LOCUS       sequence-332212-       10433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10433)
  AUTHORS   Toda S, McKeithan WL, Hakkinen TJ, Lopez P, Klein OD, Lim WA
  TITLE     Engineering synthetic morphogen systems that can program 
            multicellular patterning.
  JOURNAL   Science. 2020 Oct 16;370(6514):327-331. doi: 
            10.1126/science.abc0033.
  PUBMED    33060357
REFERENCE   2  (bases 1 to 10433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2020-10-16- 16"; volume: "370"; 
            issue: "6514"; pages: "327-331"
COMMENT     Sequence Label: pHR_TRE3GS-->sGFP-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..10433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    78..96
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    114..132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    150..168
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    186..204
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    222..240
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(461..463,464..1174)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             1190..1246
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1250..1951
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    2049..2637
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2102..2122)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2520..2531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2640..2656)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2641..2657)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2747..2980
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3005..3023)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3006..3023)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3313..3332)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3546..3565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3665..3687
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3725..3743)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3811..3915
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3916..3984,3985..4776)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4134..4153)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4947..5535
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5436..5455
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5689..5706
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5776..5796)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5781..6110
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5961..6096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7244..7309
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7439..7459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7884..8018
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7921..7940)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7975..7994
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8186..8819
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8866..8991
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9488..9721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9906..9950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10235..10352
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(10431..10433,1..365)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
ORIGIN
        1 tttactccct atcagtgata gagaacgtat gaagagttta ctccctatca gtgatagaga
       61 acgtatgcag actttactcc ctatcagtga tagagaacgt ataaggagtt tactccctat
      121 cagtgataga gaacgtatga ccagtttact ccctatcagt gatagagaac gtatctacag
      181 tttactccct atcagtgata gagaacgtat atccagttta ctccctatca gtgatagaga
      241 acgtataagc tttgcttatg taaaccaggg cgcctataaa agagtgctga ttttttgagt
      301 aaacttcaat tccacaacac ttttgtctta taccaacttt ccgtaccact tcctaccctc
      361 gtaaagaatt cccgtataca ccggtgcaat tctcacgcgt gccgccacca tgggcgttaa
      421 agtccttttc gccctgatct gcattgccgt cgcagaggcc gtttctaagg gtgaagaatt
      481 gttcaccgga gttgtgccca tcctggttga gctggacgga gacgttaacg gacacaagtt
      541 ctcagtgagc ggcgaaggag agggcgacgc tacgtacggt aaactgacgt tgaagttcat
      601 ctgtacaaca ggaaagctgc cagtgccctg gcctacactc gtgaccaccc tcacctacgg
      661 agtccagtgc ttctcccggt accccgacca catgaaacag cacgatttct tcaagtccgc
      721 gatgcctgag ggctatgttc aggaacgcac catattcttc aaggacgacg gtaactataa
      781 aaccagggct gaagttaagt tcgaggggga cacgttggta aatagaattg agttgaaagg
      841 aatcgatttc aaagaagacg gcaatatttt gggccacaag ctggagtaca actacaacag
      901 ccacaacgtg tatattatgg ctgataagca gaagaacggc atcaaggtga acttcaaaat
      961 ccgacacaat atcgaggatg gctctgtgca actggccgac cattaccagc aaaacacgcc
     1021 tatcggcgac ggtccggttt tgctgccaga taaccactac ctctccacgc aatcagccct
     1081 ctccaaggat cctaacgaaa aaagagacca tatggttctg cttgaattcg tgacggctgc
     1141 tgggatcacc ctgggaatgg atgagctgta taaggccagc ggcagcggcg ccacaaactt
     1201 ctctctgcta aagcaagcag gtgatgttga agaaaacccc gggcctcata tgagcgagct
     1261 gattaaggag aacatgcaca tgaagctgta catggagggc accgtggaca accatcactt
     1321 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1381 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacta gcttcctcta
     1441 cggcagcaag accttcatca accacaccca gggcatcccc gacttcttca agcagtcctt
     1501 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1561 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1621 gaacttcaca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccttcac
     1681 cgagacgctg taccccgctg acggcggcct ggaaggcaga aacgacatgg ccctgaagct
     1741 cgtgggcggg agccatctga tcgcaaacat caagaccaca tatagatcca agaaacccgc
     1801 taagaacctc aagatgcctg gcgtctacta tgtggactac agactggaaa gaatcaagga
     1861 ggccaacaac gagacctacg tcgagcagca cgaggtggca gtggccagat actgcgacct
     1921 ccctagcaaa ctggggcaca agcttaatta agcggccgca actcccacct gcaacatgcg
     1981 tgactgactg aggccgcgac tctagagtcg acctgcaggc atgcaagctt gatatcaagc
     2041 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     2101 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     2161 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     2221 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2281 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2341 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2401 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2461 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     2521 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     2581 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     2641 gataccgtcg acctcgaggg aattaattcg agctcggtac ctttaagacc aatgacttac
     2701 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     2761 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     2821 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     2881 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     2941 tccctcagac ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt
     3001 gtattctata gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt
     3061 agccgcgttc taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc
     3121 agaccctatc atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct
     3181 gagtttctgg taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca
     3241 tcgctagcca gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg
     3301 cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg
     3361 ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt
     3421 tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc
     3481 tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact
     3541 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3601 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3661 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3721 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3781 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3841 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3901 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3961 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4021 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4081 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4141 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4201 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4261 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4321 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4381 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4441 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4501 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4561 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4621 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4681 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4741 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4801 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4861 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4921 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4981 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5041 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5101 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5161 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5221 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5281 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5341 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5401 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5461 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5521 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5581 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     5641 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     5701 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     5761 cattaatgca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5821 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5881 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5941 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6001 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     6061 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac
     6121 acaagacagg cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc
     6181 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat
     6241 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     6301 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     6361 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     6421 ggcggtctgt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
     6481 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
     6541 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg
     6601 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
     6661 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc
     6721 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
     6781 tggcactgcc gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt
     6841 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
     6901 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
     6961 gtgcagtcgg cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
     7021 atgtggatct ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg
     7081 atgagcgatg ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc
     7141 ggcaactgga tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt
     7201 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     7261 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     7321 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     7381 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     7441 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     7501 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     7561 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     7621 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     7681 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     7741 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     7801 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     7861 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7921 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7981 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     8041 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     8101 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     8161 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     8221 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8281 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8341 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8401 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8461 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8521 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8581 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     8641 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     8701 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     8761 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8821 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     8881 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     8941 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     9001 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     9061 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     9121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     9181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     9241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     9301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     9361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     9421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     9481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     9541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     9601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     9661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     9721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     9781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     9841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     9901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     9961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
    10021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
    10081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
    10141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
    10201 gacggtatcg ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
    10261 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
    10321 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
    10381 cagtttggat cgataagctt gatatcgaat tcccgtggaa ttatcacctc gag
//
