LOCUS       sequence-330870-        9595 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human CPSF6 fused to iRFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Zhong Z, Ning J, Boggs EA, Jang S, Wallace C, Telmer C, Bruchez MP, 
            Ahn J, Engelman AN, Zhang P, Watkins SC, Ambrose Z
  TITLE     Cytoplasmic CPSF6 Regulates HIV-1 Capsid Trafficking and Infection 
            in a Cyclophilin A-Dependent Manner.
  JOURNAL   mBio. 2021 Mar 23;12(2). pii: mBio.03142-20. doi: 
            10.1128/mBio.03142-20.
  PUBMED    33758083
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mBio."; 
            date: "2021-03-23"; volume: "12(2). pii: mBio.03142-20. doi"; pages:
            " 10.1128/mBio.03142-20"
COMMENT     Sequence Label: pSICO-CPSF6-iRFP670
FEATURES             Location/Qualifiers
     source          1..9595
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1067..1300
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1485..1529
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1827..1944
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2234..2254
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(2272..2305)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        2405..2708
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2709..2912
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      4766..4775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4772..5707
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     protein_bind    complement(5716..5749)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     5776..5792
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    5867..6455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5920..5940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6338..6349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6458..6474)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6984..7164
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7226..7814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7306..7325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7985..8776,8777..8845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8608..8627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8846..8950)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9025..9044)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9216..9595
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
      961 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     1021 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     1081 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     1141 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     1201 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     1261 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     1321 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     1381 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     1441 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     1501 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     1561 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     1621 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     1681 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     1741 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
     1801 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     1861 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     1921 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     1981 ttagtaccgg gcccgctcta gagatccgac gcgccatctc taggcccgcg ccggccccct
     2041 cgcacagact tgtgggagaa gctcggctac tcccctgccc cggttaattt gcatataata
     2101 tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg ttctttttaa
     2161 tactagctac attttacatg ataggcttgg atttctataa gagatacaaa tactaaatta
     2221 ttattttaaa aaacagcaca aaaggaaact caccctaact gtaaagtaat tataacttcg
     2281 tatagtataa attatacgaa gttataagcc ttggtttttt gaattccgta ttaccgccat
     2341 gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     2401 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     2461 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     2521 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     2581 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     2641 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     2701 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     2761 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     2821 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     2881 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcgg atcccagtgt
     2941 ggtggtacgg gaattcagga agatggcgga cggcgtggac cacatagaca tttacgcgga
     3001 tgtcggcgaa gagttcaacc aggaagctga atatggtggg catgatcaga tagatttgta
     3061 tgacgatgtc atatctccat ctgcaaataa tggagatgcc ccagaagacc gagattacat
     3121 ggatactctc ccaccaactg ttggtgatga tgtgggtaaa ggagcagcac caaatgttgt
     3181 ctatacatat actggaaaga gaattgcatt atatattgga aatctaacat ggtggacaac
     3241 agatgaagac ttaactgaag cagttcattc tttgggagta aatgatattt tggagataaa
     3301 attttttgaa aatcgggcaa atggccagtc aaaggggttt gcccttgttg gtgttggatc
     3361 tgaagcatct tcaaaaaagt taatggatct gttacctaaa agagaacttc atggtcagaa
     3421 tcctgttgta actccatgca ataaacagtt cctgagtcaa tttgaaatgc agtccaggaa
     3481 aactacacaa tcaggacaaa tgtctgggga aggtaaagct ggtcctccag gaggcagttc
     3541 ccgtgcagca tttccacaag gtggtagagg acggggccgt tttccagggg ctgttcctgg
     3601 tggggacaga tttcctgggc cagcaggacc aggagggcca cccccacctt ttccaggaaa
     3661 tttgatcaag catcttgtta aaggaactcg gcctttgttc ctggaaacta ggattccatg
     3721 gcatatgggg cacagcatag aggaaatacc catttttggc ctaaaagctg gacagactcc
     3781 accacgtcca cccttaggtc ctccaggccc acctggtcca ccaggtcctc cacctcctgg
     3841 tcaggttctg cctcctcctc tagctgggcc tcctaatcga ggagatcgcc ctccaccacc
     3901 agttcttttt cctggacaac cttttgggca gcctccattg ggtccacttc ctcctggccc
     3961 tccacctcca gttccaggct acggcccccc tcctggccca ccacctccac aacagggacc
     4021 acctccacct ccaggcccct ttccacctcg tccacccggt ccacttgggc caccccttac
     4081 actagctcct cctccgcatc ttcctggacc acctccaggt gccccaccgc cagctccgca
     4141 tgtgaaccca gctttctttc ctccaccaac taacagtggc atgcctacat cagatagccg
     4201 aggtccacca ccaacagatc catatgggcg acctccacca tatgataggg gtgactatgg
     4261 cccccctgga agggaaatgg atactgcaag aacgccattg agtgaagctg aatttgaaga
     4321 aatcatgaat agaaataggg caatctcaag cagtgctatt tcgagagctg tgtctgatgc
     4381 cagtgctggt gattatggga gtgctattga gacactggta actgcaattt ctttaattaa
     4441 acaatccaaa gtatctgctg atgatcgttg caaagttctt attagttctt tgcaagattg
     4501 ccttcatgga attgagtcca agtcttatgg ttctggatca agacgtgaac gatcaagaga
     4561 gagggaccat agtagatcac gagaaaagag tcgacgtcat aaatcccgta gtagagaccg
     4621 tcatgacgat tattacagag agagaagcag agaacgagag aggcaccggg atcgtgaccg
     4681 agaccgtgac cgagagcgtg accgagagcg cgaatatcgt catcgtgagg taccgcgggc
     4741 ccgggatccg ctagcgctac cggtcgccac catggcgcgt aaggtcgatc tcacctcctg
     4801 cgatcgcgag ccgatccaca tccccggcag cattcagccg tgcggctgcc tgctagcctg
     4861 cgacgcgcag gcggtgcgga tcacgcgcat tacggaaaat gccggcgcgt tctttggacg
     4921 cgaaactccg cgggtcggtg agctactcgc cgattacttc ggcgagaccg aagcccatgc
     4981 gctgcgcaac gcactggcgc agtcctccga tccaaagcga ccggcgctga tcttcggttg
     5041 gcgcgacggc ctgaccggcc gcaccttcga catctcactg catcgccatg acggtacatc
     5101 gatcatcgag ttcgagcctg cggcggccga acaggccgac aatccgctgc ggctgacgcg
     5161 gcagatcatc gcgcgcacca aagaactgaa gtcgctcgaa gagatggccg cacgggtgcc
     5221 gcgctatctg caggcgatgc tcggctatca ccgcgtgatg ttgtaccgct tcgcggacga
     5281 cggctccggg atggtgatcg gcgaggcgaa gcgcagcgac ctcgagagct ttctcggtca
     5341 gcactttccg gcgtcgctgg tcccgcagca ggcgcggcta ctgtacttga agaacgcgat
     5401 ccgcgtggtc tcggattcgc gcggcatcag cagccggatc gtgcccgagc acgacgcctc
     5461 cggcgccgcg ctcgatctgt cgttcgcgca cctgcgcagc atctcgccct gccatctcga
     5521 atttctgcgg aacatgggcg tcagcgcctc gatgtcgctg tcgatcatca ttgacggcac
     5581 gctatgggga ttgatcatct gtcatcatta cgagccgcgt gccgtgccga tggcgcagcg
     5641 cgtcgcggcc gaaatgttcg ccgacttctt atcgctgcac ttcaccgccg cccaccacca
     5701 acgctaagcg gccgcataac ttcgtatagt ataaattata cgaagttata agccttgtta
     5761 acgcgcggtg accctcgagg tcgacggtat cgataagctc gcttcacgag atcatgttta
     5821 agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga
     5881 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5941 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6001 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     6061 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     6121 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     6181 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     6241 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     6301 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     6361 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     6421 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     6481 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     6541 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     6601 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6661 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     6721 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6781 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6841 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6901 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6961 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7021 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7081 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7141 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     7201 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     7261 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     7321 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7381 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7501 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7561 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7621 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7681 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7741 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7801 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7861 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7921 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7981 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     8041 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     8101 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     8161 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     8221 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     8281 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     8341 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8401 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8461 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8521 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8581 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8641 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8701 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8761 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8821 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8881 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8941 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     9001 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     9061 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     9121 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     9181 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     9241 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     9301 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     9361 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     9421 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     9481 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     9541 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
