LOCUS       sequence-331249-        3690 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Blunt-338b-3X
FEATURES             Location/Qualifiers
     source          1..3690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(138..155)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(138..154)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(147..169)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(363..382)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     482..504
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(542..560)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        628..732
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(733..801,802..1593)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(951..970)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1764..2352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2253..2272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2506..2523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2640..2661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2676..2681,2682..2699,2700..2706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2714..2730
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2719..2741
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2738..2754
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2738..2754
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggcggttaaa acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
       61 gaccaattga gcggcctcgg caccgggatg cgtttttttt ccgcgatatc ggatccccgg
      121 gtaccgagct cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      181 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      241 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
      301 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
      361 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
      421 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
      481 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
      541 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      601 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      661 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      721 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      781 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      841 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      901 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      961 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1021 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1081 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1141 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1201 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1261 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1321 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     1381 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     1441 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     1501 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1561 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1621 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1681 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2521 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2581 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2641 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2701 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2761 tacgccaagc ttggagatct gatatcgcgg ttttttttca tgtgccggtc tcacacgtgc
     2821 ctggagacta gtaagcgctt ctagtggcgg ttaaaacgcg gtagacagcg cgtacgtgcg
     2881 tttaagcggt gctagagctg tctacgacca attgagcggc ctcggcaccg ggatgcgttt
     2941 tttttttgcg ctcctgcttt tttttttcat gtgccggtct cacacgtgcc tggagactag
     3001 taagcgcttc tagtggcggt taaaacgcgg tagacagcgc gtacgtgcgt ttaagcggtg
     3061 ctagagctgt ctacgaccaa ttgagcggcc tcggcaccgg gatgcgtttt ttttccgcga
     3121 tatcgcggtt ttttttcatg tgccggtctc acacgtgcct ggagactagt aagcgcttct
     3181 agtggcggtt aaaacgcggt agacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
     3241 tacgaccaat tgagcggcct cggcaccggg atgcgttttt tttttgcgct cctgcttttt
     3301 tttttcatgt gccggtctca cacgtgcctg gagactagta agcgcttcta gtggcggtta
     3361 aaacgcggta gacagcgcgt acgtgcgttt aagcggtgct agagctgtct acgaccaatt
     3421 gagcggcctc ggcaccggga tgcgtttttt ttccgcgata tcgcggtttt ttttcatgtg
     3481 ccggtctcac acgtgcctgg agactagtaa gcgcttctag tggcggttaa aacgcggtag
     3541 acagcgcgta cgtgcgttta agcggtgcta gagctgtcta cgaccaattg agcggcctcg
     3601 gcaccgggat gcgttttttt tttgcgctcc tgcttttttt tttcatgtgc cggtctcaca
     3661 cgtgcctgga gactagtaag cgcttctagt
//
