LOCUS       sequence-331312-        1371 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cleavable His-tagged PTPIP51_Delta-TPR domain (36-235) in 
            E. Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Yeo HK, Park TH, Kim HY, Jang H, Lee J, Hwang GS, Ryu SE, Park SH, 
            Song HK, Ban HS, Yoon HJ, Lee BI
  TITLE     Phospholipid transfer function of PTPIP51 at mitochondria-associated
            ER membranes.
  JOURNAL   EMBO Rep. 2021 Jun 4;22(6):e51323. doi: 10.15252/embr.202051323. 
            Epub 2021 May 2.
  PUBMED    33938112
REFERENCE   2  (bases 1 to 1371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Rep.";
            date: "2021-06-4"; volume: "22(6):e51323. doi"; pages: " 
            10.15252/embr.202051323. Epub 2021 May 2"
COMMENT     Sequence Label: pQlinkH-PTPIP51(36-235)
FEATURES             Location/Qualifiers
     source          1..1371
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(44..58,59..64,65..81,82..88)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    64..80
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             131..142
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             155..175
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    188..212
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             212..232
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(852..876)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      898..992
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gcattcgctc tcgagcttat taacaacacc atttgtcgag aaatcataaa aaatttattt
       61 gctttgtgag cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac
      121 agaattcatt aaagaggaga aattaactat gaaacatcac catcaccatc accatggtgc
      181 tgcaggtaca agtttgtaca aaaaagcagg cgagaatctt tattttcagg gatccaaacg
      241 gacccagcgt catggccgca gccagagcct gcccaactcc ctggactata cgcagacttc
      301 agatcccgga cgccacgtga tgctcctgcg ggctgtccca ggtggggctg gagatgcctc
      361 agtgctgccc agccttccac gggaaggaca ggagaaggtg ctggaccgcc tggactttgt
      421 gctgaccagc cttgtggcgc tgcggcggga ggtggaggtg ctgagaagca gcctgcgagg
      481 gcttgcgggg gagattgttg gggaggtccg atgccacatg gaagagaacc agagagtggc
      541 tcggcggcga aggtttccgt ttgtccggga gaggagtgac tccactggct ccagctctgt
      601 ctacttcacg gcctcctcgg gagccacgtt cacagatgct gagagtgaag ggggttacac
      661 aacagccaat gcggagtctg acaatgagcg ggactctgac aaagaaagtg aggacgggga
      721 agatgaagtg agctgtgaga ctgtgaagat ggggagaaag gattctcttg acttggagga
      781 agaggcagct tcaggtgcct ccagtgccct ggaggctgga ggttcctcag gcttgtaggc
      841 ggccgcctag gacccagctt tcttgtacaa agtggtaagc ttaattagct gagcttggac
      901 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg
      961 gttgccgccg ggcgtttttt attggtgaga atccaagtaa caacaccatt taaatggagt
     1021 ggttacaaat ggagtggtta attaaggcta gcttggcgag attttcagga gctaaggaag
     1081 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
     1141 aagaacattt tgaggcattt cagtcagtgg ctcaaggacc tataaccaga ccgttcagct
     1201 ggaattacgg cctttttaag aaccgtaaga aaaaaaaccc aagttttatc cggcctttat
     1261 tcaattcttg ccccccgaga aaggccctcc cgaatttctt tggcatgaaa aacgggaacg
     1321 ggggatgggg aaaggttccc cttgttaccg ttttctggaa aaataaaaat t
//
