LOCUS       sequence-331069-        1915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression of mouse SPOP F133V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Bouchard JJ, Otero JH, Scott DC, Szulc E, Martin EW, Sabri N, 
            Granata D, Marzahn MR, Lindorff-Larsen K, Salvatella X, Schulman BA,
            Mittag T
  TITLE     Cancer Mutations of the Tumor Suppressor SPOP Disrupt the Formation 
            of Active, Phase-Separated Compartments.
  JOURNAL   Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi: 
            10.1016/j.molcel.2018.08.027. Epub 2018 Sep 20.
  PUBMED    30244836
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.08.027"; journalName: "Mol Cell"; date: 
            "2018-10-4- 4"; volume: "72"; issue: "1"; pages: "19-36.e8"
COMMENT     Sequence Label: SPOP-V5 (F133V)
FEATURES             Location/Qualifiers
     source          1..1915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     100..119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        100..118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             208..249
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(1406..1423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1412..1636
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1769..1788)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
       61 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      121 gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc cactagtcca
      181 gtgtggtgga attctgcaga tatcatgggt aagcctatcc ctaaccctct cctcggtctc
      241 gattctacga tgtcaagggt tccaagtcct ccacctccgg cagaaatgtc gagtggtccc
      301 gttgccgaga gctggtgcta tacacagatc aaggtagtga agttctccta catgtggacc
      361 atcaataact ttagtttttg ccgggaggaa atgggtgaag tcataaaaag ttcaactttt
      421 tcatcaggag ccaatgataa actgaaatgg tgtttgcgag taaacccgaa agggctagat
      481 gaagaaagca aagattacct gtcgctttac ctgttgttgg tcagctgtcc aaagagtgaa
      541 gttcgggcaa agttcaagtt ctccatcctg aatgccaagg gagaagaaac caaagctatg
      601 gagagtcagc gagcttatag gttcgtgcaa ggcaaagact ggggagtgaa gaaattcatc
      661 cgcagagatt ttctcttgga cgaggccaat gggcttctcc ctgatgacaa gctcaccctc
      721 ttctgtgagg tgagtgtagt acaagactct gtcaatattt ctgggcagaa tactatgaac
      781 atggtaaagg ttcctgaatg ccggttggca gatgagttag gagggctgtg ggagaactct
      841 cgattcaccg actgctgctt gtgtgtggct ggtcaagaat tccaggctca caaggctatc
      901 ttagcagctc ggtctccagt cttcagtgcc atgtttgaac atgaaatgga ggagagcaaa
      961 aagaatcggg ttgagattaa cgacgtggag cctgaggtgt ttaaggagat gatgtgcttc
     1021 atctacacgg ggaaggcgcc gaacctcgac aagatggctg acgacttgct ggcagctgcc
     1081 gataagtacg ccctggagcg tctgaaggtc atgtgtgagg acgccctctg cagtaacctc
     1141 tctgtggaga acgctgcaga gattctcatc ctggctgacc tccacagcgc agatcagctg
     1201 aagacgcagg cagttgattt catcaactat catgcttcgg atgtcttgga gacctctggg
     1261 tggaagtcaa tggtggtgtc acatccccac ttagtggcag aagcataccg ttctctggct
     1321 tcagcacagt gccctttcct ggggccccca cgcaaacgcc tgaagcaatc ctaatctaga
     1381 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     1441 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1501 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     1561 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     1621 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     1681 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     1741 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     1801 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     1861 agtgctttac ggcacctcga ccccaaaaaa cttgattngg gtgatggttc acgta
//
