LOCUS       sequence-332467-        9968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express hPOT1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9968)
  AUTHORS   Pinzaru AM, Kareh M, Lamm N, Lazzerini-Denchi E, Cesare AJ, Sfeir A
  TITLE     Replication stress conferred by POT1 dysfunction promotes telomere 
            relocalization to the nuclear pore.
  JOURNAL   Genes Dev. 2020 Dec 1;34(23-24):1619-1636. doi: 
            10.1101/gad.337287.120. Epub 2020 Oct 29.
  PUBMED    33122293
REFERENCE   2  (bases 1 to 9968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2020-12-1"; volume: "34(23-24):1619-1636. doi"; pages:
            " 10.1101/gad.337287.120. Epub 2020 Oct 29"
COMMENT     Sequence Label: pHAGE2-EF1a-MCS-hPOT1_IRES-blast
FEATURES             Location/Qualifiers
     source          1..9968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3443
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    5487..5948
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5543..5560)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5770..5789
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    6410..6998
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6463..6483)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6881..6892)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7146..7326
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7373..7391)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7459..7563
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7564..7632,7633..8424)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7782..7801)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8595..9183
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9337..9354
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9594..9615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9630..9635,9636..9653,9654..9660)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9668..9684
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9673..9695
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9692..9708
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9692..9708
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9758..9777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagcttcagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atggaacaaa
     3421 agttgatttc tgaagaagat ttgttaagaa agagaggatc cttggttcca gcaacaaatt
     3481 atatatatac acccctgaat caacttaagg gtggtacaat tgtcaatgtc tatggtgttg
     3541 tgaagttctt taagccccca tatctaagca aaggaactga ttattgctca gttgtaacta
     3601 ttgtggacca gacaaatgta aaactaactt gcctgctctt tagtggaaac tatgaagccc
     3661 ttccaataat ttataaaaat ggagatattg ttcgctttca caggctgaag attcaagtat
     3721 ataaaaagga gactcagggt atcaccagct ctggctttgc atctttgacg tttgagggaa
     3781 ctttgggagc ccctatcata cctcgcactt caagcaagta ttttaacttc actactgagg
     3841 accacaaaat ggtagaagcc ttacgtgttt gggcatctac tcatatgtca ccgtcttgga
     3901 cattactaaa attgtgtgat gttcagccaa tgcagtattt tgacctgact tgtcagctct
     3961 tgggcaaagc agaagtggac ggagcatcat ttcttctaaa ggtatgggat ggcaccagga
     4021 caccatttcc atcttggaga gtcttaatac aagaccttgt tcttgaaggt gatttaagtc
     4081 acatccatcg gctacaaaat ctgacaatag acattttagt ctacgataac catgttcatg
     4141 tggcaagatc tctgaaggtt ggaagctttc ttagaatcta tagccttcat accaaacttc
     4201 aatcaatgaa ttcagagaat cagacaatgt taagtttaga gtttcatctt catggaggta
     4261 ccagttacgg tcggggaatc agggtcttgc cagaaagtaa ctctgatgtg gatcaactga
     4321 aaaaggattt agaatctgca aatttgacag ccaatcagca ttcagatgtt atctgtcaat
     4381 cagaacctga cgacagcttt ccaagctctg gatcagtatc attatacgag gtagaaagat
     4441 gtcaacagct atctgctaca atacttacag atcatcagta tttggagagg acaccactat
     4501 gtgccatttt gaaacaaaaa gctcctcaac aataccgcat ccgagcaaaa ttgaggtcat
     4561 ataagcccag aagactattt cagtctgtta aacttcattg ccctaaatgt catttgctgc
     4621 aagaagttcc acatgagggc gatttggata taatttttca ggatggtgca actaaaaccc
     4681 cagatgtcaa gctacaaaat acatcattat atgattcaaa aatctggacc actaaaaatc
     4741 aaaaaggacg aaaagtagca gttcattttg tgaaaaataa tggtattctc ccgctttcaa
     4801 atgaatgtct acttttgata gaaggaggta cactcagtga aatttgcaaa ctctcgaaca
     4861 agtttaatag tgtaattcct gtgagatctg gccacgaaga cctggaactt ttggaccttt
     4921 cagcaccatt tcttatacaa ggaacaatac atcactatgg atgtaaacag tgttctagtt
     4981 tgagatccat acaaaatcta aattccctgg ttgataaaac atcgtggatt ccttcttctg
     5041 tggcagaagc actgggtatt gtacccctcc aatatgtgtt tgttatgacc tttacacttg
     5101 atgatggaac aggagtacta gaagcctatc tcatggattc tgacaaattc ttccagattc
     5161 cagcatcaga agttctgatg gatgatgacc ttcagaaaag tgtggatatg atcatggata
     5221 tgttttgtcc tccaggaata aaaattgatg catatccgtg gttggaatgc ttcatcaagt
     5281 catacaatgt cacaaatgga acagataatc aaatttgcta tcagattttt gacaccacag
     5341 ttgcagaaga tgtaatctaa ttaattaagt ttaaacggat cccgccggcg gctagcgtta
     5401 acacgcgttg tacattttac gtatctagag ctcgaggtcg accaattccg gttattttcc
     5461 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     5521 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     5581 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     5641 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     5701 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     5761 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     5821 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     5881 aggttaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5941 gatgataata ccatgaaaac atttaacatt tctcaacagg atctagaatt agtagaagta
     6001 gcgacagaga agattacaat gctttatgag gataataaac atcatgtggg agcggcaatt
     6061 cgtacgaaaa caggagaaat catttcggca gtacatattg aagcgtatat aggacgagta
     6121 actgtttgtg cagaagccat tgcgattggt agtgcagttt cgaatggaca aaaggatttt
     6181 gacacgattg tagctgttag acacccttat tctgacgaag tagatagaag tattcgagtg
     6241 gtaagtcctt gtggtatgtg tagggagttg atttcagact atgcaccaga ttgttttgtg
     6301 ttaatagaaa tgaatggcaa gttagtcaaa actacgattg aagaactcat tccactcaaa
     6361 tatacccgaa attaaaagtt ttaccatacc aagctcatcg atagatccta atcaacctct
     6421 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6481 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6541 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6601 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6661 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6721 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6781 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6841 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     6901 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     6961 tcagacgagt cggatctccc tttgggccgc ctccccgcct ggtaccttta agaccaatga
     7021 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7081 taattcactc ccaacgaaga caagatcacc tgcaggacag gcgcgccctg ctttttgctt
     7141 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7201 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7261 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7321 ctagcacccg ggcgattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     7381 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7441 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7501 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7561 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7621 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7681 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7741 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     7801 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7861 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7921 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7981 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8041 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8101 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8161 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8221 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8281 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8341 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8401 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8461 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8521 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8581 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8641 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8701 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     8761 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8821 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8881 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8941 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9001 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9061 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9121 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9181 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     9241 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     9301 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     9361 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     9421 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     9481 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9541 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     9601 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     9661 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
     9721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9781 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9841 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9901 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9961 ttagtagt
//
