LOCUS       sequence-331238-        3735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-353c-3X
FEATURES             Location/Qualifiers
     source          1..3735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(323..339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(323..339)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(336..358)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    347..363
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(371..377,378..395,396..401))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    416..437
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(554..571)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(725..1313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(805..824)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1484..2275,2276..2344))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2107..2126
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2345..2449)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2517..2535
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2573..2595)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2695..2714
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2908..2930
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2922..2939
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2923..2939
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctctagcacc gcttaaacgc acgtacgcgc tgtctaccgc gttttaaccg ccactagaag
       61 cgcttactag tctccaggca cgtgtgagac cggcacatga aaaaaaaaag catgctcgag
      121 tatgaaaaaa aaaacgcatc ccggtgccga ggccgctcaa ttggtcgtag acagctctag
      181 caccgcttaa acgcacgtac gcgctgtcta ccgcgtttta accgccacta gaagcgctta
      241 ctagtctcca ggcacgtgtg agaccggcac atgaaaaaaa aaacgtcagc ggtaccagat
      301 ctccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      361 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
      421 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
      481 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      541 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      721 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1141 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2521 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     2581 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     2641 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     2701 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     2761 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     2821 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     2881 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     2941 aattcgagct cggtacccgg ggatccggta ccgctgacga aaaaaaaaac gcatcccggt
     3001 gccgaggccg ctcaattggt cgtagacagc tctagcaccg cttaaacgca cgtacgcgct
     3061 gtctaccgcg ttttaaccgc cactagaagc gcttactagt ctccaggcac gtgtgagacc
     3121 ggcacatgaa aaaaaaaagc atgctcgagt atgaaaaaaa aaacgcatcc cggtgccgag
     3181 gccgctcaat tggtcgtaga cagctctagc accgcttaaa cgcacgtacg cgctgtctac
     3241 cgcgttttaa ccgccactag aagcgcttac tagtctccag gcacgtgtga gaccggcaca
     3301 tgaaaaaaaa aacgtcagcg gtaccgctga cgaaaaaaaa aacgcatccc ggtgccgagg
     3361 ccgctcaatt ggtcgtagac agctctagca ccgcttaaac gcacgtacgc gctgtctacc
     3421 gcgttttaac cgccactaga agcgcttact agtctccagg cacgtgtgag accggcacat
     3481 gaaaaaaaaa agcatgctcg agtatgaaaa aaaaaacgca tcccggtgcc gaggccgctc
     3541 aattggtcgt agacagctct agcaccgctt aaacgcacgt acgcgctgtc taccgcgttt
     3601 taaccgccac tagaagcgct tactagtctc caggcacgtg tgagaccggc acatgaaaaa
     3661 aaaaacgtca gcggtaccgc tgacgaaaaa aaaaacgcat cccggtgccg aggccgctca
     3721 attggtcgta gacag
//
