LOCUS       sequence-331080-         498 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression of human hnRNPA1-A Low complexity domain 
            including amino acids 186-320, aromatic amino acids. PHE and TYR 
            amino acids shuffled to optimize mixing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Martin EW, Holehouse AS, Peran I, Farag M, Incicco JJ, Bremer A, 
            Grace CR, Soranno A, Pappu RV, Mittag T
  TITLE     Valence and patterning of aromatic residues determine the phase 
            behavior of prion-like domains.
  JOURNAL   Science. 2020 Feb 7;367(6478):694-699. doi: 10.1126/science.aaw8653.
  PUBMED    32029630
REFERENCE   2  (bases 1 to 498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-02-7- 7"; volume: "367"; issue: 
            "6478"; pages: "694-699"
COMMENT     Sequence Label: A1-LCD Aro+
FEATURES             Location/Qualifiers
     source          1..498
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    40..64
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             67..87
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgtcgtact accatcacca tcaccatcac ctcgaatcaa caagtttgta caaaaaagca
       61 ggcttcgaaa acctgtattt tcagggtagc atggcatttg caagcagctt tcagcgtggt
      121 cgttatggta gcggtaattt tggtggtggt cgcggtggtg gttttggtgg caatgataat
      181 tttggccgtg gtggtaattt tagcggtcgt ggtggctttg gcggtagccg tggcggtggc
      241 ggttatggcg gtagtggtga tggttataat ggctttggta atgatggtag caactttgga
      301 ggtggtggta gctataatga tttcggcaat tataacaacc agagcagcaa ttttggtccg
      361 atgaaaggtg gcaatttcgg tggtcgtagc agcggtggtt catatggtgg tggccagtat
      421 tttgccaaac cgcgtaatca aggtggctat ggtggtagta gctttagcag cagctatggt
      481 agtggtcgtc gtttttaa
//
