LOCUS       sequence-33159-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral expression of eNpHR 3.0 fused to EYFP driven by 
            human Synapsin I promoter for optogenetic inhibition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester 
            I, Goshen I, Thompson KR, Deisseroth K
  TITLE     Molecular and cellular approaches for diversifying and extending 
            optogenetics.
  JOURNAL   Cell. 2010 Apr 2. 141(1):154-65.
  PUBMED    20303157
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Apr 2. 141(1):154-65."
COMMENT     Sequence Label: pLenti-hSyn-eNpHR 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        31..478
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
ORIGIN
        1 gtgtctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga ccaggatgag
       61 gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc
      121 cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg
      181 cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc
      241 gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac
      301 tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca
      361 cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc
      421 agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc
      481 gagaaaccgg taggccacca tgacagagac cctgcctccc gtgaccgaga gtgccgtggc
      541 ccttcaagcc gaggttaccc aaagggagtt gttcgagttc gtgctgaacg accctttgct
      601 tgcaagcagt ctctatatca acatcgcact tgcaggactg agtatactgc tgttcgtttt
      661 tatgacccga ggactcgatg atccacgggc aaaacttatt gctgtgtcaa ccatccttgt
      721 gcctgtcgtc agcattgcct cctacactgg attggcgagc ggcctgacaa tttccgttct
      781 tgaaatgcca gcgggccatt ttgcagaagg cagctcagtg atgctgggag gagaagaggt
      841 agatggtgta gtcaccatgt ggggacggta tctcacctgg gcactttcca cgcccatgat
      901 tctcctcgct ctgggtctcc tggccggaag caatgctaca aagctcttca cagctatcac
//
