LOCUS       sequence-331406-        6172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible expression of phzO for conversion of 
            phenazine-1-carboxylic acid to 2-hydroxyphenazine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6172)
  TITLE     Bioelectronic sensors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSB4A3-PBAD-phzO
FEATURES             Location/Qualifiers
     source          1..6172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..24
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      25..82
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(106..123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(385..1335)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      1383..1605
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(2097..2191)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2221..3012,3013..3081))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2844..2863
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3082..3186)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3251..3269
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3273..3316)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3319..3340
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             complement(3341..4219)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4246..4530
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4453..4472
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             4542..4553
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      6055..6126
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6142..6169
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 atatactagt agcggccgct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc
       61 tttttcttta aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc
      121 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatctc
      181 gaggttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa aactcgtaaa
      241 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
      301 tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
      361 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
      421 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg
      481 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
      541 gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg ttgttggtat
      601 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
      661 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
      721 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
      781 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
      841 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
      901 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
      961 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     1021 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     1081 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     1141 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     1201 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     1261 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     1321 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     1381 atgggctagt caatgataat tacatgtcct tttcctttga gttgtgggta tctgtaaatt
     1441 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     1501 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     1561 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     1621 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     1681 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
     1741 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     1801 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     1861 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
     1921 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
     1981 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
     2041 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
     2101 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     2161 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag
     2221 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2281 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2341 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2401 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2461 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2521 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2581 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2641 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2701 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2761 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2821 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2881 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2941 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3001 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3061 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3121 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3181 tccgcgcaca tttcccatgg tgccacctga cgtctaagaa accattatta tcatgacatt
     3241 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
     3301 cgctaaggat gatttctgga attcgcggcc gcttctagag ttatgacaac ttgacggcta
     3361 catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt
     3421 ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg
     3481 cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc
     3541 gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg
     3601 acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc
     3661 tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa
     3721 tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat
     3781 agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct
     3841 ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc
     3901 attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct
     3961 ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc
     4021 ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga
     4081 gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct
     4141 caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat
     4201 cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc
     4261 catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac
     4321 cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa
     4381 cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg
     4441 tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct
     4501 ttttatcgca actctctact gtttctccat tactagagat taaagaggag aaatactaga
     4561 tgctggattt tcagaataaa cgcaaatatc tgaaaagcgc agaaagcttt aaagcaagcc
     4621 tgcgtgataa tcgtaccgtt atttatcagg gtcaggttgt tgaagatgtg accacccatt
     4681 ttagcaccgc aggcggtatt agccaggttg cagaaatcta tgaagaacag tttagcggtg
     4741 aacacgatga tattctgacc tatgttcgtc cggatggtta tctggcaagc agcgcatata
     4801 tgcctccgcg taataaagaa gatctggcaa gccgtcgtcg tgcaattatg tatgttagcc
     4861 agaaaacctg gggcacccat tgtcgtaatc tggatatgat tgcaagcttt accgttggta
     4921 tgatgggtta tctgccgacc tttcgtaaaa aatgtccgga atatgcagaa aacatcaccg
     4981 agtatcatga ttatgcagaa cgcaatagcc tgtatctgag cgaaaccatt gttgatccgc
     5041 agggttatcg tgcacgtacc catggcaccg atctgaatct gcctccgcct gatcgtgcag
     5101 ttatgcgtat taacaaacag aatgcagaag gcatttggat cagcggtgtt aaaggtgttg
     5161 gcaccgcagc accgcagagc aatgaaattt ttgttggtag cctgtttccg gcagcaccgg
     5221 aagaaagctt ttgggcatat gttccggttg atgcaccggg tgttaaaatc ttttgtcgtg
     5281 aaattgttag ccagccgcat gcaagcgcat atgatcatcc gctgattagc aaaggtgaag
     5341 aggcagaagc aatggtcgtt tttgataatg tttttattcc gcgttggcgt attatggcag
     5401 caaatgttcc ggaactggca agcgcaggtt tttttagcct gtggaccagc tatagccatt
     5461 ggtataccct ggttcgtctg gaaaccaaag cagatctgta tgcaggtctg gcaaaagtta
     5521 ttatggaagt tctgggtctg gaaggtattg cagttgttcg tcagcgtgtt agcgaaattg
     5581 ttcagctggc agaaattctg aaaggtatgt gtattgcaag catcgaaacc gcagaaatga
     5641 gtgatggtga tattctgctg cctggtcata atgcactggc agcaggtcgt gtttttgcaa
     5701 tggaaaaact gcctcgtgtt ctgcatctgc tgcgtgaact gtgtggtcag ggtctgattc
     5761 tgcgttttaa tgagaaagat ctggcagcag atgccgcatt tggtcagaaa tttagctggt
     5821 ttctggatac ccagagcgtt ggtgcacgtg aaaaaaatct gctgatgaat ctggtttggg
     5881 atgttgcagc aagcgaacat agcacccgtg cactggtttt tgaagaacag catgcactga
     5941 gcgaaccgct gctgcgcgat aatctggttc tggattatga ttatcgtgaa agcaccagcc
     6001 tgattcgtcg tctggttggt ctgaatgcaa aatgataata ctagagccag gcatcaaata
     6061 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     6121 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt tt
//
