LOCUS       sequence-330862-       13527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of AvCAST proteins (except TnsD). Two BsaI 
            sites for spacer cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13527)
  AUTHORS   Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, 
            Macrae RK, Zhang F
  TITLE     Dual modes of CRISPR-associated transposon homing.
  JOURNAL   Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 
            10.1016/j.cell.2021.03.006. Epub 2021 Mar 25.
  PUBMED    33770501
REFERENCE   2  (bases 1 to 13527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.03.006"; journalName: "Cell"; date: 
            "2021-04-29- 29"; volume: "184"; issue: "9"; pages: "2441-2453.e18"
COMMENT     Sequence Label: pMS6: pHelper(AvCAST)_entry_Delta-TnsD
FEATURES             Location/Qualifiers
     source          1..13527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(28..46)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    307..331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(332..350)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        396..473
                     /gene="lacI"
                     /label=lacI promoter
     CDS             474..1556
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(493..512)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        2617..2708
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2709..3500
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     protein_bind    9312..9336
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9337..9355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(9504..9551)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     9547..9565
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ggggaattgt tatccgctca caattcccct atagtgagtc gtattaattt cgattatgcg
       61 gccgtgtaca atacgattac tttctgttcg acttaagcat tatgcggccg caagcttgtc
      121 gacctgcagg cgcgccgagc tcgaattcgg atctgctcaa cgcctaacga catctaatgt
      181 taaagcacgc caacaaacaa tgctttatga atcctcccag gagactgctc aacgcctaac
      241 gacatctaat gttaaagcac catggtatat ctccttatta aagttaaaca aaattatttc
      301 tacaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcctaat
      361 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct
      421 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
      481 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
      541 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
      601 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
      661 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
      721 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
      781 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
      841 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
      901 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
      961 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
     1021 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
     1081 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     1141 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     1201 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     1261 gcgttggtgc ggacatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
     1321 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     1381 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     1441 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     1501 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     1561 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg
     1621 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactaa catgagaatt
     1681 acaacttata tcgtatgggg ctgacttcag gtgctacatt tgaagagata aattgcactg
     1741 aaatctagag cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1801 gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt
     1861 aagcagttcc ccaactgact taaccttcga aaaaaccacc tggggaggtg gtttgatcga
     1921 aggttaagtc agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa
     1981 ccaaatctgt ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt
     2041 ttagctaaac aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt
     2101 accgtgcttt tccgggttgg actcaagtga acagttaccg gataaggcgc agcagtcggg
     2161 ctgaacgggg agttcttgct tacagcccag cttggagcga acgacctaca ccgagccgag
     2221 ataccagtgt gtgagctatg agaaagcgcc acacttcccg taagggagaa aggcggaaca
     2281 ggtatccggt aaacggcagg gtcggaacag gagagcgcaa gagggagcga cccgccggaa
     2341 acggtgggga tctttaagtc ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc
     2401 ctcacggctc ccacagatgc accggaaaag cgtctgttta tgtgaactct ggcaggaggg
     2461 cggagcctat ggaaaaacgc caccggcgcg gccctgctgt tttgcctcac atgttagtcc
     2521 cctgcttatc cacggaatct gtgggtaact ttgtatgtgt ccgcagcgcc cgccgcagtc
     2581 tcacgcccgg agcgtagcga ccgagtgagc tagctatttg tttatttttc taaatacatt
     2641 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     2701 ggaagagtat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2761 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2821 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2881 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2941 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     3001 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     3061 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     3121 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     3181 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     3241 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     3301 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     3361 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3421 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3481 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgaggggcc gcaagatccg
     3541 gccacgatgg tgtacaatac gattactttc tgttcgactt aagttatgac tgaaagaact
     3601 cttctataga atttgctaaa aattttatca ttaagatatg agttatgcgg tcaattgctg
     3661 ggtttaagtc gcatccaaac aagcagtgag aaaaatattt accttgattt tgggtcagct
     3721 ttggattgag ggcttctaag gattcttgac cataaaactc aaatataagc tgggcaaaac
     3781 cacaatcatt aaacttaaac ttgccaccag caaaggtttt tagcagtcct ttatccaata
     3841 agtaacgctg gtactgagcg cgaagccaag ccaaaccttg aaaggcaaaa ctgctatgaa
     3901 ttagccaatt aatatcatgt gccagtattg aaagtgtttc tacagtttta tttgtcaatt
     3961 ttttcacatt atttgctaat aaacaattat ctatgctggc agcatgataa tgtatgcctt
     4021 tcgattcaac ttctatacag ctattgtgta aaacaacacg atgaattgga caaactaaaa
     4081 tgccagggac ttgatgtatt ctatgccagt aaggttcacc atacttttgg gtatctttct
     4141 ctaaacattc agggcaaaat tttaaaaatt tttttgaatc atcactagaa ttattagcca
     4201 ctttagcaaa atctagtata gacccactga atttattact cattgagttt ttcagcagcc
     4261 aagcctcctg cggcattaat aaagttgcat aaaaggaata taaagtatga ttttgtagta
     4321 aattctcaac tttttgctgg gagcgtaaag gtaagttttt cactaaataa tttaaattat
     4381 tagttaaggt aattttgcat atttgttggg aactgtaacc gaatagctct aagtcagttt
     4441 gcttgaaact tttatttcca ctccgaatat ggtatctagc taaagtgcta tataaaagtt
     4501 catcgggata cagagttggg aaaaaactaa gcatttagaa gctaaatatt gataaaatct
     4561 tcaactggat ttttgattat acctgctaat ttcaaatcct cgtatgcaga ttttttgtct
     4621 ttcttggcat tctcgacaat tcgccgaata tcattctctt ggtactttgt ctttaattta
     4681 gctttcctgg tacttgtttt agcaagactc tcatttactt gacatccttg caaagcaaga
     4741 gcatatgcct tattaactaa agatggaatt ggaatgtctg gatcttcatg actataaaca
     4801 atttgttctg cacattcctt tgctttacca gattctactt ccaatttcaa cagctcacta
     4861 attacataat tgagcttcgg agatactttc tgctgattct gctgcttttt agctaatctc
     4921 ttaatctctg tcaaatcttt agcatcaatc tcagatacac atctattgtg atattccgta
     4981 atatcgagag gagcaatatc cttatattta atcatccact ctctgtcccc taacctaatt
     5041 gcatctagca ttggctttac taaatacaat ccttctttag ctacttgacg aatcagatca
     5101 accgtaataa tttcttggtc acctattgaa atcgctctcc attgaaccat tttgtaaagt
     5161 tttatagcaa tatcaataat tccctggctt tcatcataaa aaacttcttt tatttcatcc
     5221 gagaaatgaa caggtgtttt tgtccattgg tattcccata ttccttctaa aaagtaacac
     5281 cattcatcat tatttaccat tctatcccac attctacttc cttctccagt atttcttcta
     5341 gcatttctaa gattaccttg aagaaaacta atggcttcat tcgtacctac acgaataact
     5401 ggaattccaa tagtattatc gagctttact aaaaaattta atatttcgtc tctatttctt
     5461 tttgcagttg ctaaattttg tatttcatca atcaccaaaa ctcctagatg atgagtgtgt
     5521 gctgtttgtg caagttgagc caatagataa tcttcagatg tgcgtgctgg gttaaatttc
     5581 tttaataaat tagttcctaa caatttatcg atacctgcaa aagtatctat acataacccc
     5641 tttaaagttt tagggcaatc tacccgcagc caaactattt ggtaaacact ataatcagga
     5701 tgaaaaatta tttggggata aagctcaaga atatttttga aatttgtagt ctttccaata
     5761 cctgatgggc caattagagt taagccggac gcagatgtag gaagattaac atagttttct
     5821 aaatttttac ctcctccatt acctattgca ttataaattt gcctagagcg atttgcatat
     5881 tcaggtttta aaaggttcct tgacagatag cctgtcatga taagcgtaca aataactttt
     5941 tgtaattcaa gagttttatt ttgagggtca aaatatctag ataacctctc aatacaatga
     6001 aatctataat gtggttctag ctctctctct tgttcatcaa aatttggttc tctggctact
     6061 ttatctataa attctgctgc tgataggata ggaggtaatg cctgaattaa tggattagtg
     6121 ttgtattctg gcagtttttg atctatatat ctggcaataa ctgctagttc tccattagga
     6181 attttaacca gttcactctc aattctgtcc acgttttcgc tcctgtcgct ttttcttaag
     6241 aagattcaga tggtttggct gtagtgattc tggctgttcg gaggtttctg ctgtactctc
     6301 tgcaacctga aaactgctct gattatctgt aattgctaat tcaaatcctt cattctctcg
     6361 tcgtttagcc ttttcaaaag ccctattatc tcttattcca gaaactttct tgctcttact
     6421 taaattatga tattgagaag catcagtcat tttttctgct ctatctacta tgctttcaat
     6481 atcagctatt aaatctacct tttcttgtag ctgtttacct tggcttttct gtctttgcaa
     6541 ttcttcataa gcaagcagat attcaatatc atgaaagttt ttattagcat accgcccttc
     6601 taagtcaata agaaaacatt tttcaaaatc tctaccatca ctagatggaa gataaatgaa
     6661 gtttggcttt ctgatgtcat aagatacaat caatgatttt tctgacttag aaaggagatt
     6721 acttctagct ttttcaaacc attgctcatt tttagcacta tcacaggtat agtacatctc
     6781 cttacctctc attttgatgc ctttagcagt aatagttgct ttctcagtag gcatcaagtt
     6841 caatttgaca atatcttcag aaaaagttct gagtctgcca gaacgattgg cgatacccca
     6901 cttccacaac tctctaggta taggaggggt atcatcagca atcattgctt catctctttc
     6961 atattcagta agaggtttat tgttatggtg aacaattaaa cgaataataa tctcagtaaa
     7021 ttgatctata tcaagtttgc catcaagcct ataatccctt gcacctcttt gcctaaaatc
     7081 cgtatccaca taaccaggta aaaaaggttt tacatattca tgaattatac gaaagtgtct
     7141 ttctactaac cctttccaat ctgctctata ggaagctgca ttttcaatac gaacataaag
     7201 attagggatt aatgtttcta ctgtcatacc tgctaattct cccctatctc ctaaaattgc
     7261 atctggcatt tgattgcaag gccattcatt ttcagtaata ttaatgttat attcctggca
     7321 aaacagggtt ttatctgtag ctacattggc tagtgccatc attgctccta tccaagatgg
     7381 gccttctaaa ccgacataaa cacctgtaat cattctacta aaaacatcaa taattacata
     7441 gataactggt ctaccaatta tccagtcacg gttatatttt gaaactaaat atacatcagc
     7501 aactgtggca tcaatttgat atctagaacc agggccaata gtttccatct tagagtttcc
     7561 caaaattgct ctattctcta aagctactcg cttggctcct tttcgagatg ttaaagtttt
     7621 ggtaacatca tcctgttcaa gttgatacca gtatttgaat tgagtaattg taggtatttt
     7681 atcagttggg gttaatatag atttttgaac accattctcg tcatatctaa ttccttcctt
     7741 gtaatattct ttcaccatta attcataggc tgttgttaag tagttttctt ttggattatt
     7801 gtaaaatttg cttatagata ctctaaaaat ctttctatct tcttctgtaa catttacacc
     7861 ttcaccaata tctgggtcat gtgcatattt tcttggtctt cctctcttct tgcccgattg
     7921 tcctcgaatc ttgcccttac ctgatgaatt gataaaatca ggtatgagag catttatagt
     7981 ttttcctctc tgccaaaatc ttctcaaata accatagacg gttttttcta ctaatttatc
     8041 tttatccctt tcttcgttgt acttttcaac cgcttcttta accatcgatc ctctaaaatt
     8101 tctataatat attgatggtt cttctataat taatgatgaa attatctccc aagctttatc
     8161 tctgtattcc ttttccttat ctgtcaaatt ttcatcccga ataattcttg cccaaggatc
     8221 ttgttgctgc ttaaaagcaa taccttcagt tatagcttct aaaatatctg atattttcct
     8281 tggttcaggt agacctttct tagcattaat atctataaca aatgcaataa tgtaaccttc
     8341 atctatccaa ataattcttt cgactatatg agtgttttgg tatacaatcc actctattaa
     8401 atcattgaca aataaatctt taaccatatt atttacctat catgtgggtc taaaatatta
     8461 ttttttcaat aactttagtt gacggacaag cagataattt tattgctact agatccacaa
     8521 ggatttcttt cctagcaatt aaatgtttaa atacataaag tgatgtaccg gaatttatat
     8581 tcatttcttt gtctaaagca gtagttactc tatttatagt tgtatcttct tcctgaagct
     8641 tagattttaa aattgttgac aattcactca gttgtgcagt gtccatttca gaatttgctt
     8701 ctagcctata agcagaatga acccactcaa cattttcagc taaaagcctg ggaatttctt
     8761 tttctgtgat aattccccag tcaataccct ttgctaagta atattgtctt tctatctcga
     8821 atttctcatt aacttgcttt ttctccagtt ctgttgacgg cttaacagtc cgggcaatct
     8881 gcatctgctt cccatcgcgg ttgactgtaa gcataaagtc gcttgttaaa acgtgaggag
     8941 tgccactttg tgtatctaca gggtacttta tacctatgtc tgtagcaata ctcatggcta
     9001 gatcgagatt gtctaaagga aattgctctc ttatatctgt gactatatct gaccactcaa
     9061 acaaataaaa tagccgtttt tcatggtctg agaagaaatg atgtactcga tttgttttcc
     9121 aaccaggagt tctagaagaa cgtcctttag agggaacatc ttggattgta agccaaggtt
     9181 tatagtgctt accacttcct cggccacgac cttcttttat atatcgctca tatttagctt
     9241 gagtccattc ccttctaccc ctaaccatgg tatatctcct tattaaagtt aaacaaaatt
     9301 atttctacag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc
     9361 ctaatgcagg agtcgcataa gggagagcgt cgagatccag ggcagggtcg ttaaatagcc
     9421 gcttatgtct attgctggtt taccggttta ttgactaccg gaagcagtgt gaccgtgtgc
     9481 ttctcaaatg cctgaggttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg
     9541 ggttatgcta gttattgctc agcggtggca gcagcctagg ctaattactt tgaactgaag
     9601 tccaagataa ttcaggtggt tgaaagactg gacatttctg aatctcgtaa cgtagccatc
     9661 gcgttccccg cgaacctaca tggtctaccc agtaaggtaa agttagtaac ccatattcat
     9721 ctcgaattaa ccatcgcatt gattctgcat agtaattttt aggtaaaagg gtaacatcat
     9781 tcactaaatc ccgactttca cctaaacata atccaccaaa cctattaata gaagccgggt
     9841 tagtcaatgc ctctgctaaa cgttccacta aatttggttt agctttatct actccagcat
     9901 ccacccaaac tataaattct atattagtta ataactcctg ataatcaggt ttattatttt
     9961 tagagtcatg gatattcttc gtcttaaatc ggtgaaaggt gcgaataaca gttgactttt
    10021 ctggttcaga tagaagtgcg atcgccagct tcacaccgca gtgtttgtat ctatccactt
    10081 ctccaacaag agatagtaac attccatata ctgtcgatgg tggtggtaca gggtaagttt
    10141 cgccatattc cctagcacga gactgccgaa aacaagctat gggaacttct acttttaagg
    10201 caattgtcgt catatcatga actccttaac gacaactgca aatcttcaat aatcacctgt
    10261 tttaatgctt caactgttgt cttcactccg ggaaacacat ttgcccctaa atcttccaga
    10321 gtgctagaaa ttgcccctcc tatccataat tctttagggt ctatatctcc agattctacc
    10381 cgccttacta aatccggtgt tgatatattt ctaagttcat cttcttcaaa acagtaaagt
    10441 aaccgaggtg agaaatcgtg tgtccaccgt aatatgatgc tatcaggaga aaaatcatag
    10501 agaaaacgag cgtgatttcc tgccacttct ccaatagaaa ttaagctatc gaggacagca
    10561 ttaatgcgag atttatcttt gaggctattg ggtgttaatg cgaagccata ttgatatcga
    10621 gtagcatgaa cttctgtaga ataaagagat gttcgtcctt tctttccact catagcattg
    10681 aaagtaagat caccagcata aggaattgtt gaaactgcac gagtgacttc taatacacct
    10741 cgtctagtag ttattttccc tttaggtttt tccttacgct tacgttttgc agtttctttc
    10801 tccttacctt gagaaagttt tttgtcctca tcttgagttg agttctggat ttcaacttcg
    10861 gattcaactg ctgcttcaac ttttgctgct tccgcttgca taaatcccaa gacatcatca
    10921 tcaatgaagc gaatatcatc aaaattagga tcttgcaaga tgtgatcggg tactggttga
    10981 gcattttcat cccaacgtct attcacagga tagccagcat tttgccaata atatcgcaat
    11041 gcccaacgaa ttgcctcaga tgaaacagta gagtgaactt cacctttcca aattaatttt
    11101 tgtaacgtag tggtattacc ttcattttca ccacgattat tagcggcggt tccataacta
    11161 gtcaaaatat taccaaacag atgaaacatt ccaccattac ctaaacaatt aatattttgt
    11221 tcttaatcaa atcaaaaaat atgaagttta ttcagtattt agctccgata tttgtgatgg
    11281 ttttacctct acctctgtta ttttattctt tgggtagcta gccattgcaa taaaagttaa
    11341 gtccctggcc actttccaat tgcctatccc tgtagtcaat ggaagtaatt ccatccaatg
    11401 ttcttccaga attgctattt gccctgccct tccccaaaac tctgcaataa attttcggaa
    11461 attgtcagta ttggtgcagc gttttaactg agataaaatg cgtgtatttt ctcgttctat
    11521 ttgagcgtat tcaccttctt ttgttctgct ataaattttg gcatatattt ttcttaatgc
    11581 ttcatgacaa gcttgaataa atagcttttg agcttctata tcccattctg agtcctgaat
    11641 catcttatta attcctttat actcatcagt aaattgttta aatgattttt tatttttaat
    11701 aaatgaaatc caattagccc accaagaccg acccattgct agattattgg cgattattcc
    11761 ttttattaaa cttggaagca aataatgttg atttttatat aaaacaattt gacattctgg
    11821 aaaacaaata caactaagct tataaagata attaactgaa gaatttaatt ctatgattgc
    11881 tatttctgca cgagtttttt gctgttctga ccaagctttt gtactaaaaa agattatttg
    11941 acatcgtttt aaaaaattgg aagtaggcat ttgtgttgtt ttataattaa gaaattttaa
    12001 tccagcatct cctaagctag atacgtgaaa atgtttataa tctaagttac ccaaacacca
    12061 caaaaaatgt gcataaagtt ctaaatcaat tacttcagga ataaccaaaa catagtttgt
    12121 atgtgatggt tcggtgtgcg atcgcagcac aaaatattga caagccatag gagcgaacaa
    12181 caacgctaga gcatattctg ctttttcttc aaatgctgtt tgttctttaa aagcataatg
    12241 gcgaactact gctcctggat agagccatcc cctgatacca attggctctt ttagcaattg
    12301 tccgtactgg tcacataaat gtttagcaaa atgttgatga gcataggaag cagctttctt
    12361 ataatcaacc ctaacctgaa taccatcaat aattagttgc ttagattgtt gacccgcaga
    12421 cttaaaaaat ttattatgtt gtgtaaaagt ggctgtgata ccttgatgga taattatttg
    12481 attttgaata tccatgtttt gagaatctaa ccctgtcagg gaaattaaac cctcatcgct
    12541 gatttgaaat gattgcttca gcaaccagtc taaaactgtg aaatcctgtc cttcccaatc
    12601 aagattgata tagtgagggg ttagcaacca agttaaatta ccaatacgtt cagcaggtgt
    12661 tggatagatt ttttccagtt ttttgagcgt catccaaagt cctgcaaccc cagcacgatg
    12721 caataaagtc atactaggat cgccgagatt aagctgaatt ttggggttat ctgctctctt
    12781 catcatctgc tttcgagtaa ttgctattgc ctttccttaa taggcatgaa gagtccacaa
    12841 cccatctttt gctttcctcc tacaccatgt atttgcaacc tcagtgagtc ttcatcgctt
    12901 aaatttataa cttctaatcc aaaacccacg actgtaaatc gcttaatttt gatagtccta
    12961 cgtattggtt tgccattggc tttggtggga atgctagcaa ttgcttggat acccagttgt
    13021 tctagctgac gctgcgctac gactaaaaaa ctttcaggtt cttcatagcc tcttattacc
    13081 acaatgcgcg atcgcagttt ctccgcaggt tgaagcaaac agatgtcagg aatacccaaa
    13141 cgaattgtat gcttgccaat tctcagtgac ttacccgcca atggataaac caaacgtacc
    13201 tgatttactg gtatccgaat acgaagccta gaattttggg tcagattaat tacaccatcc
    13261 ttgggaatac ctgcaatggt ctgaattccc aacgtgtcta atgtatgtag tttgggttca
    13321 aagtgggcga tcgcagaaaa taattcatag ccgtggtcga aaggtaatgt ctcacccgta
    13381 aggctaaaag ttaaatccat atatggaaac tgagacatta aaagctctct tggctttgaa
    13441 gatgattctg agggaagctg cgtaactaag ttactatcaa taatcatatg tatatctcct
    13501 tcttatactt aactaatata ctaagat
//
