LOCUS       sequence-33143-e         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Meierhofer D, Wang X, Huang L, Kaiser P
  TITLE     Quantitative analysis of global ubiquitination in HeLa cells by mass
            spectrometry.
  JOURNAL   J Proteome Res. 2008 Oct . 7(10):4566-76.
  PUBMED    18781797
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Proteome 
            Res. 2008 Oct . 7(10):4566-76."
COMMENT     Sequence Label: pQCXIP HBT-Ubiquitin
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             140..157
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             410..430
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             476..700
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="QIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFA
                     GKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 gacctccata gaaganaccg ggaccgatcc agcctccgcg gccgggaacg gtgcannnnn
       61 nnggacctgc aggaattgat ccgcggccgc accggtaggc ctcgtacgct taattaacgg
      121 atccattatg aggggttcac atcatcacca ccatcatgct ggaaaggccg gtgaaggtga
      181 aatccctgcc cctcttgctg gtaccgtttc taagatactg gtaaaagaag gtgacactgt
      241 taaagctggt caaacagttc tggtgctgga ggctatgaaa atggagacag aaattaacgc
      301 tcctactgac ggaaaagttg aaaaggtgtt agttaaggaa agagatgctg ttcaaggtgg
      361 tcaaggtcta atcaagatcg gcgactacga tatacccaca accgccagcg agaatctgta
      421 ctttcaatca gagttaaaaa ccgccgcact agctcaacat gacgaggctt ctagacagat
      481 tttcgtcaag actttgaccg gtaaaaccat aacattggaa gttgaatctt ccgataccat
      541 cgacaacgtt aagtcgaaaa ttcaagacaa ggaaggtatc cctccagatc aacaaagatt
      601 gatctttgcc ggtaagcagc tagaagacgg tagaacgctg tctgattaca acattcagaa
      661 ggagtccacc ttacatcttg tcttaagact aagaggtggt tgaggatccg gaattccgcc
      721 cc
//
