LOCUS       sequence-331874-        5634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replaces Addgene plasmid 118461. Yeast tagging vector to create a 
            gene fusion with yeast optimized tdTomato with CaUra3 selection; 
            Contains the linker region at the 5' end of the FP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
  TITLE     In vivo characterisation of fluorescent proteins in budding yeast.
  JOURNAL   Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z.
  PUBMED    30783202
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-19"; volume: "9(1):2234. doi"; pages: " 
            10.1038/s41598-019-38913-z"
COMMENT     Sequence Label: pFA6a-link-ytdTomato-URA3
FEATURES             Location/Qualifiers
     source          1..5634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..344
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      1163..1360
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1467..1486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1468..1486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1573..1590)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1744..2332)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1824..1843)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2503..3294,3295..3363))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3126..3145
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3364..3468)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3536..3554
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3592..3614)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3714..3733
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3814..3832
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3814..3831
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     terminator      5373..5560
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5428..5452)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
       61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
      121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag
      181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
      241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
      301 ttgctaggat acagttctca catcacatcc gaacataaac aaccatgaca gtcaacacta
      361 agacctatag tgagagagca gaaactcatg cctcaccagt agcacaacga ttatttcgat
      421 taatggaact gaagaaaacc aatttatgtg catcaattga tgttgatacc actaaggaat
      481 tccttgaatt aattgataaa ttgggtcctt atgtatgctt aatcaagact catattgata
      541 taatcaatga tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac
      601 atcaatttat gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtgaagaaac
      661 aatatattgg tggagtttat aaaattagta gttgggcaga tattactaat gctcatggtg
      721 tcactgggaa tggagtagtt gaaggattaa aacagggagc taaagaaacc accaccaacc
      781 aagagccaag agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg
      841 gagaatattc tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat
      901 ttattgccca acgtgatatg ggtggacaag aagaaggatt tgattggctt attatgacac
      961 ctggagttgg attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg
     1021 aagttgttag cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa
     1081 gagatccaga tattgaaggt aaaaggtata gagatgctgg ttggaatgct tatttgaaaa
     1141 agactggcca attataatca gtactgacaa taaaaagatt cttgttttca agaacttgtc
     1201 atttgtatag tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta
     1261 tatttttttt cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat
     1321 atcatgcgtc aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg
     1381 ccgccatcca gtgtcgaaaa cgagctcgaa ttcatcgatg atatcagatc cactagtggc
     1441 ctatgcggcc gcggatctgc cggtctccct atagtgagtc gtattaattt cgataagcca
     1501 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1561 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1621 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1681 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1741 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1801 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1861 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1921 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1981 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2041 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2101 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2161 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2221 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2281 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2341 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2401 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2461 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2521 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2581 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2641 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2701 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2761 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2821 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2881 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2941 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3001 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3061 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3121 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3181 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3241 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3301 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3361 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3421 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3481 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     3541 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     3601 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     3661 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
     3721 agagcagatt gtactgagag tgcaccatat ggacatattg tcgttagaac gcggctacaa
     3781 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaacgcggcc
     3841 gccagctgaa gcttcgtacg ctgcaggtcg acggatcggt gacggtgctg gtttaattaa
     3901 catggttagt aaaggtgagg aagtgattaa ggaattcatg aggtttaaag ttagaatgga
     3961 aggttctatg aatggtcatg aatttgaaat tgaaggtgaa ggtgaaggta gaccatatga
     4021 aggtacacaa actgctaaat tgaaagttac aaaaggtggt ccattaccat ttgcatggga
     4081 tattttatct ccacagttta tgtatggtag taaagcatat gttaaacatc cagctgatat
     4141 tccagattat aagaaattaa gttttcctga aggttttaaa tgggaaagag taatgaattt
     4201 tgaagatggt ggtttagtaa cagttactca agattcttct ttacaagatg gtacattaat
     4261 atataaagtt aaaatgagag gtactaattt tccacctgat ggtccagtta tgcagaagaa
     4321 aacaatgggt tgggaagcct caactgaaag attgtaccct agagatggag ttttgaaagg
     4381 tgaaattcat caagctctta agttaaaaga tggaggtcac tatttggttg agtttaaaac
     4441 tatatacatg gctaaaaaac ctgttcaatt gccaggttat tattatgttg atactaaatt
     4501 agatattact tcacataatg aagattatac tattgttgaa caatatgaaa gatctgaagg
     4561 tagacatcat ttgtttttgg gtcatggtac tggtagtact ggttctggtt cttcaggtac
     4621 tgcttctagt gaagataata atatggctgt aatcaaagag ttcatgaggt ttaaagttag
     4681 aatggaaggt tctatgaatg gtcatgagtt tgaaatcgaa ggtgaaggag aaggtagacc
     4741 atatgaaggt actcaaactg ctaaattgaa agttactaaa ggtggtccat tgccatttgc
     4801 ttgggatata ttgtcacctc agtttatgta tggttctaaa gcatatgtta aacatccagc
     4861 tgatattcct gattataaaa aattatcatt tcctgaaggt tttaaatggg aaagagttat
     4921 gaattttgaa gatggtggtt tagttactgt aactcaagat tcttcattgc aagatggtac
     4981 attaatatat aaagttaaaa tgagaggtac taattttcca cctgatggtc cagttatgca
     5041 gaaaaagact atgggttggg aagcatctac tgaaagatta tatcctagag atggtgtttt
     5101 aaaaggtgaa attcaccaag ccttaaaatt gaaagatggt ggtcattatt tggttgaatt
     5161 caaaacaatc tacatggcta aaaaaccagt tcagctgccc ggatactatt atgtcgatac
     5221 taaactggat attacttcac acaatgaaga ctacacaatt gttgaacagt atgaaagatc
     5281 agagggtaga catcacttgt ttttatacgg tatggatgaa ttatataaat aagcggccgc
     5341 ctcgaggtcg acggcgcgcc acttctaaat aagcgaattt cttatgattt atgattttta
     5401 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     5461 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     5521 tatagtatga ggtcgctctt attgaccaca cctctaccgg cagatccgct agggataaca
     5581 gggtaatata gatctgttta gcttgcctcg tccccgccgg gtcacccggc cagc
//
