LOCUS       sequence-331641-        6331 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mRNA of NCX2 (Rat brain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Li Z, Matsuoka S, Hryshko LV, Nicoll DA, Bersohn MM, Burke EP, 
            Lifton RP, Philipson KD
  TITLE     Cloning of the NCX2 isoform of the plasma membrane Na(+)-Ca2+ 
            exchanger.
  JOURNAL   J Biol Chem. 1994 Jul 1;269(26):17434-9.
  PUBMED    8021246
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1994-07-1"; volume: "269(26)"; pages: "17434-9"
COMMENT     Sequence Label: pNCX2
FEATURES             Location/Qualifiers
     source          1..6331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    21..42
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(57..62,63..80,81..87)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    95..111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     100..122
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     119..135
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     119..135
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        156..174
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3634..3650)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3635..3651)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3675..3694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3676..3694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3701..3718)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3701..3717)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3710..3732)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3859..4314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3946..3965)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4156..4177
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4340..4444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4445..4513,4514..5305)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4663..4682)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5476..6064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5965..5984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6218..6235
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
       61 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      121 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
      181 aagctggagc tccccccatg gctcccttgg ctttggtagg ggttgccctc cttctggggg
      241 caccccactg cttgggggaa gctactccga ccccatccct gccacctccc ccagctaatg
      301 acagcgatgc cagcccaggc ggctgccaag gttcctaccg ctgccaacca ggggtgctgc
      361 tgcctgtgtg ggaacccgac gacccatcac tgggggacaa ggctgcacgg gccgtggtgt
      421 actttgtggc catggtctac atgttcttgg gtgtgtctat cattgctgat cgttttatgg
      481 catccattga ggtcatcaca tccaaggaga aagagatcac catcaccaag gcaaatgggg
      541 agaccagcgt gggcactgta cgcatctgga atgaaacggt gtccaacctt acactcatgg
      601 ccctgggctc ctcagcacct gagattctgc tgtctgtcat cgaggtctgt ggccacaact
      661 tccaggcggg tgagctaggc ccaggcacca tcgtgggcag tgccgccttc aacatgtttg
      721 tggtcattgc tgtgtgtgtg tatgtcatcc cggctggtga gagccgtaag atcaagcact
      781 taagggtctt cttcgtcaca gcctcttgga gcatctttgc ctatgtctgg ctttatctca
      841 ttctagcagt tttctctcca ggcgtggtcc aggtgtggga ggcactgctc acgctggtct
      901 tcttcccggt gtgtgtggtg ttcgcctgga tggcggacaa gcgactgctc ttctacaagt
      961 acgtgtacaa gcgctatcgc accgaccctc gcagcggaat catcatcggg gcagagggag
     1021 acccgcccaa gagcatcgag ctggatggca cattcgtggg cactgaggtc ccaggcgagc
     1081 tgggtgcatt gggcacaggt cccgctgagg cgcgtgagct ggacgccagc cggcgcgagg
     1141 tcatccagat tcttaaggac ttaaagcaga agcacccgga taaggacctg gagcagctgg
     1201 tgggcatcgc caagtactat gcactgctgc accagcagaa gagccgtgcc ttctaccgca
     1261 tccaggccac gcggctgatg acaggtgcgg gcaacgtgct gcgccgacac gctgcggatg
     1321 ctgcccgcag gcctggggcc aacgatggtg cccccgatga tgaggacgat ggtgccagcc
     1381 gcatcttctt tgagcccagc ctctaccact gcctggagaa ctgcgggtca gtgctgctgt
     1441 cggtggcttg ccagggtggt gagggcaaca gcaccttcta cgtggattac cgcacggagg
     1501 atggctctgc aaaggcaggc tccgattatg agtacagcga gggcacgctg gtgttcaagc
     1561 ccggggagac gcagaaggag ctgcgcatcg gcatcatcga cgacgacatc ttcgaggagg
     1621 acgagcactt cttcgtgagg ctgctgaacc tgcgtgtggg cgatgctcag ggcatgttcg
     1681 agcccgacgg cggtgggcgg cccaaggggc ggctggtggc gccactgctg gccaccgtca
     1741 ccattctgga cgacgaccac gcgggcatct tctccttcca ggaccgcctg ctgcatgtga
     1801 gcgagtgcat gggcaccgtg gatgtgcgtg tggtgcgcag ctctggcgca cgtggcaccg
     1861 tacgccttcc ctaccgcaca gtggacggca cggctcgtgg tggtggtgtg cactacgagg
     1921 atgcttgtgg agagctggag ttcggcgatg acgagaccat gaagactctt caggtcaaga
     1981 tagtggacga cgaagagtat gagaagaagg acaacttctt catcgagctg ggccagcccc
     2041 agtggcttaa gcggggcatc tcagctctgc tactcaacca aggggatgga gacaggaagc
     2101 tgactgcaga ggaggaggag gcccagagga tagcagagat gggcaagcca gttcttgggg
     2161 agaactgtcg cctcgaggtc atcatcgagg agtcttatga ctttaagaat acggtggata
     2221 aactcataaa gaaaacaaac ctggccttgg tgattgggac ccactcatgg agggagcagt
     2281 ttttagaggc agttacagtg agcgcagggg acgaggagga ggatgaggat gggtctcgtg
     2341 aggagcggct gccatcctgc tttgactacg tgatgcactt cctgacggtg ttctggaaag
     2401 ttctgttcgc ctgccttcca cccacggagt actgccatgg ctgggcctgc tttggtgtct
     2461 gcatcctggt cattggtctg ctcactgccc tcatcggaga cctggcctca cactttgggt
     2521 gcaccgtggg cctcaaggac tcagtcaacg ccgtggtctt cgtggctctg ggcacctcca
     2581 tccctgacac gtttgccagc aaggtggccg cgctgcagga ccagtgcgcc gacgcgtcca
     2641 tcggtaacgt gaccggctcc aatgcggtga acgtgttcct gggcctgggt gtggcctggt
     2701 cggtggccgc agtgtactgg gcggtgcagg gtcgcccctt cgaggtgcgt acaggcacgc
     2761 tggccttctc ggtcacactg ttcaccgtct tcgccttcgt gggcatcgca gtgctcttgt
     2821 accggcgccg gccacacatc ggcggcgagc tgggcggccc gcggggaccc aagctagcca
     2881 ccaccgctct cttcctgggc ctctggttcc tctacattct cttcgccagc ctggaggctt
     2941 attgccacat tcggggcttc tagggcccca gccgctaccc tgagggtggg gacttgttgg
     3001 cacttgctct tggacccacc cctgtctccc tacccagact caacctgggt acttcctagg
     3061 ctcctcctca tcagccttct tcctgggatc agcctccctt tccccctgga atcccatccc
     3121 ttgccctccc ttctcagagg cacccccttc agcccatctt tgtgagttac acctgagccc
     3181 tctggccaga cacccagccc cgaattcgcg tactttgcct gagtcccggc cgctgctgcc
     3241 actcggcaag gctggcgtgt gtactctctg cctgcctttc atcccatgct gtggtggcga
     3301 aggggaatca atcccttgca aagtttgccc catttggata aatgtctata tgtaaattac
     3361 aggctggttg gaggaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3481 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3541 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3601 aaaaaaaaaa aaggaattcg atatcaagct tatcgatacc gtcgacctcg agggggggcc
     3661 cggtacccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     3721 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     3781 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     3841 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     3901 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     3961 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4021 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4081 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4141 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4201 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4261 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     4321 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4381 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4441 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4501 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4561 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4621 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4681 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4741 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4801 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4861 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4921 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4981 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5041 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5101 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5161 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5221 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5281 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5341 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5401 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5461 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5521 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5581 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     5641 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5701 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5761 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5821 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5881 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5941 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6001 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6061 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6121 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6181 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6241 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6301 ctggcacgac aggtttcccg actggaaagc g
//
