LOCUS       sequence-331114-       10133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Block 2 for AdenoBuilder genome assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Miciak JJ, Hirshberg J, Bunz F
  TITLE     Seamless assembly of recombinant adenoviral genomes from high-copy 
            plasmids.
  JOURNAL   PLoS One. 2018 Jun 27;13(6):e0199563. doi: 
            10.1371/journal.pone.0199563. eCollection 2018.
  PUBMED    29949649
REFERENCE   2  (bases 1 to 10133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-06-27"; volume: "13(6):e0199563. doi"; pages: " 
            10.1371/journal.pone.0199563. eCollection 2018"
COMMENT     Sequence Label: pAd5-B2
FEATURES             Location/Qualifiers
     source          1..10133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..324
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(4112..4149,4150..4215)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     promoter        complement(join(7965..7971,7972..7989,7990..7995))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    8010..8031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8151..8168)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8322..8910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8402..8421)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9081..9872,9873..9941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9704..9723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9942..10046)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10114..10132
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tgttcgaacg agccacttaa tgctttcgct ttccagccta accgcttacg
      421 ctgcgcgcgg ccagtggcca aaaaagctag cgcagcagcc gccgcgcctg gaaggaagcc
      481 aaaaggagca ctcccccgtt gtctgacgtc gcacacctgg gttcgacacg cgggcggtaa
      541 ccgcatggat cacggcggac ggccggatac ggggctcgaa ccccggtcgt ccgccatgat
      601 acccttgcga atttatccac cagaccacgg aagagtgccc gcttacaggc tctccttttg
      661 cacggtctag agcgtcaacg attgcgcgcg cctgaccggc cagagcgtcc cgaccatgga
      721 gcactttttg ccgctgcgca acatctggaa ccgcgtccgc gactttccgc gcgcctccac
      781 caccgccgcc ggcatcacct ggatgtccag gtacatctac ggatatcatc gccttatgtt
      841 ggaagatctc gcccccggag ccccggccac cctacgctgg cccctctacc gccagccgcc
      901 gccgcacttt ttggtgggat accagtacct ggtgcggact tgcaacgact acgtatttga
      961 ctcgagggct tactcgcgtc tcaggtacac cgagctctcg cagccgggtc accagaccgt
     1021 taactggtcc gttatggcca actgcactta caccatcaac acgggcgcat accaccgctt
     1081 tgtggacatg gatgacttcc agtctaccct cacgcaggtg cagcaggcca tattagccga
     1141 gcgcgttgtc gccgacctag ccctgcttca gccgatgagg ggcttcgggg tcacacgcat
     1201 gggaggaaga gggcgccacc tacggccaaa ctccgccgcc gccgcagcga tagatgcaag
     1261 agatgcagga caagaggaag gagaagaaga agtgccggta gaaaggctca tgcaagacta
     1321 ctacaaagac ctgcgccgat gtcaaaacga agcctggggc atggccgacc gcctgcgcat
     1381 tcagcaggcc ggacccaagg acatggtgct tctgtcgacc atccgccgtc tcaagaccgc
     1441 ctactttaat tacatcatca gcagcacctc cgccagaaac aaccccgacc gccgcccgct
     1501 gccgcccgcc acggtgctca gcctaccttg cgactgtgac tggttagacg cctttctcga
     1561 gaggttttcc gatccggtcg atgcggactc gctcaggtcc ctcggcggcg gagtacctac
     1621 acaacaattg ttgagatgca tcgttagcgc cgtatccctg ccgcatggca gccccccgcc
     1681 aacccataac cgggacatga cgggcggcgt cttccaactg cgcccccgcg agaacggccg
     1741 cgccgtcacc gagaccatgc gccgtcgccg cggggagatg atcgagcgct ttgtcgaccg
     1801 cctcccggtg cgccgtcgtc gccgccgtgt cccccctccc ccaccgccgc cagaagaaga
     1861 agaaggggag gcccttatgg aagaggagat tgaagaagaa gaagaggccc ctgtagcctt
     1921 tgagcgcgag gtgcgcgaca ctgtcgccga gctcatccgt cttctggagg aggagttaac
     1981 cgtgtcggcg cgcaactccc agtttttcaa cttcgccgtg gacttctacg aggccatgga
     2041 gcgccttgag gccttggggg atatcaacga atccacgttg cgacgctggg ttatgtactt
     2101 cttcgtggca gaacacaccg ccaccaccct caactacctc tttcagcgcc tgcgaaacta
     2161 cgccgtcttc gcccggcacg tggagctcaa tctcgcgcag gtggtcatgc gcgcccgcga
     2221 tgccgaaggg ggcgtggtct acagccgcgt ctggaacgag ggaggcctca acgccttctc
     2281 gcagctcatg gcccgcattt ccaacgacct cgccgccacc gtggagcgag ccggacgcgg
     2341 agatctccag gaggaagaga tcgagcagtt catggccgag atcgcctatc aagacaactc
     2401 aggagacgtg caggagattt tgcgccaggc cgccgtcaac gacaccgaaa ttgattctgt
     2461 cgaactctct ttcaggttca agctcaccgg gcccgtcgtc ttcacgcaga ggcgccagat
     2521 tcaggagatc aaccgccgcg tcgtcgcgtt cgccagcaac ctacgcgcgc agcaccagct
     2581 cctgcccgcg cgcggcgccg acgtgcccct gccccctctc ccggcgggtc cggagccccc
     2641 cctacctccg ggggctcgcc cgcgtcaccg cttttagatg catcatccaa ggacaccccc
     2701 gcggcccacc gcccgccgcg cggtaccgta gtcgcgccgc ggggatgcgg cctcttgcaa
     2761 gccatcgacg ccgccaccaa ccagcccctg gaaattaggt atcacctgga tctagcccgc
     2821 gccctgaccc gtctatgcga ggtaaacctg caggagctcc cgcctgacct gacgccgcgg
     2881 gagctccaga ccatggacag ctcccatctg cgcgatgttg tcatcaagct ccgaccgccg
     2941 cgcgcggaca tctggacttt gggctcgcgc ggcgtggtgg tccgatccac cgtaactccc
     3001 ctcgagcagc cagacggtca aggacaagca gccgaagtag aagaccacca gccaaacccg
     3061 ccaggcgagg ggctcaaatt cccactctgc ttccttgtgc gcggtcgtca ggtcaacctc
     3121 gtgcaggatg tacagcccgt gcaccgctgc cagtactgcg cacgttttta caaaagccag
     3181 cacgagtgtt cggcccgtcg cagggacttc tactttcacc acatcaatag ccactcctcc
     3241 aattggtggc gggagatcca gttcttcccg atcggctcgc atcctcgcac cgagcgtctc
     3301 tttgtcacct acgatgtaga gacctatact tggatggggg cctttgggaa gcagctcgtg
     3361 cccttcatgc tggtcatgaa gttcggcgga gatgagcctc tagtgactgc cgcgcgagac
     3421 ctagccgcga accttggatg ggaccgctgg gaacaagacc cgcttacctt ctactgcatc
     3481 accccagaaa aaatggccat aggtcgccag tttaggacct ttcgcgacca cctgcaaatg
     3541 ctaatggccc gtgacctgtg gagctcattc gtcgcttcca accctcatct tgcagactgg
     3601 gccctttcag agcacgggct cagctcccct gaagagctca cctacgagga acttaaaaaa
     3661 ttgccttcca tcaagggcat cccgcgcttc ttggaacttt acattgtggg ccacaacatc
     3721 aacggctttg acgagatcgt gctcgccgcc caggtaatta acaaccgttc cgaggtgccg
     3781 ggacccttcc gcatcacacg caactttatg cctcgcgcgg gaaagatact cttcaacgat
     3841 gtcaccttcg ccctgccaaa tccgcgttcc aaaaagcgca cggacttttt gctctgggag
     3901 cagggcggat gcgacgacac tgacttcaaa taccagtacc tcaaagtcat ggtcagggac
     3961 acctttgcgc tcacccacac ctcgctccgg aaggccgcgc aggcatacgc gctacccgta
     4021 gaaaagggat gctgcgccta ccaggccgtc aaccagttct acatgctagg ctcttaccgt
     4081 tcggaggccg acgggtttcc gatccaagag tactggaaag accgcgaaga gtttgtcctc
     4141 aaccgcgagc tgtggaaaaa aaagggacag gataagtatg acatcatcaa ggaaaccctg
     4201 gactactgcg ccctagacgt gcaggtcacc gccgagctgg tcaacaagct gcgcgactcc
     4261 tacgcctcct tcgtgcgtga cgcggtaggt ctcacagacg ccagcttcaa cgtcttccag
     4321 cgtccaacca tatcatccaa ctcacatgcc atcttcaggc agatagtctt ccgagcagag
     4381 cagcccgccc gtagcaacct cggtcccgac ctcctcgctc cctcgcacga actatacgat
     4441 tacgtgcgcg ccagcatccg cggtggaaga tgctacccta catatcttgg aatactcaga
     4501 gagcccctct acgtttacga catttgcggc atgtacgcct ccgcgctcac ccaccccatg
     4561 ccatggggtc ccccactcaa cccatacgag cgcgcgcttg ccgcccgcgc atggcagcag
     4621 gcgctagact tgcaaggatg caagatagac tacttcgacg cgcgcctgct gcccggggtc
     4681 tttaccgtgg acgcagaccc cccggacgag acgcagctag accccctacc gccattctgc
     4741 tcgcgcaagg gcggccgcct ctgctggacc aacgagcgcc tacgcggaga ggtagccacc
     4801 agcgttgacc ttgtcaccct gcacaaccgc ggttggcgcg tgcacctggt gcccgacgag
     4861 cgcaccaccg tctttcccga atggcggtgc gttgcgcgcg aatacgtgca gctaaacatc
     4921 gcggccaagg agcgcgccga tcgcgacaaa aaccaaaccc tgcgctccat cgccaagttg
     4981 ctgtccaacg ccctctacgg gtcgtttgcc accaagcttg acaacaaaaa gattgtcttt
     5041 tctgaccaga tggatgcggc caccctcaaa ggcatcaccg cgggccaggt gaatatcaaa
     5101 tcctcctcgt ttttggaaac tgacaatctt agcgcagaag tcatgcccgc ttttcagagg
     5161 gagtactcac cccaacagct ggccctcgca gacagcgatg cggaagagag tgaggacgaa
     5221 cgcgccccca ccccctttta tagcccccct tcaggaacac ccggtcacgt ggcctacacc
     5281 tacaaaccaa tcaccttcct tgatgccgaa gagggcgaca tgtgtcttca caccctggag
     5341 cgagtggacc ccctagtgga caacgaccgc tacccctccc acttagcctc cttcgtgctg
     5401 gcctggacgc gagcctttgt ctcagagtgg tccgagtttc tatacgagga ggaccgcgga
     5461 acaccgctcg aggacaggcc tctcaagtct gtatacgggg acacggacag ccttttcgtc
     5521 accgagcgtg gacaccggct catggaaacc agaggtaaga aacgcatcaa aaagcatggg
     5581 ggaaacctgg tttttgaccc cgaacggcca gagctcacct ggctcgtgga atgcgagacc
     5641 gtctgcgggg cctgcggcgc ggatgcctac tccccggaat cggtatttct cgcgcccaag
     5701 ctctacgccc tcaaaagtct gcactgcccc tcgtgcggcg cctcctccaa gggcaagctg
     5761 cgcgccaagg gccacgccgc ggaggggctg gactatgaca ccatggtcaa atgctacctg
     5821 gccgacgcgc agggcgaaga ccggcagcgc ttcagcacca gcaggaccag cctcaagcgc
     5881 accctggcca gcgcgcagcc cggagcgcac cccttcaccg tgacccagac tacgctgacg
     5941 aggaccctgc gcccgtggaa agacatgacc ctggcccgtc tggacgagca ccgactactg
     6001 ccgtacagcg aaagccgccc caacccgcga aacgaggaga tatgctggat cgagatgccg
     6061 tagagcaggt gaccgagctg tgggaccgcc tggaactgct tggtcaaacg ctcaaaagca
     6121 tgcctacggc ggacggtctc aaaccgttga aaaactttgc ttccttgcaa gaactgctat
     6181 cgctgggcgg cgagcgcctt ctggcggatt tggtcaggga aaacatgcga gtcagggaca
     6241 tgcttaacga agtggccccc ctgctcaggg atgacggcag ctgcagctct cttaactacc
     6301 agttgcagcc ggtaataggt gtgatttacg ggcccaccgg ctgcggtaag tcgcagctgc
     6361 tcaggaacct gctttcttcc cagctgatct cccctacccc ggaaaccgtt ttcttcatcg
     6421 ccccgcaggt agacatgatc cccccatctg aactcaaagc gtgggaaatg caaatctgtg
     6481 agggtaacta cgcccctggg ccggatggaa ccattatacc gcagtctggc accctccgcc
     6541 cgcgctttgt aaaaatggcc tatgacgatc tcatcctgga acacaactat gacgttagtg
     6601 atcccagaaa tatcttcgcc caggccgccg cccgtgggcc cattgccatc attatggacg
     6661 aatgcatgga aaatcttgga ggtcacaagg gcgtctccaa gttcttccac gcatttcctt
     6721 ctaagctaca tgacaaattt cccaagtgca ccggatacac tgtgctggtg gttctgcaca
     6781 acatgaatcc ccggagggat atggctggga acatagccaa cctaaaaata cagtccaaga
     6841 tgcatctcat atccccacgt atgcacccat cccagcttaa ccgctttgta aacacttaca
     6901 ccaagggcct gcccctggca atcagcttgc tactgaaaga catttttagg caccacgccc
     6961 agcgctcctg ctacgactgg atcatctaca acaccacccc gcagcatgaa gctctgcagt
     7021 ggtgctacct ccaccccaga gacgggctta tgcccatgta tctgaacatc cagagtcacc
     7081 tttaccacgt cctggaaaaa atacacagga ccctcaacga ccgagaccgc tggtcccggg
     7141 cctaccgcgc gcgcaaaacc cctaaataaa gacagcaaga cacttgcttg atccaaatcc
     7201 aaacagagtc tggtttttta tttatgtttt aaaccgcatt gggaggggag gaagccttca
     7261 gggcagaaac ctgctggcgc agatccaaca gctgctgaga aacgacatta agttcccggg
     7321 tcaaagaatc caattgtgcc aaaagagccg tcaacttgtc atcgcgggcg gatgaacggg
     7381 aagctgcact gcttgcaagc gggctcagga aagcaaagtc agtcacaatc ccgcgggcgg
     7441 tggctgcagc ggctgaagcg gcggcggagg ctgcagtctc caacggcgtt ccagacacgg
     7501 tctcgtaggt caaggtagta gagttcgaag atctttctag aagatctcct acaatattct
     7561 cagctgccat ggaaaatcga tgttcttctt ttattctctc aagattttca ggctgtatat
     7621 taaaacttat attaagaact atgctaacca cctcatcagg aaccgttgta ggtggcgtgg
     7681 gttttcttgg caatcgactc tcatgaaaac tacgagctaa atattcaata tgttcctctt
     7741 gaccaacttt attctgcatt ttttttgaac gaggtttaga gcaagcttca ggaaactgag
     7801 acaggaattt tattaaaaat ttaaattttg aagaaagttc agggttaata gcatccattt
     7861 tttgctttgc aagttcctca gcattcttaa caaaagacgt ctcttttgac atgtttaaag
     7921 tttaaacctc ctgtgtgaaa ttattatccg ctcataattc cacacattat acgagccgga
     7981 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8041 cgctcactgc caattgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8101 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8161 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8221 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8281 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8341 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8401 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8461 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8521 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8581 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8641 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8701 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8761 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8821 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8881 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8941 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9001 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9061 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9121 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9181 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9241 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9301 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9361 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9421 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9481 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9541 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9601 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9661 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9721 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9781 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9841 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9901 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9961 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10021 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    10081 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gcc
//
