LOCUS       sequence-33073-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP412
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(24..57)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtcgactcta gaggatcccc gggataactt cgtatagcat acattatacg aagttatggg
       61 cccattatga atacgcacct ctatgtattt ccgagatacg attactccag ttcctcttac
      121 aagaaatgca taaaaatagt tacaattaat tagaacaaga acttatttag aacacgttca
      181 cactgagtaa gaactcttgt cccttattag ccttgatagt gctgaaaaaa agaaaaaaaa
      241 caaaaaaaag aaataaaata acggcaaaca gcaaaggcca cagaaccgta ttcatgttac
      301 ttctgcaata tcaatcactt actggcaagt gcgtataaat taaacctatt tctttatcat
      361 catatttact tatatcttta acagattcca aaccctaaag tgtccgaatt ttcaataggg
      421 cgaacttgaa gaataaccaa ggtcaataat atatctttta gtataaccct gaaatttgcc
      481 ctatagaaat ctagggtttc tgtgtggttt ccgggtgagt catacggctt ttttgaattt
      541 ctttttttgc agttgtctct atcaatgaaa atttcgagga agacgataag gttaagataa
      601 gtagataaga gaatgatacg agataaagca caaattagca gaaagaagag tggttgcgaa
      661 cagagtaaac cgaatcaggg aatccctttt tgcaaaaaca tcaattatcc ttttcttttt
      721 tttacgtata tatctggaac agaaatatat aagttactat tatacttata gttggatcca
      781 gtttttaatc tgtcgtcaat cgaaagttta tttcagagtt cttcagactt cttaactcct
      841 gtaaaaacaa aa
//
