt gtccgtgcct
     1981 gtgtctgtga tctacgacaa agagacaaag acccacgcca cactgttcgg cagcgtggcc
     2041 tgtgaacaca tcagctccac catgagccag tactccaggt ccaccagatc catgctgaag
     2101 cggcgggata gcacctacgg acctctgcaa acacctgtgg gctgtgtgct gggcctcgtg
     2161 aatagcagcc tgttcgtcga ggactgcaag ctgcctctgg gccagtcttt gtgtgccctg
     2221 ccagacacac cctccacact gacacctaga tctgtgcgct ctgtgcccgg cgaaatgaga
     2281 ctggcctcta tcgcctttaa tcaccccatc caggtggacc agctgaactc cagctacttc
     2341 aagctgagca tccccaccaa cttcagcttc ggcgtgaccc aagagtacat ccagaccaca
     2401 atccagaaag tgaccgtgga ttgcaagcag tacgtgtgca acggcttcca gaagtgcgaa
     2461 cagctgctca gagagtacgg ccagttctgc tccaagatca atcaggccct gcacggcgcc
     2521 aacctgagac aggatgatag cgtgcggaac ctgttcgcct ccgtgaagtc ctctcagagc
     2581 agccctatca tccctggctt cggcggcgac tttaatctga ccctgctgga acctgtgtcc
     2641 atcagcaccg gctccagaag cgccagatct gccattgagg acctgctgtt cgacaaagtg
     2701 accattgccg atcctggcta catgcagggc tacgacgatt gcatgcagca gggacctgcc
     2761 agcgccaggg atctgatttg tgcccagtat gtggccggct acaaggtgct gcctcctctg
     2821 atggacgtga acatggaagc cgcctacacc tctagcctgc tgggatctat tgctggcgtt
     2881 ggctggacag ccggcctgtc tagctttgcc gctattccct tcgctcagag catcttctac
     2941 cggctgaatg gcgtcgggat cacccagcag gttctgagcg agaaccagaa gctgatcgcc
     3001 aacaagttca accaggctct gggcgccatg cagaccggct ttaccacaac aaacgaggcc
     3061 tttcagaaag tgcaggacgc cgtgaacaac aacgcccagg cactgtctaa gctggcctcc
     3121 gagctgagca ataccttcgg agccatctct gccagcatcg gcgacatcat ccagagactg
     3181 gacgtgctgg aacaggacgc ccagatcgac agactgatca acggcagact gaccacactg
     3241 aacgccttcg tggcccagca gctcgtcaga agtgaaagcg ctgcactgtc tgctcagctg
     3301 gccaaggaca aagtcaacga gtgcgtgaag gcccagagca agagatccgg cttttgtggc
     3361 cagggcaccc acatcgtgtc cttcgtggtc aatgccccta acggcctgta ctttatgcac
     3421 gtgggctatt accccagcaa ccacatcgag gtggtgtccg cctacggact gtgtgacgcc
     3481 gccaatccta ccaactgtat cgcccctgtg aacggctact tcatcaagac caacaacacc
     3541 cggatcgtgg acgagtggtc ttacaccggc agcagctttt acgcccctga gcctatcacc
     3601 tctctgaaca ccaaatacgt ggcccctcaa gtgacatacc agaacatctc caccaacctg
     3661 cctccacctc tgctgggcaa ttccaccggc atcgatttcc aggacgagct ggacgagttc
     3721 ttcaagaacg tgtccaccag cattcccaac ttcggcagcc tgacacagat caacaccaca
     3781 ctgctggacc tgacctacga aatgctgtcc ctgcaacagg tggtcaaggc cctgaacgag
     3841 agctacatcg acctgaaaga gctggggaac tacacctact acaacaagtg gccctggtac
     3901 atctggctgg gctttatcgc tggactggtg gcactggccc tgtgcgtgtt cttcatcctg
     3961 tgctgtaccg gctgcggcac caattgcatg ggcaagctga aatgcaaccg gtgctgcgac
     4021 agataa
//
