LOCUS       sequence-330875-        9134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9134)
  AUTHORS   Chen L, Roake CM, Freund A, Batista PJ, Tian S, Yin YA, Gajera CR, 
            Lin S, Lee B, Pech MF, Venteicher AS, Das R, Chang HY, Artandi SE
  TITLE     An Activity Switch in Human Telomerase Based on RNA Conformation and
            Shaped by TCAB1.
  JOURNAL   Cell. 2018 Jun 28;174(1):218-230.e13. doi: 
            10.1016/j.cell.2018.04.039. Epub 2018 May 24.
  PUBMED    29804836
REFERENCE   2  (bases 1 to 9134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.04.039"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "218-230.e13"
COMMENT     Sequence Label: pCDH-3xFLAG-hTCAB1-puroR
FEATURES             Location/Qualifiers
     source          1..9134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1691
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..1984
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1935..1955
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1981..2005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2085..2150
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3862..4073
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4086..4354
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4381..4980
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4381..4400)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4877..4897
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4987..5575
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5040..5060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5458..5469)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5649..5882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5954..6075
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5991..6010)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6045..6064
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6277..6293)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6277..6293)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6290..6312)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6301..6317
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6325..6331,6332..6349,6350..6355))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6370..6391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6508..6525)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6679..7267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7438..8229,8230..8298))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8061..8080
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8299..8403)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8471..8489
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8527..8549)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8649..8668
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8862..8884
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8876..8893
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8877..8893
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8908..9134
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 ttcaaaattt tatcgatact agtattatgc ccagtacatg accttatggg actttcctac
     1741 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     1801 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     1861 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     1921 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag
     1981 agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
     2041 tagaagattc tagagctagc gaattcacca tggggtcccg ggctgactac aaagaccatg
     2101 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag gggcgcgcca
     2161 tgaagacttt ggagactcaa ccgttagctc cggactgctg tccttcagac caggacccag
     2221 ctccagccca tccttctccc cacgcttccc cgatgaataa aaatgcggac tctgaactga
     2281 tgccaccgcc tcccgaaagg ggggatccgc cccggttgtc cccagatcct gtggctggct
     2341 cagctgtgtc ccaggagcta cgggaggggg acccagtttc tctctccact cccctggaaa
     2401 cagagtttgg ttcccctagt gagttgagtc ctcgaatcga ggagcaagaa ctttctgaaa
     2461 atacaagcct tcctgcagaa gaagcaaacg ggagcctttc tgaagaagaa gcgaacgggc
     2521 cagagttggg gtctggaaaa gccatggaag atacctctgg ggaacccgct gcagaggacg
     2581 agggagacac cgcttggaac tacagcttct cccagctgcc tcgatttctc agtggttcct
     2641 ggtcagagtt cagcacccaa cctgagaact tcttgaaagg ctgtaagtgg gctcctgacg
     2701 gttcctgcat cttgaccaat agtgctgata acatcttgcg aatttataac ctgcccccag
     2761 agctgtacca tgagggggag caggtggaat atgcagaaat ggtccctgtc cttcgaatgg
     2821 tggaaggtga taccatctat gattactgct ggtattctct gatgtcctca gcccagccag
     2881 acacctccta cgtggccagc agcagccggg agaacccgat tcatatctgg gacgcattca
     2941 ctggagagct ccgggcttcc tttcgcgcct acaaccacct ggatgagctg acggcagccc
     3001 attcgctctg cttctccccg gatggctccc agctcttctg tggcttcaac cggactgtgc
     3061 gtgttttttc cacggcccgg cctggccgag actgcgaggt ccgagccaca tttgcaaaaa
     3121 agcagggcca gagcggcatc atctcctgca tagccttcag cccagcccag cccctctatg
     3181 cctgtggctc ctacggccgc tccctgggtc tgtatgcctg ggatgatggc tcccctctcg
     3241 ccttgctggg agggcaccaa gggggcatca cccacctctg ctttcatccc gatggcaacc
     3301 gcttcttctc aggagcccgc aaggatgctg agctcctgtg ctgggatctc cggcagtctg
     3361 gttacccact gtggtccctg ggtcgagagg tgaccaccaa tcagcgcatc tacttcgatc
     3421 tggacccgac cgggcagttc ctagtgagtg gcagcacgag cggggctgtc tctgtgtggg
     3481 acacggacgg gcctggcaat gatgggaagc cggagcccgt gttgagtttt ctgccccaga
     3541 aggactgcac caatggcgtg agcctgcacc ctagcctgcc tctcctggcc actgcctccg
     3601 gtcagcgtgt gtttcctgag cccacagaga gtggggacga aggagaggag ctgggccttc
     3661 ccttgctctc cacgcgccac gtccaccttg aatgtcggct tcagctctgg tggtgtgggg
     3721 gggcgccaga ctccagcatc cctgatgatc accagggcga gaaagggcag ggaggaacgg
     3781 agggaggtgt gggtgagctg atatgattaa ttaaggatcc gcggccgcga aggatctgcg
     3841 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     3901 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa
     3961 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     4021 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc
     4081 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac
     4141 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg
     4201 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc
     4261 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct
     4321 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag
     4381 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     4441 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     4501 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     4561 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     4621 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     4681 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     4741 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     4801 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     4861 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     4921 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     4981 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5041 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5101 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5161 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5221 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5281 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5341 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     5401 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5461 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5521 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt
     5581 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5641 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     5701 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5761 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5821 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5881 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5941 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6001 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6061 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     6121 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6181 tatgcagagg ccgaggcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6241 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
     6301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8281 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8341 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8401 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8461 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     8521 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8581 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     8641 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8701 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     8761 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8821 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8881 aacgacggcc agtgccaagc tgacgcgtgt agtcttatgc aatactcttg tagtcttgca
     8941 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     9001 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     9061 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     9121 ctcgatacat aaac
//
