LOCUS       sequence-332203-       10926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to NLS, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN074
FEATURES             Location/Qualifiers
     source          1..10926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        175..588
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             4298..4318
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    4667..4700
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4746..6102
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4746..5089
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5090..5899
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5157..5176)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      5905..6102
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6150..6183
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6251..6271)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6251..6269)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6290..6306)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6290..6306)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6303..6325)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6314..6330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6338..6344,6345..6362,6363..6368))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6383..6404
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6521..6538)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6692..7280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6772..6791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7451..8242,8243..8311))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8074..8093
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8312..8416)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8453..8956
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8997..9015
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9053..9075)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9175..9194
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9215..9495
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9496..10170
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10273..10728)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10410..10431)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10622..10641
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10854..10876
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10868..10885
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10869..10885
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10895..10914
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10895..10913
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cttggctgat aatagcgtat aaacaatgca tactttgtac gttcaaaata caatgcagta
       61 gatatattta tgcatattac atataataca tatcacatag gaagcaacag gcgcgttgga
      121 cttttaattt tcgaggaccg cgaatcctta catcacaccc aatcccccac aagtgatccc
      181 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
      241 ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa tttcccctct
      301 ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga aaaaagacac
      361 cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt
      421 cttgaaaatt tttttttttg atttttttct ctttcgatga cctcccattg atatttaagt
      481 taataaacgg tcttcaattt ctcaagtttc agtttcattt ttcttgttct attacaactt
      541 tttttacttc ttgctcatta gaaagaaagc atagcaatct aatctaagtt ttaattacaa
      601 aatgtctaag ttggaaaaat tcaccaactg ttactctttg tctaagactt tgagattcaa
      661 ggccatccca gttggtaaga cccaagaaaa catcgacaac aagagactat tagttgaaga
      721 tgaaaagaga gctgaagact acaagggtgt caagaaattg ttggacagat actacttgtc
      781 ttttatcaac gacgttttgc attccatcaa gctaaagaac ttgaataact acatctcttt
      841 gttcagaaag aagactagaa ctgaaaagga aaataaggaa ttggaaaact tggaaatcaa
      901 cttgagaaag gaaattgcta aggctttcaa gggtaatgaa ggttacaagt ctttattcaa
      961 gaaagacatc attgaaacca ttttgccaga atttttggat gataaggatg aaattgcttt
     1021 ggttaactct ttcaacggtt tcaccactgc tttcactggt ttcttcgaca acagagaaaa
     1081 catgttctcc gaggaagcta aatccacttc tattgctttc agatgtatca acgaaaactt
     1141 gacccgttac atctctaaca tggacatttt tgaaaaggtc gacgccatct ttgacaagca
     1201 cgaagtccaa gaaatcaagg aaaagatctt aaactccgac tacgatgtcg aagatttctt
     1261 cgaaggtgaa ttcttcaact ttgttttaac ccaagaaggt atcgatgtct acaacgccat
     1321 tatcggtggt tttgtcactg aatctggtga aaagatcaag ggtttgaacg aatacattaa
     1381 cttgtacaac caaaagacca aacaaaaatt gccaaagttc aagccattgt acaagcaagt
     1441 tttgtctgac agagaatctt tgtcttttta cggtgaaggg tacacctctg acgaagaagt
     1501 cttggaagtc ttcagaaaca ctttgaacaa gaactctgaa atcttctcct ccatcaagaa
     1561 gttagaaaag ttgttcaaga acttcgatga atactcttct gctggtatct tcgttaagaa
     1621 cggtccagcc atctctacca tttctaagga tatctttggt gaatggaacg tcattagaga
     1681 caaatggaac gctgaatacg atgacatcca tttgaagaaa aaggctgttg tcaccgaaaa
     1741 gtacgaagac gacagaagaa aatccttcaa gaagatcggt tccttctcct tggaacaatt
     1801 acaagaatac gccgatgccg atttgtccgt tgtcgaaaaa ttgaaggaaa ttattattca
     1861 aaaggttgat gaaatttaca aagtttacgg ttcctctgaa aagttattcg atgctgattt
     1921 cgtcttggaa aagtctttga agaagaacga cgctgttgtc gctatcatga aggacttgtt
     1981 ggactctgtc aaatctttcg aaaactatat caaggccttc ttcggtgaag gtaaggaaac
     2041 taacagagat gaatccttct acggtgactt tgtcttggct tacgatattt tgttgaaggt
     2101 tgaccacatc tacgatgcca tcagaaacta cgttactcaa aagccatact ctaaggacaa
     2161 attcaagttg tacttccaaa acccacaatt catgggtggt tgggataagg acaaggaaac
     2221 tgactacaga gctaccattt tgagatacgg ttccaagtac tacttggcca tcatggacaa
     2281 gaagtacgcc aagtgtttgc aaaagattga caaggacgat gtcaacggta actacgaaaa
     2341 gattaactac aagttgttgc caggtccaaa caagatgttg ccaaaggttt tcttctccaa
     2401 aaagtggatg gcttactaca acccatctga agacatccaa aagatctaca agaacggtac
     2461 tttcaaaaag ggtgacatgt tcaacttaaa cgactgtcac aagttgatcg acttcttcaa
     2521 ggactccatc tctagatacc caaaatggtc caacgcttac gatttcaact tctctgaaac
     2581 tgaaaaatac aaggatattg ctggtttcta ccgtgaagtc gaggaacaag gttataaggt
     2641 ttctttcgaa tccgcttcta agaaagaagt tgacaaatta gtcgaagaag gtaagttgta
     2701 catgttccaa atctacaaca aagatttctc cgacaagtct cacggtactc caaacttgca
     2761 caccatgtac ttcaagttgc tattcgatga aaacaaccac ggtcaaatca gattgtctgg
     2821 tggtgctgaa ttgttcatga gacgtgcttc tctaaagaag gaagaattag tcgtccaccc
     2881 agctaactct ccaattgcca acaagaaccc agacaaccct aagaagacca ccactttgtc
     2941 ctacgacgtt tacaaggaca agagattctc cgaagaccaa tacgaattgc acattccaat
     3001 tgctatcaac aagtgtccaa agaacatctt caagatcaac actgaagtca gagttttgtt
     3061 aaagcacgat gacaaccctt acgttattgg tatcgaccgt ggtgaaagaa atttgttgta
     3121 cattgttgtt gttgacggta agggtaacat cgttgaacaa tactccttga acgaaatcat
     3181 caacaacttc aacggtatta gaatcaagac tgattaccac tctttgttgg ataagaagga
     3241 aaaggaacgt tttgaagctc gtcaaaactg gacctctatt gaaaacatca aagaattgaa
     3301 ggctggttac atcagtcaag ttgtccacaa gatctgtgaa ttggtcgaga agtacgatgc
     3361 cgttattgcc ttggcagatt tgaactctgg ttttaagaac tctcgtgtca aggttgaaaa
     3421 gcaagtctac caaaagttcg aaaagatgtt aatcgacaaa ttgaactaca tggttgacaa
     3481 gaaatccaac ccatgtgcta ccggtggtgc tttgaaaggt taccaaatca ccaacaaatt
     3541 cgaatctttc aaatctatgt ccactcaaaa cgggttcatc ttctacattc cagcttggtt
     3601 gacctccaag atcgacccat ctaccggttt cgttaacttg ttgaagacca agtacacttc
     3661 cattgctgat tccaagaagt tcatctcttc tttcgacaga atcatgtacg ttccagaaga
     3721 agacttgttc gaattcgcct tggactataa gaacttctcc agaaccgatg ctgactacat
     3781 taagaaatgg aaattgtact cctacggtaa cagaatcaga attttcagaa acccaaagaa
     3841 aaacaacgtt ttcgattggg aagaagtttg tttgacttct gcctacaagg aattattcaa
     3901 caaatacggt atcaactacc aacaaggtga tatcagagct ttgttgtgtg aacaatctga
     3961 caaggctttc tactcttcct tcatggcttt gatgtccttg atgttgcaaa tgagaaactc
     4021 catcactggt agaactgatg tcgacttcct catttctcca gttaagaatt ctgacggtat
     4081 tttctacgac tctagaaatt acgaagctca agaaaacgct attttgccaa agaacgctga
     4141 tgctaacggt gcttacaata ttgctagaaa ggttttgtgg gctatcggtc aattcaagaa
     4201 ggctgaagac gaaaagctag acaaggtcaa gattgctatt tctaacaagg aatggttgga
     4261 atacgctcaa acctccgtca agcactccag agctgatcca aagaagaaga gaaaggtata
     4321 aaggagttaa aggcaaagtt ttcttttcta gagccgttcc cacaaataat tatacgtata
     4381 tgcttctttt cgtttactat atatctatat ttacaagcct ttattcactg atgcaatttg
     4441 tttccaaata cttttttgga gatctcataa ctagatatca tgatggcgca acttggcgct
     4501 atcttaatta ctctggctgc caggcccgtg tagagggccg caagaccttc tgtacgccat
     4561 atagtctcta agaacttgaa caagtttcta gacctattgc cgcctttcgg atcgctattg
     4621 ttgcggccgc cagctgaagc ttcgtacgct gcaggtcgac gaattctacc gttcgtataa
     4681 tgtatgctat acgaagttat agatctgttt agcttgcctc gtccccgccg ggtcacccgg
     4741 ccagcgacat ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg
     4801 catgatgtga ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat
     4861 ccatacattt tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctgcagacct
     4921 gcgagcaggg aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt
     4981 agagaaatat aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta
     5041 aaatcttgct aggatacagt tctcacatca catccgaaca taaacaacca tgggtaagga
     5101 aaagactcac gtttcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     5161 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa
     5221 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     5281 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     5341 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gcaaaacagc
     5401 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     5461 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     5521 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     5581 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataagctttt
     5641 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     5701 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     5761 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     5821 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     5881 gctcgatgag tttttctaat cagtactgac aataaaaaga ttcttgtttt caagaacttg
     5941 tcatttgtat agttttttta tattgtagtt gttctatttt aatcaaatgt tagcgtgatt
     6001 tatatttttt ttcgcctcga catcatctgc ccagatgcga agttaagtgc gcagaaagta
     6061 atatcatgcg tcaatcgtat gtgaatgctg gtcgctatac tgctgtcgat tcgatactaa
     6121 cgccgccatc cagtgtcgaa aacgagctca taacttcgta taatgtatgc tatacgaacg
     6181 gtagaattcg atatcagatc cactagtggc ctatgcggcc gccaccgcgg tggagctcca
     6241 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     6301 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6361 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6421 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6481 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6541 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6601 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7321 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7381 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7441 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7501 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7561 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7621 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7681 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7741 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7801 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7861 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7921 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7981 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8041 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8101 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8161 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8221 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8281 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8341 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8401 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg
     8461 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     8521 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     8581 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     8641 aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt
     8701 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     8761 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     8821 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     8881 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     8941 ttacaggcaa gcgatccgtc taagaaacca ttattatcat gacattaacc tataaaaata
     9001 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     9061 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     9121 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     9181 cagagcagat tgtactgaga gtgcaccata gatcacgaca ttactatata tataatatag
     9241 gaagcattta atagaacagc atcgtaatat atgtgtactt tgcagttatg acgccagatg
     9301 gcagtagtgg aagatattct ttattgaaaa atagcttgtc accttacgta caatcttgat
     9361 ccggagcttt tctttttttg ccgattaaga attaattcgg tcgaaaaaag aaaaggagag
     9421 ggccaagagg gagggcattg gtgactattg agcacgtgag tatacgtgat taagcacaca
     9481 aaggcagctt ggagtatgtc tgttattaat ttcacaggta gttctggtcc attggtgaaa
     9541 gtttgcggct tgcagagcac agaggccgca gaatgtgctc tagattccga tgctgacttg
     9601 ctgggtatta tatgtgtgcc caatagaaag agaacaattg acccggttat tgcaaggaaa
     9661 atttcaagtc ttgtaaaagc atataaaaat agttcaggca ctccgaaata cttggttggc
     9721 gtgtttcgta atcaacctaa ggaggatgtt ttggctctgg tcaatgatta cggcattgat
     9781 atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct cggtttgcca
     9841 gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc agcttcacag
     9901 aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg tgaacttttg
     9961 gattggaact cgatttctga ctgggttgga aggcaagaga gccccgaaag cttacatttt
    10021 atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt aaatggcgtt
    10081 attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc taacaaaata
    10141 gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt atttaagtat
    10201 tgtttgtgca cttgccgatc tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10261 taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
    10321 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
    10381 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
    10441 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
    10501 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
    10561 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
    10621 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
    10681 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt
    10741 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
    10801 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
    10861 acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt
    10921 gggtac
//
