LOCUS       sequence-33081-e         644 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Fang F, Salmon K, Shen MW, Aeling KA, Ito E, Irwin B, Tran UP, 
            Hatfield GW, Da Silva NA, Sandmeyer S
  TITLE     A vector set for systematic metabolic engineering in Saccharomyces 
            cerevisiae.
  JOURNAL   Yeast. 2011 Feb;28(2):123-36. doi: 10.1002/yea.1824. Epub 2010 Oct 
            8.
  PUBMED    20936606
REFERENCE   2  (bases 1 to 644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/yea.1824"; 
            journalName: "Yeast"; date: "2011-02"; volume: "28"; issue: "2"; 
            pages: "123-36"
COMMENT     Sequence Label: pXP522
FEATURES             Location/Qualifiers
     source          1..644
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    469..502
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    525..558
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc
       61 cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc
      121 ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga
      181 aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct
      241 tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt
      301 gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc
      361 caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta
      421 aaagatacga ggcgcgtgta agttacgaat tcgagctcgg tacccgggat aacttcgtat
      481 aatgtatgct atacgaagtt atggtaccgg cggccgcccc cgggataact tcgtataatg
      541 tatgctatac gaagttatgg attttcttaa cttcttcggc gacagcatca ccgacttcgg
      601 tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgca
//
