LOCUS       sequence-33065-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP220
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        182..369
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttatcgt
       61 tttaagagct tggtgagcgc taggagtcac tgccaggtat cgtttgaaca cggcattagt
      121 cagggaagtc ataacacagt cctttcccgc aattttcttt ttctattact cttggcctcc
      181 tctagtacac tctatatttt tttatgcctc ggtaatgatt ttcatttttt tttttccacc
      241 tagcggatga ctcttttttt ttcttagcga ttggcattat cacataatga attatacatt
      301 atataaagta atgtgatttc ttcgaagaat atactaaaaa atgagcaggc aagataaacg
      361 aaggcaaaga tgacagagca gaaagcccta gtaaagcgta ttacaaatga aaccaagatt
      421 cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat cttcccagaa
      481 aaagaggcag aagcagtagc agaacaggcc acacaatcgc aagtgattaa cgtccacaca
      541 ggtatagggt ttctggacca tatgatacat gctctggcca agcattccgg ctggtcgcta
      601 atcgttgagt gcattggtga cttacacata gacgaccatc acaccactga agactgcggg
      661 attgctctcg gtcaagcttt taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt
      721 ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga tctttcgaac
      781 aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga tctctcttgc
      841 gagatgat
//
