LOCUS       sequence-33069-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP316
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        41..322
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
ORIGIN
        1 cccgggataa cttcgtatag catacattat acgaagttat aacgacatta ctatatatat
       61 aatataggaa gcatttaata gaacagcatc gtaatatatg tgtactttgc agttatgacg
      121 ccagatggca gtagtggaag atattcttta ttgaaaaata gcttgtcacc ttacgtacaa
      181 tcttgatccg gagcttttct ttttttgccg attaagaatt aattcggtcg aaaaaagaaa
      241 aggagagggc caagagggag ggcattggtg actattgagc acgtgagtat acgtgattaa
      301 gcacacaaag gcagcttgga gtatgtctgt tattaatttc acaggtagtt ctggtccatt
      361 ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag attccgatgc
      421 tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc cggttattgc
      481 aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc cgaaatactt
      541 ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca atgattacgg
      601 cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag agttcctcgg
      661 tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac tcagtgcagc
      721 ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg ggacaggtga
      781 acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc ccgaaag
//
