LOCUS       sequence-331254-        3718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  DNA fragment production for dinucleosome preparation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey 
            GE, Davey CA
  TITLE     Engineering nucleosomes for generating diverse chromatin assemblies.
  JOURNAL   Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 
            10.1093/nar/gkab070.
  PUBMED    33590100
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070."
COMMENT     Sequence Label: Sticky-357d-3X
FEATURES             Location/Qualifiers
     source          1..3718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(192..208)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(205..227)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    216..232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(240..246,247..264,265..270))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    285..306
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(423..440)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(594..1182)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(674..693)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1353..2144,2145..2213))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1976..1995
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2214..2318)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2386..2404
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2442..2464)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2564..2583
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2777..2799
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2791..2808
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2792..2808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct tctagtggcg
       61 gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct gtctacgacc
      121 aattgagcgg cctcggcacc gggatgcgtt ttttttttcg ttgcagcggt accaagcttg
      181 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      241 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      301 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      361 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      421 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      481 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      541 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      601 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      661 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      721 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      781 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      841 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      901 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      961 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1021 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1081 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1141 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1201 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1261 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1321 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1381 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1441 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1501 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1561 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1621 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1681 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1741 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1801 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1861 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1921 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1981 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2041 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2101 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2161 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2221 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2281 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2341 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2401 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2461 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2521 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2581 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2641 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2701 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2761 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcggtacc
     2821 gcttggacgt tttttttttc atgtgccggt ctcacacgtg cctggagact agtaagcgct
     2881 tctagtggcg gttaaaacgc ggtagacagc gcgtacgtgc gtttaagcgg tgctagagct
     2941 gtctacgacc aattgagcgg cctcggcacc gggatgcgtt ttttttttca tactcgagca
     3001 tgcttttttt tttcatgtgc cggtctcaca cgtgcctgga gactagtaag cgcttctagt
     3061 ggcggttaaa acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
     3121 gaccaattga gcggcctcgg caccgggatg cgtttttttt ttcgttgcag cggtaccgct
     3181 tggacgtttt ttttttcatg tgccggtctc acacgtgcct ggagactagt aagcgcttct
     3241 agtggcggtt aaaacgcggt agacagcgcg tacgtgcgtt taagcggtgc tagagctgtc
     3301 tacgaccaat tgagcggcct cggcaccggg atgcgttttt tttttcatac tcgagcatgc
     3361 tttttttttt catgtgccgg tctcacacgt gcctggagac tagtaagcgc ttctagtggc
     3421 ggttaaaacg cggtagacag cgcgtacgtg cgtttaagcg gtgctagagc tgtctacgac
     3481 caattgagcg gcctcggcac cgggatgcgt tttttttttc gttgcagcgg taccgcttgg
     3541 acgttttttt tttcatgtgc cggtctcaca cgtgcctgga gactagtaag cgcttctagt
     3601 ggcggttaaa acgcggtaga cagcgcgtac gtgcgtttaa gcggtgctag agctgtctac
     3661 gaccaattga gcggcctcgg caccgggatg cgtttttttt ttcatactcg agcatgct
//
