LOCUS       sequence-331187-        1114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression of cytoplasmic tail of mouse beta3 integrin with 
            N-terminal GST fusion protein in bacterial cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Zhang P, Azizi L, Kukkurainen S, Gao T, Baikoghli M, Jacquier MC, 
            Sun Y, Maatta JAE, Cheng RH, Wehrle-Haller B, Hytonen VP, Wu J
  TITLE     Crystal structure of the FERM-folded talin head reveals the 
            determinants for integrin binding.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Dec 22;117(51):32402-32412. doi: 
            10.1073/pnas.2014583117. Epub 2020 Dec 7.
  PUBMED    33288722
REFERENCE   2  (bases 1 to 1114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2014583117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-12-22- 22"; volume: "117"; issue: "51"; pages: 
            "32402-32412"
COMMENT     Sequence Label: WT-beta-3
FEATURES             Location/Qualifiers
     source          1..1114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     188..206
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     351..373
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(574..591)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(618..640)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 gnatnttgnt actcatactc ttccttttnn ntattattga agcatttatc agggntattg
       61 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
      121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
      181 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattataca ctccgctatc
      241 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
      301 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
      361 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagatcg tcagtcagtc
      421 acgatgaatt caagtccccc ggtaggtgat attggtgaag gtggaggtgg cctctttata
      481 cagcgggttg tttgctgtgt cccacttggc tctggctcgt tcttcctcaa atttagcaaa
      541 ttccttccgg tcatgaatgg tgatgagtag cttggatcca cgcggaacca gatccgattt
      601 tggaggatgg tcgccaccac caaacgtggc ttgccagccc tgcaaaggcc atgctatata
      661 cttgctggat ttcaagtact tatcaatttg tgggatagct tcaatacgtt ttttaaaaca
      721 aactaatttt gggaacgcat ccaggcacat tgggtccatg tataanacaa catcaagagc
      781 gtcatacaac atgaagtcag gatgggttac atgatcacca tttaaatatg ttttatgaca
      841 tanacgatct tcgaacattt tcagcatttc aggtagcttg ctaagaaaat caactttgag
      901 agtttcaaag tctttactat atgcaattct cgaaacaccg tatctaatat ccaaaaccgc
      961 tccttcaagc attgaaatct ctgcacgctc ttttggacaa ccacncaaca tgttgngctt
     1021 gtcagctata taacgtatga tggccataga ctgngttaat ttaacatcnc catcaatata
     1081 ataaggaaga ttgggaaact ccaaacccaa ttca
//
