LOCUS       sequence-33079-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP518
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(55..88)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(617..633)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(617..633)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(630..652)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    641..657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(665..671,672..689,690..695))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    710..731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(848..865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt acccataact
       61 tcgtatagca tacattatac gaagttatcc cgggtaccga gctcgaattc gtaacttaca
      121 cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt
      181 ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga
      241 agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata
      301 tttattagac aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg
      361 taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat
      421 taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga
      481 gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct
      541 atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg
      601 atgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      661 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      721 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      901 ctcactc
//
