LOCUS       sequence-332209-       11050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A 
            fused to Mxi1, codon optimized for Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11050)
  AUTHORS   Ciurkot K, Gorochowski TE, Roubos JA, Verwaal R
  TITLE     Efficient multiplexed gene regulation in Saccharomyces cerevisiae 
            using dCas12a.
  JOURNAL   Nucleic Acids Res. 2021 Jul 1. pii: 6312738. doi: 
            10.1093/nar/gkab529.
  PUBMED    34197613
REFERENCE   2  (bases 1 to 11050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2021 Jul 1. pii: 6312738. doi: 10.1093/nar/gkab529."
COMMENT     Sequence Label: pCSN069
FEATURES             Location/Qualifiers
     source          1..11050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        207..621
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     protein_bind    4823..4856
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4902..6258
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        4902..5245
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             5246..6055
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5313..5332)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      6061..6258
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6306..6339
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6407..6427)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6407..6425)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6446..6462)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6446..6462)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6459..6481)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6470..6486
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6494..6500,6501..6518,6519..6524))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6539..6560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6677..6694)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6848..7436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6928..6947)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7607..8398,8399..8467))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8230..8249
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8468..8572)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    8609..9112
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9153..9171
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9209..9231)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9331..9350
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9371..9651
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9652..10326
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10429..10884)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10566..10587)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10778..10797
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11010..11032
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11024..11041
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11025..11041
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattgggt accttggctg ataatagcgt ataaacaatg
       61 catactttgt acgttcaaaa tacaatgcag tagatatatt tatgcatatt acatataata
      121 catatcacat aggaagcaac aggcgcgttg gacttttaat tttcgaggac cgcgaatcct
      181 tacatcacac ccaatccccc acaagtgatc ccccacacac catagcttca aaatgtttct
      241 actccttttt tactcttcca gattttctcg gactccgcgc atcgccgtac cacttcaaaa
      301 cacccaagca cagcatacta aatttcccct ctttcttcct ctagggtgtc gttaattacc
      361 cgtactaaag gtttggaaaa gaaaaaagac accgcctcgt ttctttttct tcgtcgaaaa
      421 aggcaataaa aatttttatc acgtttcttt ttcttgaaaa tttttttttt ttgatttttt
      481 tctctttcga tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt
      541 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
      601 agcatagcaa tctaatctaa gttttaatta caaaatgtct aagttggaaa aattcaccaa
      661 ctgttactct ttgtctaaga ctttgagatt caaggccatc ccagttggta agacccaaga
      721 aaacatcgac aacaagagac tattagttga agatgaaaag agagctgaag actacaaggg
      781 tgtcaagaaa ttgttggaca gatactactt gtcttttatc aacgacgttt tgcattccat
      841 caagctaaag aacttgaata actacatctc tttgttcaga aagaagacta gaactgaaaa
      901 ggaaaataag gaattggaaa acttggaaat caacttgaga aaggaaattg ctaaggcttt
      961 caagggtaat gaaggttaca agtctttatt caagaaagac atcattgaaa ccattttgcc
     1021 agaatttttg gatgataagg atgaaattgc tttggttaac tctttcaacg gtttcaccac
     1081 tgctttcact ggtttcttcg acaacagaga aaacatgttc tccgaggaag ctaaatccac
     1141 ttctattgct ttcagatgta tcaacgaaaa cttgacccgt tacatctcta acatggacat
     1201 ttttgaaaag gtcgacgcca tctttgacaa gcacgaagtc caagaaatca aggaaaagat
     1261 cttaaactcc gactacgatg tcgaagattt cttcgaaggt gaattcttca actttgtttt
     1321 aacccaagaa ggtatcgatg tctacaacgc cattatcggt ggttttgtca ctgaatctgg
     1381 tgaaaagatc aagggtttga acgaatacat taacttgtac aaccaaaaga ccaaacaaaa
     1441 attgccaaag ttcaagccat tgtacaagca agttttgtct gacagagaat ctttgtcttt
     1501 ttacggtgaa gggtacacct ctgacgaaga agtcttggaa gtcttcagaa acactttgaa
     1561 caagaactct gaaatcttct cctccatcaa gaagttagaa aagttgttca agaacttcga
     1621 tgaatactct tctgctggta tcttcgttaa gaacggtcca gccatctcta ccatttctaa
     1681 ggatatcttt ggtgaatgga acgtcattag agacaaatgg aacgctgaat acgatgacat
     1741 ccatttgaag aaaaaggctg ttgtcaccga aaagtacgaa gacgacagaa gaaaatcctt
     1801 caagaagatc ggttccttct ccttggaaca attacaagaa tacgccgatg ccgatttgtc
     1861 cgttgtcgaa aaattgaagg aaattattat tcaaaaggtt gatgaaattt acaaagttta
     1921 cggttcctct gaaaagttat tcgatgctga tttcgtcttg gaaaagtctt tgaagaagaa
     1981 cgacgctgtt gtcgctatca tgaaggactt gttggactct gtcaaatctt tcgaaaacta
     2041 tatcaaggcc ttcttcggtg aaggtaagga aactaacaga gatgaatcct tctacggtga
     2101 ctttgtcttg gcttacgata ttttgttgaa ggttgaccac atctacgatg ccatcagaaa
     2161 ctacgttact caaaagccat actctaagga caaattcaag ttgtacttcc aaaacccaca
     2221 attcatgggt ggttgggata aggacaagga aactgactac agagctacca ttttgagata
     2281 cggttccaag tactacttgg ccatcatgga caagaagtac gccaagtgtt tgcaaaagat
     2341 tgacaaggac gatgtcaacg gtaactacga aaagattaac tacaagttgt tgccaggtcc
     2401 aaacaagatg ttgccaaagg ttttcttctc caaaaagtgg atggcttact acaacccatc
     2461 tgaagacatc caaaagatct acaagaacgg tactttcaaa aagggtgaca tgttcaactt
     2521 aaacgactgt cacaagttga tcgacttctt caaggactcc atctctagat acccaaaatg
     2581 gtccaacgct tacgatttca acttctctga aactgaaaaa tacaaggata ttgctggttt
     2641 ctaccgtgaa gtcgaggaac aaggttataa ggtttctttc gaatccgctt ctaagaaaga
     2701 agttgacaaa ttagtcgaag aaggtaagtt gtacatgttc caaatctaca acaaagattt
     2761 ctccgacaag tctcacggta ctccaaactt gcacaccatg tacttcaagt tgctattcga
     2821 tgaaaacaac cacggtcaaa tcagattgtc tggtggtgct gaattgttca tgagacgtgc
     2881 ttctctaaag aaggaagaat tagtcgtcca cccagctaac tctccaattg ccaacaagaa
     2941 cccagacaac cctaagaaga ccaccacttt gtcctacgac gtttacaagg acaagagatt
     3001 ctccgaagac caatacgaat tgcacattcc aattgctatc aacaagtgtc caaagaacat
     3061 cttcaagatc aacactgaag tcagagtttt gttaaagcac gatgacaacc cttacgttat
     3121 tggtatcgac cgtggtgaaa gaaatttgtt gtacattgtt gttgttgacg gtaagggtaa
     3181 catcgttgaa caatactcct tgaacgaaat catcaacaac ttcaacggta ttagaatcaa
     3241 gactgattac cactctttgt tggataagaa ggaaaaggaa cgttttgaag ctcgtcaaaa
     3301 ctggacctct attgaaaaca tcaaagaatt gaaggctggt tacatcagtc aagttgtcca
     3361 caagatctgt gaattggtcg agaagtacga tgccgttatt gccttggcag atttgaactc
     3421 tggttttaag aactctcgtg tcaaggttga aaagcaagtc taccaaaagt tcgaaaagat
     3481 gttaatcgac aaattgaact acatggttga caagaaatcc aacccatgtg ctaccggtgg
     3541 tgctttgaaa ggttaccaaa tcaccaacaa attcgaatct ttcaaatcta tgtccactca
     3601 aaacgggttc atcttctaca ttccagcttg gttgacctcc aagatcgacc catctaccgg
     3661 tttcgttaac ttgttgaaga ccaagtacac ttccattgct gattccaaga agttcatctc
     3721 ttctttcgac agaatcatgt acgttccaga agaagacttg ttcgaattcg ccttggacta
     3781 taagaacttc tccagaaccg atgctgacta cattaagaaa tggaaattgt actcctacgg
     3841 taacagaatc agaattttca gaaacccaaa gaaaaacaac gttttcgatt gggaagaagt
     3901 ttgtttgact tctgcctaca aggaattatt caacaaatac ggtatcaact accaacaagg
     3961 tgatatcaga gctttgttgt gtgaacaatc tgacaaggct ttctactctt ccttcatggc
     4021 tttgatgtcc ttgatgttgc aaatgagaaa ctccatcact ggtagaactg atgtcgactt
     4081 cctcatttct ccagttaaga attctgacgg tattttctac gactctagaa attacgaagc
     4141 tcaagaaaac gctattttgc caaagaacgc tgatgctaac ggtgcttaca atattgctag
     4201 aaaggttttg tgggctatcg gtcaattcaa gaaggctgaa gacgaaaagc tagacaaggt
     4261 caagattgct atttctaaca aggaatggtt ggaatacgct caaacctccg tcaagcacgg
     4321 ttcttccaag ttgggtggtt ctggtggttc catggaacgt gtcagaatga tcaacgttca
     4381 aagattgttg gaagctgctg aattcttgga aagaagagaa agagaatgtg aacacggtta
     4441 cgcctcctct ttcccatcca tgccatctcc aagataaagg agttaaaggc aaagttttct
     4501 tttctagagc cgttcccaca aataattata cgtatatgct tcttttcgtt tactatatat
     4561 ctatatttac aagcctttat tcactgatgc aatttgtttc caaatacttt tttggagatc
     4621 tcataactag atatcatgat ggcgcaactt ggcgctatct taattactct ggctgccagg
     4681 cccgtgtaga gggccgcaag accttctgta cgccatatag tctctaagaa cttgaacaag
     4741 tttctagacc tattgccgcc tttcggatcg ctattgttgc ggccgccagc tgaagcttcg
     4801 tacgctgcag gtcgacgaat tctaccgttc gtataatgta tgctatacga agttatagat
     4861 ctgtttagct tgcctcgtcc ccgccgggtc acccggccag cgacatggag gcccagaata
     4921 ccctccttga cagtcttgac gtgcgcagct caggggcatg atgtgactgt cgcccgtaca
     4981 tttagcccat acatccccat gtataatcat ttgcatccat acattttgat ggccgcacgg
     5041 cgcgaagcaa aaattacggc tcctcgctgc agacctgcga gcagggaaac gctcccctca
     5101 cagacgcgtt gaattgtccc cacgccgcgc ccctgtagag aaatataaaa ggttaggatt
     5161 tgccactgag gttcttcttt catatacttc cttttaaaat cttgctagga tacagttctc
     5221 acatcacatc cgaacataaa caaccatggg taaggaaaag actcacgttt cgaggccgcg
     5281 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     5341 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     5401 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     5461 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     5521 atggttactc accactgcga tccccggcaa aacagcattc caggtattag aagaatatcc
     5581 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     5641 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     5701 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     5761 tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt
     5821 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     5881 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     5941 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     6001 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcagt
     6061 actgacaata aaaagattct tgttttcaag aacttgtcat ttgtatagtt tttttatatt
     6121 gtagttgttc tattttaatc aaatgttagc gtgatttata ttttttttcg cctcgacatc
     6181 atctgcccag atgcgaagtt aagtgcgcag aaagtaatat catgcgtcaa tcgtatgtga
     6241 atgctggtcg ctatactgct gtcgattcga tactaacgcc gccatccagt gtcgaaaacg
     6301 agctcataac ttcgtataat gtatgctata cgaacggtag aattcgatat cagatccact
     6361 agtggcctat gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6421 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7261 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8581 cccgaaaagt gccacctggg tccttttcat cacgtgctat aaaaataatt ataatttaaa
     8641 ttttttaata taaatatata aattaaaaat agaaagtaaa aaaagaaatt aaagaaaaaa
     8701 tagtttttgt tttccgaaga tgtaaaagac tctaggggga tcgccaacaa atactacctt
     8761 ttatcttgct cttcctgctc tcaggtatta atgccgaatt gtttcatctt gtctgtgtag
     8821 aagaccacac acgaaaatcc tgtgatttta cattttactt atcgttaatc gaatgtatat
     8881 ctatttaatc tgcttttctt gtctaataaa tatatatgta aagtacgctt tttgttgaaa
     8941 ttttttaaac ctttgtttat ttttttttct tcattccgta actcttctac cttctttatt
     9001 tactttctaa aatccaaata caaaacataa aaataaataa acacagagta aattcccaaa
     9061 ttattccatc attaaaagat acgaggcgcg tgtaagttac aggcaagcga tccgtctaag
     9121 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9181 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9241 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9301 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9361 accatagatc acgacattac tatatatata atataggaag catttaatag aacagcatcg
     9421 taatatatgt gtactttgca gttatgacgc cagatggcag tagtggaaga tattctttat
     9481 tgaaaaatag cttgtcacct tacgtacaat cttgatccgg agcttttctt tttttgccga
     9541 ttaagaatta attcggtcga aaaaagaaaa ggagagggcc aagagggagg gcattggtga
     9601 ctattgagca cgtgagtata cgtgattaag cacacaaagg cagcttggag tatgtctgtt
     9661 attaatttca caggtagttc tggtccattg gtgaaagttt gcggcttgca gagcacagag
     9721 gccgcagaat gtgctctaga ttccgatgct gacttgctgg gtattatatg tgtgcccaat
     9781 agaaagagaa caattgaccc ggttattgca aggaaaattt caagtcttgt aaaagcatat
     9841 aaaaatagtt caggcactcc gaaatacttg gttggcgtgt ttcgtaatca acctaaggag
     9901 gatgttttgg ctctggtcaa tgattacggc attgatatcg tccaactgca tggagatgag
     9961 tcgtggcaag aataccaaga gttcctcggt ttgccagtta ttaaaagact cgtatttcca
    10021 aaagactgca acatactact cagtgcagct tcacagaaac ctcattcgtt tattcccttg
    10081 tttgattcag aagcaggtgg gacaggtgaa cttttggatt ggaactcgat ttctgactgg
    10141 gttggaaggc aagagagccc cgaaagctta cattttatgt tagctggtgg actgacgcca
    10201 gaaaatgttg gtgatgcgct tagattaaat ggcgttattg gtgttgatgt aagcggaggt
    10261 gtggagacaa atggtgtaaa agactctaac aaaatagcaa atttcgtcaa aaatgctaag
    10321 aaataggtta ttactgagta gtatttattt aagtattgtt tgtgcacttg ccgatctatg
    10381 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg
    10441 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    10501 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    10561 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    10621 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    10681 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    10741 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    10801 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    10861 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
    10921 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
    10981 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
    11041 tgagcgcgcg
//
