LOCUS       sequence-33238-e         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCI Flag Smad3 NL* EPSM
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(637..660)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(678..696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(739..758)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          741..873
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 ggccgcacct gcgtccatgc tgtggttcat ctggtggtca ctggtttctc catcttcact
       61 caggtagcca gggggaggga cctctggaat attgctctgg ggctcgatgc ctgcggggaa
      121 gttagtgttt tcggggatgg aatggctgta gtcgtccagt ggggggaact cggccgggat
      181 ctctgtgtgg cgtggcacca acacaggagg tagaactggt gtctctactc tctggtagtg
      241 gtagggattc acgcagacct cgtccttctt catattgaag gcgaactcac acagctccat
      301 ggcccgcagc tcgtggtggc tgtgcaggtc tggccatcgc cacaggcggc agtagatgac
      361 atgagggagc cccttccgat gggacacctg caaccggcca tccagggacc tggggatggt
      421 gatgcacttg gtgttgacgt tctgcgtggt gatggccttc tccagctcgt ccagctgccc
      481 cgtcttcttg agtttcttga ccaggctctt gaccgccttc tcgcaccatt tctcctcctg
      541 cccgttctgc tcgcccttct tccagcccag caggcgcttc acgatcgggg gagtgaaagg
      601 caggatggac gatctagagg taccacgcgt gaattctttg tcatcgtcat ccttatagtc
      661 catggtctcg aggctagcct atagtgagtc gtattaagta ctctagcctt aagagctgta
      721 attgaactgg gagtggacac ctgtggagag aaaggcaaag tggatgtcag taagaccaat
      781 aggtgcctat cagaaacgca agantcttct ctgtctcgac aagcccagtt tctattggtc
      841 tccttaaacc tgtcttgtaa ccttgatact tacntgccca gtgcctcacg accaacttct
      901 gcagctaaat ttcgagantg ttgtgtcata aacactgact gctaca
//
